Interaction between Enrofloxacin and the Extracellular Polymeric Substances from Platymonas Subcordiformis

2014 ◽  
Vol 1015 ◽  
pp. 531-535
Author(s):  
Li Yun Ge ◽  
Shu Juan Li ◽  
Chang Rong Zhao ◽  
Huan Huan Deng

Extracellular polymeric substances (EPS) in marine algae-Platymonas subcordiformisaffected by enrofloxacin (ENR), the typical quinolone antibiotics released to the environment through different ways, was studied. A 3-dimension EEM (excitation –emission matrix) fluorescence spectroscopy was used to examine the interaction between EPS and ENR, and the fluorescent peaks A and B were detected, whose fluorescence intensity remarkably decreased with the increased ENR concentration. It was demonstrated that the interaction of EPS with ENR well fitted the modified Stern-Volmer. It was concluded that the presence of EPS in marine algae affected the transport and transform of antibiotics in aquatic environment.

2010 ◽  
Vol 61 (11) ◽  
pp. 2931-2942 ◽  
Author(s):  
Huacheng Xu ◽  
Pinjing He ◽  
Guanzhao Wang ◽  
Liming Shao

Three-dimensional excitation emission matrix (EEM) fluorescence spectroscopy and gel-permeating chromatography (GPC) were employed to characterize the extracellular polymeric substances (EPS) in aerobic granulation. EPS matrix in this study was stratified into four fractions: (1) supernatant, (2) slime, (3) loosely bound EPS (LB-EPS), and (4) tightly bound EPS (TB-EPS). The results showed that the dissolved organic carbon was mainly distributed in TB-EPS fraction, and increased with increasing the operating time. The supernatant, slime, and LB-EPS fractions exhibited four fluorescence peaks, an autochthonous signature, unimodal MW distribution and lower molecular weight (MW) (3 < log [MW]<5), whereas the TB-EPS fraction only had two peaks, an allochthonous signature, multiple peaks and higher MW (5 < log [MW]<7). It was deemed that the formation of aerobic granules was correlated with the accumulation of proteins in the TB-EPS fraction. EEM spectroscopy and GPC profiles could be used as appropriate and effective methods to characterize the EPS in aerobic granulation from a micro-view level.


2018 ◽  
Vol 82 (1) ◽  
pp. 126-135 ◽  
Author(s):  
Oshri Rinot ◽  
William R. Osterholz ◽  
Michael J. Castellano ◽  
Raphael Linker ◽  
Matt Liebman ◽  
...  

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