Characterization of extracellular polymeric substances produced by mixed microorganisms in activated sludge with gel-permeating chromatography, excitation–emission matrix fluorescence spectroscopy measurement and kinetic modeling

2009 ◽  
Vol 43 (5) ◽  
pp. 1350-1358 ◽  
Author(s):  
Bing-Jie Ni ◽  
Fang Fang ◽  
Wen-Ming Xie ◽  
Min Sun ◽  
Guo-Ping Sheng ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Activated Sludge ◽  
Kinetic Modeling ◽  
Extracellular Polymeric Substances ◽  
Excitation Emission Matrix ◽  
Spectroscopy Measurement

Characterization of interactions between a metabolic uncoupler O-chlorophenol and extracellular polymeric substances of activated sludge

2019 ◽  
Vol 247 ◽  
pp. 1020-1027 ◽  
Author(s):  
Fang Fang ◽  
Run-Ze Xu ◽  
Su-Na Wang ◽  
Lu-Lu Zhang ◽  
Yan-Qiu Huang ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Extracellular Polymeric Substances

Interaction between Enrofloxacin and the Extracellular Polymeric Substances from Platymonas Subcordiformis

2014 ◽  
Vol 1015 ◽  
pp. 531-535
Author(s):  
Li Yun Ge ◽  
Shu Juan Li ◽  
Chang Rong Zhao ◽  
Huan Huan Deng
Keyword(s):  
Fluorescence Spectroscopy ◽  
Fluorescence Intensity ◽  
Aquatic Environment ◽  
Marine Algae ◽  
Extracellular Polymeric Substances ◽  
Platymonas Subcordiformis ◽  
Excitation Emission Matrix ◽  
Quinolone Antibiotics

Extracellular polymeric substances (EPS) in marine algae-Platymonas subcordiformisaffected by enrofloxacin (ENR), the typical quinolone antibiotics released to the environment through different ways, was studied. A 3-dimension EEM (excitation –emission matrix) fluorescence spectroscopy was used to examine the interaction between EPS and ENR, and the fluorescent peaks A and B were detected, whose fluorescence intensity remarkably decreased with the increased ENR concentration. It was demonstrated that the interaction of EPS with ENR well fitted the modified Stern-Volmer. It was concluded that the presence of EPS in marine algae affected the transport and transform of antibiotics in aquatic environment.

Three-dimensional excitation emission matrix fluorescence spectroscopy and gel-permeating chromatography to characterize extracellular polymeric substances in aerobic granulation

2010 ◽  
Vol 61 (11) ◽  
pp. 2931-2942 ◽  
Author(s):  
Huacheng Xu ◽  
Pinjing He ◽  
Guanzhao Wang ◽  
Liming Shao
Keyword(s):  
Molecular Weight ◽  
Fluorescence Spectroscopy ◽  
Extracellular Polymeric Substances ◽  
Operating Time ◽  
Three Dimensional ◽  
Aerobic Granules ◽  
Aerobic Granulation ◽  
Excitation Emission Matrix ◽  
Two Peaks ◽  
Eem Fluorescence

Three-dimensional excitation emission matrix (EEM) fluorescence spectroscopy and gel-permeating chromatography (GPC) were employed to characterize the extracellular polymeric substances (EPS) in aerobic granulation. EPS matrix in this study was stratified into four fractions: (1) supernatant, (2) slime, (3) loosely bound EPS (LB-EPS), and (4) tightly bound EPS (TB-EPS). The results showed that the dissolved organic carbon was mainly distributed in TB-EPS fraction, and increased with increasing the operating time. The supernatant, slime, and LB-EPS fractions exhibited four fluorescence peaks, an autochthonous signature, unimodal MW distribution and lower molecular weight (MW) (3 < log [MW]<5), whereas the TB-EPS fraction only had two peaks, an allochthonous signature, multiple peaks and higher MW (5 < log [MW]<7). It was deemed that the formation of aerobic granules was correlated with the accumulation of proteins in the TB-EPS fraction. EEM spectroscopy and GPC profiles could be used as appropriate and effective methods to characterize the EPS in aerobic granulation from a micro-view level.

Brown carbon aerosols in the Indo-Gangetic Plain outflow: insights from excitation emission matrix (EEM) fluorescence spectroscopy

2021 ◽  
Author(s):  
Supriya Dey ◽  
Arya Mukherjee ◽  
Anuraag J Polana ◽  
Archita Rana ◽  
Jingying Mao ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Gangetic Plain ◽  
Brown Carbon ◽  
Excitation Emission Matrix ◽  
Indian Context ◽  
Parallel Factor ◽  
Carbon Aerosols ◽  
Indo Gangetic Plain ◽  
Eem Fluorescence

We report the first characterization of aerosol brown carbon (BrC) composition in the Indian context using excitation emission matrix (EEM) fluorescence spectroscopy coupled with parallel factor (PARAFAC) analysis. We find...

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