scholarly journals A comparison of five extraction methods for extracellular polymeric substances (EPS) from biofilm by using threedimensional excitation-emission matrix (3DEEM) fluorescence spectroscopy

Water SA ◽  
2010 ◽  
Vol 36 (1) ◽  
Author(s):  
X Pan ◽  
J Liu ◽  
D Zhang ◽  
X Chen ◽  
L Li ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Extracellular Polymeric Substances ◽  
Extraction Methods ◽  
Excitation Emission Matrix

Interaction between Enrofloxacin and the Extracellular Polymeric Substances from Platymonas Subcordiformis

2014 ◽  
Vol 1015 ◽  
pp. 531-535
Author(s):  
Li Yun Ge ◽  
Shu Juan Li ◽  
Chang Rong Zhao ◽  
Huan Huan Deng
Keyword(s):  
Fluorescence Spectroscopy ◽  
Fluorescence Intensity ◽  
Aquatic Environment ◽  
Marine Algae ◽  
Extracellular Polymeric Substances ◽  
Platymonas Subcordiformis ◽  
Excitation Emission Matrix ◽  
Quinolone Antibiotics

Extracellular polymeric substances (EPS) in marine algae-Platymonas subcordiformisaffected by enrofloxacin (ENR), the typical quinolone antibiotics released to the environment through different ways, was studied. A 3-dimension EEM (excitation –emission matrix) fluorescence spectroscopy was used to examine the interaction between EPS and ENR, and the fluorescent peaks A and B were detected, whose fluorescence intensity remarkably decreased with the increased ENR concentration. It was demonstrated that the interaction of EPS with ENR well fitted the modified Stern-Volmer. It was concluded that the presence of EPS in marine algae affected the transport and transform of antibiotics in aquatic environment.

Three-dimensional excitation emission matrix fluorescence spectroscopy and gel-permeating chromatography to characterize extracellular polymeric substances in aerobic granulation

2010 ◽  
Vol 61 (11) ◽  
pp. 2931-2942 ◽  
Author(s):  
Huacheng Xu ◽  
Pinjing He ◽  
Guanzhao Wang ◽  
Liming Shao
Keyword(s):  
Molecular Weight ◽  
Fluorescence Spectroscopy ◽  
Extracellular Polymeric Substances ◽  
Operating Time ◽  
Three Dimensional ◽  
Aerobic Granules ◽  
Aerobic Granulation ◽  
Excitation Emission Matrix ◽  
Two Peaks ◽  
Eem Fluorescence

Three-dimensional excitation emission matrix (EEM) fluorescence spectroscopy and gel-permeating chromatography (GPC) were employed to characterize the extracellular polymeric substances (EPS) in aerobic granulation. EPS matrix in this study was stratified into four fractions: (1) supernatant, (2) slime, (3) loosely bound EPS (LB-EPS), and (4) tightly bound EPS (TB-EPS). The results showed that the dissolved organic carbon was mainly distributed in TB-EPS fraction, and increased with increasing the operating time. The supernatant, slime, and LB-EPS fractions exhibited four fluorescence peaks, an autochthonous signature, unimodal MW distribution and lower molecular weight (MW) (3 < log [MW]<5), whereas the TB-EPS fraction only had two peaks, an allochthonous signature, multiple peaks and higher MW (5 < log [MW]<7). It was deemed that the formation of aerobic granules was correlated with the accumulation of proteins in the TB-EPS fraction. EEM spectroscopy and GPC profiles could be used as appropriate and effective methods to characterize the EPS in aerobic granulation from a micro-view level.

Optimization for extracellular polymeric substances extraction of microbial aggregates

2015 ◽  
Vol 71 (7) ◽  
pp. 1106-1112 ◽  
Author(s):  
Liang Zhu ◽  
Haitian Yu ◽  
Yimei Liu ◽  
Hanying Qi ◽  
Xiangyang Xu
Keyword(s):  
Humic Acid ◽  
Extracellular Polymeric Substances ◽  
Three Dimensional ◽  
Interaction Mechanism ◽  
Extraction Methods ◽  
Aerobic Granules ◽  
Excitation Emission Matrix ◽  
Cation Exchange Resins ◽  
Heat Method ◽  
Exchange Resins

The extracellular polymeric substances (EPS) are important macromolecular components in microbial aggregates. The three EPS extraction methods – ultrasound + cation exchange resins (CER) + sulfide, ultrasound + formamide + NaOH, and ultrasound + heat – were investigated in the study, and the component differences of extracted EPS from the loose flocs and dense aerobic granules were compared using chemical analysis and three-dimensional excitation-emission matrix (3D-EEM). Results showed that the contents of EPS were extracted effectively by ultrasound + formamide + NaOH and ultrasound + heat methods, and the ultrasound + CER + sulfide method did not extract the polysaccharides (PS) or protein (PN) contents from the sludge samples. The 3D-EEM analysis indicated that the nature of peak B/D, peak C/E/F, and peak A/G were attributed to PN-like, humic acid-like and fulvic acid-like fluorophores. All fluorophores can be detected from the EPS extracted through the ultrasound + heat method. Hopefully this will provide more information about the EPS interaction mechanism of microbial aggregates.

Quantitative assessment of extraction methods for bound extracellular polymeric substances (B-EPSs) produced by Microcystis sp. and Scenedesmus sp.

2021 ◽  
Vol 56 ◽  
pp. 102289
Author(s):  
Ruixing Huang ◽  
Qiang He ◽  
Jun Ma ◽  
Chengxue Ma ◽  
Yanghui Xu ◽  
...  
Keyword(s):  
Quantitative Assessment ◽  
Extracellular Polymeric Substances ◽  
Extraction Methods

scholarly journals Identification of resistance in Escherichia coli and Klebsiella pneumoniae using excitation-emission matrix fluorescence spectroscopy and multivariate analysis

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Fernanda S. L. Costa ◽  
Caio C. R. Bezerra ◽  
Renato M. Neto ◽  
Camilo L. M. Morais ◽  
Kássio M. G. Lima
Keyword(s):  
Escherichia Coli ◽  
Multivariate Analysis ◽  
Klebsiella Pneumoniae ◽  
Fluorescence Spectroscopy ◽  
Excitation Emission Matrix

Diagnosis of the unexpected fluorescent contaminants in quantifying dissolved organic matter using excitation-emission matrix fluorescence spectroscopy

2019 ◽  
Vol 163 ◽  
pp. 114873 ◽  
Author(s):  
Chen Qian ◽  
Wei Chen ◽  
Bo Gong ◽  
Long-Fei Wang ◽  
Han-Qing Yu
Keyword(s):  
Organic Matter ◽  
Fluorescence Spectroscopy ◽  
Dissolved Organic Matter ◽  
Excitation Emission Matrix

Evaluation of natural organic matter changes from Lake Hohloh by three-dimensional excitation–emission matrix fluorescence spectroscopy during TiO2/UV process

2014 ◽  
Vol 51 ◽  
pp. 124-133 ◽  
Author(s):  
Sergio Valencia ◽  
Juan M. Marín ◽  
Gloria Restrepo ◽  
Fritz H. Frimmel
Keyword(s):  
Organic Matter ◽  
Fluorescence Spectroscopy ◽  
Natural Organic Matter ◽  
Three Dimensional ◽  
Excitation Emission Matrix

Excitation-Emission-Matrix Fluorescence Spectroscopy of Soil Water Extracts to Predict Nitrogen Mineralization Rates

2018 ◽  
Vol 82 (1) ◽  
pp. 126-135 ◽  
Author(s):  
Oshri Rinot ◽  
William R. Osterholz ◽  
Michael J. Castellano ◽  
Raphael Linker ◽  
Matt Liebman ◽  
...  
Keyword(s):  
Fluorescence Spectroscopy ◽  
Soil Water ◽  
Nitrogen Mineralization ◽  
Water Extracts ◽  
Excitation Emission Matrix
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