Cryptic lineage diversity in the zoonotic pathogen Angiostrongylus cantonensis

2017 ◽  
Vol 107 ◽  
pp. 404-414 ◽  
Author(s):  
Sirilak Dusitsittipon ◽  
Charles D. Criscione ◽  
Serge Morand ◽  
Chalit Komalamisra ◽  
Urusa Thaenkham
2014 ◽  
Vol 43 (5) ◽  
pp. 472-484 ◽  
Author(s):  
Kanishka D. B. Ukuwela ◽  
Anslem de Silva ◽  
Mumpuni ◽  
Bryan G. Fry ◽  
Kate L. Sanders

Evolution ◽  
2017 ◽  
Vol 72 (1) ◽  
pp. 54-66 ◽  
Author(s):  
Craig C. Moritz ◽  
Renae C. Pratt ◽  
Sarah Bank ◽  
Gayleen Bourke ◽  
Jason G. Bragg ◽  
...  

Author(s):  
Qiao-Ping Wang ◽  
Zhao-Rong Lun

Angiostrongylus cantonensis was first discovered in rats in Guangzhou (Canton), China in 1935 (Chen 1935). A. cantonensis is a zoonotic pathogen, which causes human angiostrongylosis with the main clinical manifestation of eosinophilic meningitis. The first case of human angiostrongylosis was reported in Taiwan in 1945. Subsequently several outbreaks of this disease occurred in Pacific Islands (Rosen et al. 1961; Kliks and Palumbo 1992). In the past decade, a number of outbreaks of human angiostrongylosis have emerged in some endemic regions, especially in China (Wang et al. 2008). Additionally, increasing numbers of travellers are diagnosed with eosinophilic meningitis caused by A. cantonensis after returning from endemic regions (Lo et al. 2001; Slom et al. 2002; Bartschi et al. 2004; Podwall et al. 2004; Kumar et al. 2005; Leone et al. 2007; Ali et al. 2008). The parasite continues to threaten human beings, especially people living in the Pacific Islands and Asia. So far, at least 2,825 cases have been recorded; of them, 1,337 were reported in Thailand, 769 in China (Hong Kong and Taiwan), 256 in Tahiti, 116 in the USA (Hawaii and Samoa) and 114 cases in Cuba (Wang et al. 2008).


2009 ◽  
Vol 48 (12) ◽  
pp. 632
Author(s):  
Bárbara Padilla Docal ◽  
Alberto Juan Dorta Contreras ◽  
Raisa Bu-Coifiú Fanego

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Cheng Yang ◽  
Wangfeng Shao ◽  
Lingling Wei ◽  
Lingxiao Chen ◽  
Aihua Zhu ◽  
...  

AbstractSalmonella, as a zoonotic pathogen, has attracted widespread attention worldwide, especially in the transmission between household pets and humans. Therefore, we investigated the epidemic distribution of dog Salmonella from pet hospitals and breeding base in Xuzhou, Jiangsu Province, China, and used multilocus sequence typing (MLST) and clustered regularly interspaced short palindromic repeats (CRISPRs) to subtype Salmonella isolates. From April 2018 to November 2019, a total of 469 samples were collected from pet hospitals and breeding base, including 339 dog samples and 60 cat samples. S. Kentucky (40.74%) was the most prevalent serotype, but other, such as S. Typhimurium (18.52%) and S. Indiana (18.52%), were also widespread. Eight different sequence type (ST) patterns were identified by MLST and ST198 was the highest proportion of these isolates. CRISPRs analysis showed that 9 different Kentucky CRISPR types (KCTs) was identified from ST198. 48 spacers including 29 (6 News) for CRISPR1 and 19 (4 News) for CRISPR2 that proved the polymorphic of Salmonella genes in samples from different sources. The analysis demonstrated that the common serotypes were widely present in pet hosts in the same area. This analysis shows that CRISPR genes have better recognition ability in the same serotype, which has a positive effect on the traceability of Salmonella and the prevention and treatment of salmonellosis.


Author(s):  
Hongli Zhou ◽  
Minyu Zhou ◽  
Yue Hu ◽  
Yanin Limpanon ◽  
Yubin Ma ◽  
...  

AbstractAngiostrongylus cantonensis (AC) can cause severe eosinophilic meningitis or encephalitis in non-permissive hosts accompanied by apoptosis and necroptosis of brain cells. However, the explicit underlying molecular basis of apoptosis and necroptosis upon AC infection has not yet been elucidated. To determine the specific pathways of apoptosis and necroptosis upon AC infection, gene set enrichment analysis (GSEA) and protein–protein interaction (PPI) analysis for gene expression microarray (accession number: GSE159486) of mouse brain infected by AC revealed that TNF-α likely played a central role in the apoptosis and necroptosis in the context of AC infection, which was further confirmed via an in vivo rescue assay after treating with TNF-α inhibitor. The signalling axes involved in apoptosis and necroptosis were investigated via immunoprecipitation and immunoblotting. Immunofluorescence was used to identify the specific cells that underwent apoptosis or necroptosis. The results showed that TNF-α induced apoptosis of astrocytes through the RIP1/FADD/Caspase-8 axis and induced necroptosis of neurons by the RIP3/MLKL signalling pathway. In addition, in vitro assay revealed that TNF-α secretion by microglia increased upon LSA stimulation and caused necroptosis of neurons. The present study provided the first evidence that TNF-α was secreted by microglia stimulated by AC infection, which caused cell death via parallel pathways of astrocyte apoptosis (mediated by the RIP1/FADD/caspase-8 axis) and neuron necroptosis (driven by the RIP3/MLKL complex). Our research comprehensively elucidated the mechanism of cell death after AC infection and provided new insight into targeting TNF-α signalling as a therapeutic strategy for CNS injury.


Science ◽  
2021 ◽  
pp. eabf2946
Author(s):  
Louis du Plessis ◽  
John T. McCrone ◽  
Alexander E. Zarebski ◽  
Verity Hill ◽  
Christopher Ruis ◽  
...  

The UK’s COVID-19 epidemic during early 2020 was one of world’s largest and unusually well represented by virus genomic sampling. Here we reveal the fine-scale genetic lineage structure of this epidemic through analysis of 50,887 SARS-CoV-2 genomes, including 26,181 from the UK sampled throughout the country’s first wave of infection. Using large-scale phylogenetic analyses, combined with epidemiological and travel data, we quantify the size, spatio-temporal origins and persistence of genetically-distinct UK transmission lineages. Rapid fluctuations in virus importation rates resulted in >1000 lineages; those introduced prior to national lockdown tended to be larger and more dispersed. Lineage importation and regional lineage diversity declined after lockdown, while lineage elimination was size-dependent. We discuss the implications of our genetic perspective on transmission dynamics for COVID-19 epidemiology and control.


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