553. Silencing HBV Gene Expression with Pol II and Pol III Promoter-Derived Short Hairpin RNA

ABSTRACT We demonstrate a novel approach for coexpression of a short hairpin RNA (shRNA) with an open reading frame which exploits transcriptional read-through of a minimal polyadenylation signal from a Pol II promoter. We first observed efficient inducible expression of enhanced green fluorescent protein along with an anti-rev shRNA. We took advantage of this observation to test coexpression of the transdominant negative mutant (humanized) of human immunodeficiency type 1 (HIV-1) Rev (huRevM10) along with an anti-rev shRNA via an HIV-1-inducible fusion promoter. The coexpression of the shRNA and transdominant protein resulted in potent, long-term inhibition of HIV-1 gene expression and suppression of shRNA-resistant mutants. This dual expression system has broad-based potential for other shRNA applications, such as cases where simultaneous knockdown of mutant and wild-type transcripts must be accompanied by replacement of the wild-type protein.

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AB115. Effects of target gene expression on ex vivo differentiated mesenchymal stromal cells transfected by lentiviral vector with PDE5 of short hairpin RNA

Translational Andrology and Urology ◽

10.21037/tau.2016.s115 ◽

2016 ◽

Vol 5 (S1) ◽

pp. AB115-AB115

Author(s):

Heng-Jun Xiao ◽

Weixin Yan ◽

Jun Chen ◽

Xin Gao ◽

Li Zhuan ◽

...

Keyword(s):

Gene Expression ◽

Mesenchymal Stromal Cells ◽

Stromal Cells ◽

Target Gene ◽

Lentiviral Vector ◽

Ex Vivo ◽

Short Hairpin Rna ◽

Hairpin Rna ◽

Target Gene Expression ◽

Short Hairpin

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339. Short Hairpin RNA Delivered by Adeno-Associated Virus Vector Efficiently Reduced Target Gene Expression in Mouse Primary Cortical Neurons

Molecular Therapy ◽

10.1016/s1525-0016(16)34674-3 ◽

2013 ◽

Vol 21 ◽

pp. S131

Keyword(s):

Gene Expression ◽

Cortical Neurons ◽

Target Gene ◽

Short Hairpin Rna ◽

Virus Vector ◽

Hairpin Rna ◽

Primary Cortical Neurons ◽

Adeno Associated Virus ◽

Target Gene Expression ◽

Short Hairpin

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Short hairpin RNA-mediated inhibition of HSV-1 gene expression and function during HSV-1 infection in Vero cells

Acta Pharmacologica Sinica ◽

10.1111/j.1745-7254.2008.00828.x ◽

2008 ◽

Vol 29 (8) ◽

pp. 975-982 ◽

Cited By ~ 3

Author(s):

Yuan-yuan LIU ◽

Hai-ying DENG ◽

Guang YANG ◽

Wen-lin JIANG ◽

Laurent GROSSIN ◽

...

Keyword(s):

Gene Expression ◽

Vero Cells ◽

Short Hairpin Rna ◽

Hairpin Rna ◽

Short Hairpin ◽

And Function ◽

Hsv 1

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Delivery of Short Hairpin RNA Sequences by Using a Replication-Competent Avian Retroviral Vector

Journal of Virology ◽

10.1128/jvi.78.9.4914-4916.2004 ◽

2004 ◽

Vol 78 (9) ◽

pp. 4914-4916 ◽

Cited By ~ 24

Author(s):

Jennifer L. Bromberg-White ◽

Craig P. Webb ◽

Veronique S. Patacsil ◽

Cindy K. Miranti ◽

Bart O. Williams ◽

...

Keyword(s):

Gene Expression ◽

Retroviral Vectors ◽

Retroviral Vector ◽

Short Hairpin Rna ◽

Hairpin Rna ◽

Rna Sequences ◽

Inhibit Gene Expression ◽

Short Hairpin ◽

The Creation

ABSTRACT While recent studies have demonstrated that retroviral vectors can be used to stably express short hairpin RNA (shRNA) to inhibit gene expression, these studies have utilized replication-defective retroviruses. We describe the creation of a replication-competent, Gateway-compatible retroviral vector capable of expressing shRNA that inhibits the expression of specific genes.

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