Interaction of enkephalins with oxyradicals11Abbreviations: ABAP, 2,2′-azobis(2-amidinopropane); dopa, dihydroxyphenyl-alanine; H2O2, hydrogen peroxide; leu-enk, leu-enkephalin; met-enk, met-enkephalin; LOOH, linoleic acid 13-hydroperoxide; NBT, nitro blue tetrazolium; PMS, phenazine methosulfate; ROS, reactive oxygen species; SOD, superoxide dismutase; TBARS, thiobarbituric acid reactive substances

2001 ◽  
Vol 61 (10) ◽  
pp. 1253-1257 ◽  
Author(s):  
Mario Fontana ◽  
Luciana Mosca ◽  
Maria Anna Rosei
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Linoleic Acid ◽  
Reactive Oxygen ◽  
Thiobarbituric Acid ◽  
Oxygen Species ◽  
Phenazine Methosulfate ◽  
Thiobarbituric Acid Reactive Substances ◽  
Ros Reactive Oxygen Species

Intracellular generation of reactive oxygen species during nonhypoxic lung ischemia

1997 ◽  
Vol 272 (2) ◽  
pp. L294-L300 ◽  
Author(s):  
A. B. Al-Mehdi ◽  
H. Shuman ◽  
A. B. Fisher
Keyword(s):  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Reactive Oxygen ◽  
Thiobarbituric Acid ◽  
Conjugated Dienes ◽  
Thiobarbituric Acid Reactive Substance ◽  
Oxygen Species ◽  
Reactive Substance ◽  
Rat Lungs

Surface fluorometry with 40 microM hydroethidine (HE) as a probe was used to detect oxidant generation in isolated, ventilated rat lungs during lung ischemia. Ethidium fluorescence due to HE oxidation was continuously monitored with 470 nm excitation and 610 nm emission. Fluorescence increased with ischemia in O2-ventilated lungs [0.98 +/- 0.08 arbitrary fluorescence units (AFU)/min vs. 0.58 +/- 0.07 with control perfusion]. HE oxidation during ischemia was prevented by N2 ventilation but was unaltered by preperfusion with superoxide dismutase. Ethidium fluorescence in homogenate prepared from lungs subjected to 1 h of nonhypoxic ischemia was increased (16.8 +/- 1.5 vs. 9.8 +/- 0.4 AFU/mg protein in control) but was unchanged in lungs that had been N2 ventilated. Microfluorographs of HE perfused and fixed lung sections demonstrated marked generalized increases in ethidium fluorescence with ischemia compared with control perfusion. Ischemia resulted in significant increases in tissue thiobarbituric acid reactive substance (176 +/- 13 vs. 44 +/- 3 pmol/mg protein for control) and in lung conjugated dienes (0.90 +/- 0.07 vs. 0.48 +/- 0.06 U/mg protein for control), indicating peroxidation of lung lipids. These results indicate that lung ischemia leads to intracellular oxidant generation that can be continuously monitored by surface fluorometry.

scholarly journals SESAMOL ANTAGONIZES ROTENONE-INDUCED CELL DEATH IN SH-SY5Y NEURONAL CELLS

2016 ◽  
Vol 8 (12) ◽  
pp. 72
Author(s):  
Rohini D. ◽  
Vijayalakshmi K.
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Superoxide Dismutase ◽  
Membrane Potential ◽  
Glutathione Peroxidase ◽  
Cell Viability ◽  
Mitochondrial Membrane ◽  
Thiobarbituric Acid ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances

<p><strong>Objective: </strong>To investigate the neuroprotective effect of sesamol against rotenone-induced cell death in SH-SY5Y cells associated with Parkinsonism.</p><p><strong>Methods: </strong>SH-SY5Y cells were maintained in Dulbecco’s modified Eagle’s medium. After differentiation, the cells were incubated with rotenone (20 μM) and sesamol at different concentrations (10-100 μM). Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay. The reactive oxygen species, mitochondrial membrane potential and nuclear morphology were determined by dichlorofluorescein diacetate, rhodamine 123 and 4’, 6-diamidino-2-phenylindole, respectively. Thiobarbituric acid reactive substances, activities of catalase, superoxide dismutase, and glutathione peroxidase and glutathione level were determined by standard assays.</p><p><strong>Results: </strong>Sesamol significantly increased the cell viability and decreased the rotenone-induced cell death in SH-SY5Y cells. Sesamol antagonized rotenone-induced reactive oxygen species generation, loss of mitochondrial membrane potential and nuclear damage. Sesamol also decreased thiobarbituric acid reactive substances level, increased the activities of catalase, superoxide dismutase, glutathione peroxidase and increased the level of glutathione in rotenone-induced cells.</p><p><strong>Conclusion: </strong>The results obtained strongly indicate the promising neuroprotective role of sesamol against rotenone-induced death in SH-SY5Y cells.</p>

scholarly journals Modeling the Kinetic Behavior of Reactive Oxygen Species with Cerium Dioxide Nanoparticles

Biomolecules ◽  
2019 ◽  
Vol 9 (9) ◽  
pp. 447 ◽  
Author(s):  
Kenneth Reed ◽  
Nathan Bush ◽  
Zachary Burns ◽  
Gwendolyn Doherty ◽  
Thomas Foley ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Self Regulation ◽  
Reactive Oxygen ◽  
Molecular Structures ◽  
Time Dependent ◽  
Mathematical Treatment ◽  
Oxygen Species ◽  
Research Activity

The world of medicinal therapies has been historically, and remains to be, dominated by the use of elegant organic molecular structures. Now, a novel medical treatment is emerging based on CeO2 nano-crystals that are discrete clusters of a few hundred atoms. This development is generating a great deal of exciting and promising research activity, as evidenced by this Special Issue of Biomolecules. In this paper, we provide both a steady-state and time-dependent mathematical description of a sequence of reactions: superoxide generation, superoxide dismutase, and hydrogen peroxide catalase and ceria regeneration. This sequence describes the reactive oxygen species (ROS); superoxide, O2–, molecular oxygen, O2, hydroxide ion OH– and hydrogen peroxide, H2O2, interacting with the Ce3+, and Ce4+ surface cations of nanoparticle ceria, CeO2. Particular emphasis is placed on the predicted time-dependent role of the Ce3+/Ce4+ ratio within the crystal. The net reaction is succinctly described as: H2O2 + 2O2– + 2H+ → 2H2O + 2O2. The chemical equations and mathematical treatment appears to align well with several critical in vivo observations such as; direct and specific superoxide dismutase (SOD), ROS control, catalytic regeneration, ceria self-regulation and self-limiting behavior. However, in contrast to experimental observations, the model predicts that the 4+ ceric ion state is the key SOD agent. Future work is suggested based on these calculations.

Hydrogen peroxide-responsive AIE probe for imaging-guided organelle targeting and photodynamic cancer cell ablation

2021 ◽  
Author(s):  
Qian Wu ◽  
Youmei Li ◽  
Ying Li ◽  
Dong Wang ◽  
Ben Zhong Tang
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Cancer Cell ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Cell Ablation ◽  
Selective Monitoring ◽  
Organelle Targeting

Hydrogen peroxide (H2O2), as one kind of key reactive oxygen species (ROS), is mainly produced endogenously primarily in the mitochondria. The selective monitoring of H2O2 in living cells is of...

scholarly journals Upconversion-based nanosystems for fluorescence sensing of pH and H2O2

2021 ◽  
Author(s):  
Chunning Sun ◽  
Michael Gradzielski
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Excitation Energy ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Fluorescence Sensing ◽  
Living Organisms

Hydrogen peroxide (H2O2), a key reactive oxygen species, plays an important role in living organisms, industrial and environmental fields. Here, a non-contact upconversion nanosystem based on the excitation energy attenuation...

Engineering of a novel chimera of superoxide dismutase and Vitreoscilla hemoglobin for rapid detoxification of reactive oxygen species

2010 ◽  
Vol 110 (6) ◽  
pp. 633-637 ◽  
Author(s):  
Chartchalerm Isarankura-Na-Ayudhya ◽  
Sakda Yainoy ◽  
Tanawut Tantimongcolwat ◽  
Leif Bülow ◽  
Virapong Prachayasittikul
Keyword(s):  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Reactive Oxygen ◽  
Vitreoscilla Hemoglobin ◽  
Oxygen Species
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