tert -Butyl hydroperoxide-induced lipid signaling in hepatocytes: involvement of glutathione and free radicals 1 1Abbreviations: [14C]-AA, [14C]-arachidonic acid; DCF, 2′,7′-dichlorofluorescein; DCFDA, 2′,7′-dichlorofluorescin diacetate; DTT, 1,4-dithiothreitol; MDA, malondialdehyde; PLA2, phospholipase A2; ROS, reactive oxygen species; TBARS, thiobarbituric acid reactive substances; and TBHP, tert-butyl hydroperoxide.

2001 ◽  
Vol 62 (6) ◽  
pp. 705-712 ◽  
Author(s):  
César Martı́n ◽  
Rosa Martı́nez ◽  
Rosaura Navarro ◽  
José Ignacio Ruiz-Sanz ◽  
Mercedes Lacort ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Arachidonic Acid ◽  
Thiobarbituric Acid ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Dichlorofluorescin Diacetate ◽  
Ros Reactive Oxygen Species

scholarly journals Protective Effect of Gynura Bicolor Alcohol Extracts on Rat Kidney Epithelial NRK-52E Cells Induced by Tert-Butyl Hydroperoxide

2020 ◽  
Author(s):  
Shu-Ling Hsieh ◽  
Chia-Hsuan Wu ◽  
Jyh-Jye Wang ◽  
Chien-Chun Li ◽  
Chih-Chung Wu
Keyword(s):  
Cell Damage ◽  
Rat Kidney ◽  
Thiobarbituric Acid ◽  
Experimental Models ◽  
Thiobarbituric Acid Reactive Substances ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Tnf Α ◽  
Gynura Bicolor

Abstract Background: Gynura bicolor is a common vegetable in the Asia and has many physiological effects. We aimed to investigate the renoprotective effects of G. bicolor alcohol extracts (GBAEs). Methods: Rat kidney epithelial NRK-52E cell culture and kidney damage induction by tert-butyl hydroperoxide (t-BHP) were used as experimental models. In this study, the antioxidative and anti-inflammatory activities, the levels of apoptosis and apoptosis-related molecules were analyzed in NRK-52E cells following t-BHP-induced injury. Results: The results showed the levels of thiobarbituric acid reactive substances (TBARS) and reactive oxygen species (ROS) decreased by levels by 61-71% and 41-59%, respectively, in NRK-52E cells treated with 0.5, 1, or 5 mg/ml GBAE combined with t-BHP compared with t-BHP-induced cells (p<0.05). The GSH levels and GSH-to-GSSG ratio were increased in NRK-52E cells treated with 0.5, 1, and 5 mg/ml GBAE combined with t-BHP compared to t-BHT-induced cells (p<0.05). The nitrite (NO), prostaglandin E2 (PGE2), IL-6, and TNF-α levels of NRK-52E cells treated with 0.5, 1, and 5 mg/ml GBAE combined with t-BHP were decreased compared with t-BHP-induced cells (p<0.05). In addition, Bcl-2 levels were increased after treatment with 5 mg/ml GBAE combined with t-BHP, and the levels of Bax and poly (ADP-ribose) polymerase (PARP) and the apoptosis percentage were decreased after treatment with 1 or 5 mg/ml GBAE combined with t-BHP induction compared to after t-BHP induction alone (p<0.05). Conclusions: These data showed that 5 mg/ml GBAE can protect against kidney cell damage induced by t-BHP. The mechanism involves reducing cell damage and apoptosis through decreasing oxidative stress and inflammation.

scholarly journals Baicalein protects tert-butyl hydroperoxide-induced hepatotoxicity dependent of reactive oxygen species removal

2017 ◽  
Vol 16 (6) ◽  
pp. 8392-8398
Author(s):  
Ya-Fang Wang ◽  
Zheng-Hai Tang ◽  
Ting Li ◽  
Xiao-Huang Xu ◽  
Xiuping Chen ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Butyl Hydroperoxide ◽  
Tert Butyl ◽  
Tert Butyl Hydroperoxide ◽  
Species Removal

scholarly journals Effects of [Cu(2-MeSNic)2(MeNia)2(H2O)2]·H2O and [Cu(2-MeSNic)2(H2O)]2 on Candida albicans

2015 ◽  
Vol 8 (1) ◽  
pp. 39-43
Author(s):  
Barbora Kaliňáková ◽  
Daniela Hudecová ◽  
Peter Segľa ◽  
Martina Palicová ◽  
Jozef Švorec
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Candida Albicans ◽  
Copper Complexes ◽  
Membrane Damage ◽  
Thiobarbituric Acid ◽  
Intracellular Reactive Oxygen Species ◽  
Oxygen Species ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hoechst 33342

Abstract Probable mode of action of new copper complexes of 2-methylthionicotinate (2-MeSNic) of composition [Cu(2-MeSNic)2(H2O)2] and [Cu(2-MeSNic)2(MeNia)2(H2O)2]·H2O (where MeNia is N-methylnicotinamide) is described. Both partial growth inhibition of Candida albicans (IC50 ≥ 1.78 mmol·L−1, MIC ≥ 2.5 mmol·L−1) and leak of proteins into the extracellular space (more than 80 %) were observed in the presence of these copper complexes. The membrane damage was detected by staining with Hoechst 33342, propidium iodide and methylene blue. Ascorbic acid potentiated antifungal activity of copper complexes approximately seven-fold and induced the oxidative stress, respectively. The production of intracellular reactive oxygen species was visualized by dichlorofluorescein. Thiobarbituric acid-reactive substances were formed as a by-product of lipid peroxidation.

Induction ofPseudomonas aeruginosafhpandfhpRby reactive oxygen species

2009 ◽  
Vol 55 (6) ◽  
pp. 657-663 ◽  
Author(s):  
Taija S. Koskenkorva-Frank ◽  
Pauli T. Kallio
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Promoter Activity ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Low Oxygen ◽  
Butyl Hydroperoxide ◽  
Tert Butyl Hydroperoxide ◽  
Oxygen Conditions

In Pseudomonas aeruginosa , flavohemoglobin (Fhp) and its cognate regulator FhpR (PA2665) form a protective regulatory circuit, which responds to reactive nitrogen species and is also capable of protecting cells against nitrosative stress. Recently, it has been shown that the expression of the fhp promoter is regulated not only by FhpR, but also by two new regulators, PA0779 and PA3697. It has also been suggested that the bacterial flavohemoglobins (flavoHbs) could play a crucial role in the protection of cells against reactive oxygen species (ROS). Therefore, the role and function of the Fhp/FhpR system during oxidative stress were studied by assessing the viability and membrane integrity of P. aeruginosa cells and by analyzing the promoter activities of fhp and fhpR upon exposure to paraquat, hydrogen peroxide, and tert-butyl hydroperoxide, under both aerobic and low-oxygen conditions. The results showed that under aerobic conditions, both fhp and fhpR promoters are induced by ROS generated by the stressors. Thus, the Fhp/FhpR system is implicated in the oxidative stress response. ROS-induced fhp promoter activity was dependent on FhpR, PA0779, and PA3697 regulators. Tert-butyl hydroperoxide-induced fhpR promoter activity was found to be highly repressed by PA0779, and FhpR showed negative autoregulation of its own promoter. Under low-oxygen conditions, the activity of the fhp promoter was not inducible by ROS, but fhpR promoter activity was induced by paraquat, and hydrogen peroxide was repressed in both cases by the regulators PA0779 and PA3697.

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