The platelet intracellular calcium response to serotonin and thrombin is augmented in patients with panic disorder: A pilot study

SummaryHuman platelets are thought to possess at least two subtypes of purinoceptor, one of which, coupled to G-proteins, could be the P2Y1 receptor (Léon et al. 1997). However, it has been suggested that the unique rapid calcium influx induced by ADP in platelets could involve P2X1 ionotropic receptors (MacKenzie et al. 1996) and the aim of this study was thus to investigate the presence of P2X purinoceptors in platelets and megakaryoblastic cells. Using PCR experiments, we found P2X1 mRNA to be present in human platelets and megakaryoblastic cell lines. In platelets, the selective P2X1 agonist αβMeATP induced a rise in intracellular calcium only in the presence of external calcium and this effect was antagonized by suramin and PPADS. Repeated addition of a�MeATP desensitized the P2X1 purinoceptor but only slightly affected the ADP response, while no calcium response to αβMeATP was observed in megakaryoblastic cells. These results support the existence of functional P2X1 purinoceptors on human platelets and the presence of P2X1 transcripts in megakaryoblastic cell lines.

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Interleukin-6 selectively enhances the intracellular calcium response to NMDA in developing CNS neurons

Journal of Neuroscience ◽

10.1523/jneurosci.15-10-06688.1995 ◽

1995 ◽

Vol 15 (10) ◽

pp. 6688-6699 ◽

Cited By ~ 60

Author(s):

Z Qiu ◽

KL Parsons ◽

DL Gruol

Keyword(s):

Intracellular Calcium ◽

Interleukin 6 ◽

Calcium Response ◽

Cns Neurons

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Intracellular calcium response is reduced in CD4+ lymphocytes in Alzheimer's disease and in older persons with down's syndrome

Neurobiology of Aging ◽

10.1016/0197-4580(93)90094-r ◽

1993 ◽

Vol 14 (2) ◽

pp. 177-185 ◽

Cited By ~ 21

Author(s):

Angelika Grossmann ◽

Walter A. Kukull ◽

John C. Jinneman ◽

Thomas D. Bird ◽

Enrique C. Villacres ◽

...

Keyword(s):

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Intracellular Calcium ◽

Older Persons ◽

Down's Syndrome ◽

Down’S Syndrome ◽

Calcium Response ◽

Cd4 Lymphocytes

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Computer-aided self-help for phobia/panic via internet at home: A pilot study

The British Journal of Psychiatry ◽

10.1192/bjp.184.5.448 ◽

2004 ◽

Vol 184 (5) ◽

pp. 448-449 ◽

Cited By ~ 45

Author(s):

Mark Kenwright ◽

Isaac M. Marks ◽

Lina Gega ◽

David Mataix-Cols

Keyword(s):

Pilot Study ◽

Panic Disorder ◽

Open Study ◽

The Internet ◽

Face To Face ◽

Self Help ◽

Computer Aided ◽

Therapist Support ◽

At Home ◽

Help System

SummaryIn an open study, ten people with phobia or panic disorder who could not travel repeatedly to a therapist accessed a computer-aided exposure self-help system (Fear Fighter) at home on the internet with brief therapist support by telephone. They improved significantly, and their outcome and satisfaction resembled those in patients with similar disorders who used Fear Fighter in clinics with brief face-to-face therapist support.

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Multiple Receptor Interactions Trigger Release of Membrane and Intracellular Calcium Stores Critical for Herpes Simplex Virus Entry

Molecular Biology of the Cell ◽

10.1091/mbc.e07-01-0062 ◽

2007 ◽

Vol 18 (8) ◽

pp. 3119-3130 ◽

Cited By ~ 45

Author(s):

Natalia Cheshenko ◽

Wen Liu ◽

Lisa M. Satlin ◽

Betsy C. Herold

Keyword(s):

Herpes Simplex ◽

Intracellular Calcium ◽

Viral Entry ◽

Calcium Stores ◽

Calcium Response ◽

Herpes Simplex Viruses ◽

Intracellular Calcium Stores ◽

Viral Binding ◽

Glycoprotein H ◽

Simplex Virus

Herpes simplex viruses (HSV) harness cellular calcium signaling pathways to facilitate viral entry. Confocal microscopy and small interfering RNA (siRNA) were used to identify the source of the calcium and to dissect the requisite viral–cell interactions. Binding of HSV to human epithelial cells induced no calcium response, but shifting the cells to temperatures permissive for penetration triggered increases in plasma membrane calcium followed by a global release of intracellular calcium. Transfection with siRNA targeting the proteoglycan syndecan-2 blocked viral binding and abrogated any calcium response. Transfection with siRNA targeting nectin-1, a glycoprotein D receptor, also prevented both membrane and intracellular calcium responses. In contrast, the membrane response was preserved after transfection with siRNA targeting integrinαv, a novel glycoprotein H receptor. The membrane response, however, was not sufficient for viral entry, which required interactions with integrinαv and release of inositol-triphosphate receptor-dependent intracellular calcium stores. Thus, calcium plays a critical, complex role in HSV entry.

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