A Naturally Occurring Latent Bedsonia Infection in Guinea-Pigs

Osteoarthritis (OA) is an increasingly prevalent disease affecting synovial joints, which includes joint degeneration, inflammation, and joint pain. The activation of nucleotide-binding and oligomerization domain-like receptor containing protein 3 (NLRP3) could promote synovial inflammation. Previous studies have shown that electroacupuncture (EA) has potential anti-inflammatory effect. However, the effect of EA treatment on OA remains unclear. The aim of this study was to investigate the effect of applied EA on OA and joint pain and its relationship with NLRP3 inflammasome. The Hartley guinea pigs with naturally occurring OA at age 18 months were chosen as the OA model and treated with EA for 4 weeks. Mechanical allodynia was quantified by using von Frey filaments. The expression of NLRP3 inflammasome and the downstream proinflammatory factors in the cartilage tissue were quantified. Our results showed that EA treatment significantly reduces mechanical allodynia, improves the articular cartilage structure, and decreases the fibrillation on the cartilage surface in guinea pigs with spontaneous osteoarthritis. Moreover, we also found that EA treatment attenuates the NLRP3 inflammasome activation and suppresses the protein expression levels of caspase-1 and IL-1β in the cartilage tissue. Our findings suggest that EA treatment attenuates OA and joint pain by suppressing NLRP3 inflammasome activation and support further investigation of the potential therapeutic tactics.

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The sensitizing capacity of naturally occurring quinones.?Experimental studies in guinea pigs

Archives of Dermatological Research ◽

10.1007/bf00582866 ◽

1977 ◽

Vol 258 (1) ◽

pp. 41-52 ◽

Cited By ~ 32

Author(s):

K. H. Schulz ◽

I. Garbe ◽

B. M. Hausen ◽

M. H. Simatupang

Keyword(s):

Guinea Pigs ◽

Experimental Studies ◽

Naturally Occurring

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Naturally occurring human anti-band 3 autoantibodies accelerate clearance of erythrocytes in guinea pigs

Blood ◽

10.1182/blood.v85.7.1920.bloodjournal8571920 ◽

1995 ◽

Vol 85 (7) ◽

pp. 1920-1928 ◽

Cited By ~ 18

Author(s):

U Giger ◽

B Sticher ◽

R Naef ◽

R Burger ◽

HU Lutz

Keyword(s):

Guinea Pigs ◽

Band 3 ◽

Complement C3 ◽

Erythrocyte Membranes ◽

Dependent Manner ◽

Band 3 Protein ◽

Naturally Occurring ◽

Dose Dependent

A variety of naturally occurring autoantibodies (NOAs) have been found in sera of animals and humans. Although their specific homeostatic role in the clearance of altered or senescent cells has been proposed and in vitro studies support such functions, in vivo evidence has been lacking. We studied the effect of affinity-purified human anti-band 3 NOA on the survival of untreated and diamide-treated erythrocytes in normal and complement C3-deficient guinea pigs. In vitro exposure to diamide, an oxidative agent, severely reduced the erythrocyte deformability and increased the amount of high-molecular-weight forms of band 3 protein and band 3-hemoglobin adducts in erythrocyte membranes, thereby markedly shortening the survival of these cells in vivo. Human anti-band 3 NOA bound in a dose-dependent manner to erythrocytes, and binding increased with exposure to diamide. In normal guinea pigs anti-band 3 NOA significantly accelerated the clearance of erythrocytes that were mildly damaged by iodine surface labeling and of those that were further oxidized by diamide. However, the anti-band 3 effect was transient and small. In contrast, anti-band 3 NOA did not significantly alter erythrocyte survival in functionally C3-deficient guinea pigs, thereby supporting the C3b requirement for anti-band 3 NOA activity. On the other hand, a pretreatment of animals with purified human band 3 protein slowed down the clearance of erythrocytes incubated with IgG depleted of anti-band 3 NOA. These results provide the first in vivo evidence of a role for anti-band 3 NOA in the clearance of erythrocytes.

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Effects of Dopaminergic Agents on Progression of Naturally Occurring Myopia in Albino Guinea Pigs (Cavia porcellus)

Investigative Opthalmology & Visual Science ◽

10.1167/iovs.14-14294 ◽

2014 ◽

Vol 55 (11) ◽

pp. 7508 ◽

Cited By ~ 27

Author(s):

Liqin Jiang ◽

Keli Long ◽

Frank Schaeffel ◽

Xiangtian Zhou ◽

Yibo Zheng ◽

...

Keyword(s):

Guinea Pigs ◽

Cavia Porcellus ◽

Naturally Occurring ◽

Dopaminergic Agents

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Resveratrol induces vasorelaxation of mesenteric and uterine arteries from female guinea-pigs

Clinical Science ◽

10.1042/cs0980537 ◽

2000 ◽

Vol 98 (5) ◽

pp. 537-543 ◽

Cited By ~ 34

Author(s):

Ebrahim K. NADERALI ◽

Patrick J. DOYLE ◽

Gareth WILLIAMS

Keyword(s):

Methyl Ester ◽

Blood Vessels ◽

Guinea Pigs ◽

Time Course ◽

Oestrous Cycle ◽

Mesenteric Arteries ◽

Resistance Arteries ◽

Uterine Arteries ◽

Naturally Occurring ◽

Different Types

Naturally occurring hydroxystilbenes have been shown to induce vasorelaxation. Here, we studied the mechanism of resveratrol-induced vasorelaxation in different types of blood vessels, namely mesenteric (resistance) and main uterine (conductance) arteries, from female guinea-pigs on day 7 and day 15 of the oestrous cycle. Resveratrol (5–70 µmol/l) induced concentration-dependent relaxation of both mesenteric and uterine arteries preconstricted with either noradrenaline (NA; 10 µmol/l) or KCl (125 mmol/l). Resveratrol was 2-fold more potent in inducing relaxation of mesenteric arteries than of uterine arteries. Its effects on uterine arteries from both day-7 and day-15 guinea-pigs were similar, irrespective of the constrictor used, but it was significantly (P < 0.01) more potent in inducing relaxation of mesenteric arteries contracted with NA compared with those constricted with KCl. In day-7 arteries precontracted with NA, NG-nitro-l-arginine methyl ester (l-NAME; 10 µmol/l) had no effects on the time course of resveratrol-induced vasorelaxation in either mesenteric or uterine arteries. However, indomethacin (50 µmol/l) significantly (P < 0.05) potentiated resveratrol's effect on mesenteric, but not uterine, arteries. Indomethacin had no effect on resveratrol-induced vasorelaxation of arteries contracted with KCl, whereas l-NAME significantly (P < 0.05) reduced the effects of resveratrol on uterine, but not on mesenteric, arteries. In day-15 arteries, l-NAME significantly (P < 0.01) attenuated the effects of resveratrol on mesenteric arteries contracted with NA. Indomethacin had no effect on resveratrol activity. This study indicates that: (a) the effect of resveratrol on resistance arteries is greater than that on conductance arteries; (b) the effects of resveratrol are not mediated via prostanoids, but NO may play a role; and (c) the stage of the oestrous cycle has no influence on resveratrol-induced vasorelaxation.

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The sensitizing capacity of naturally occurring quinones experimental studies in guinea pigs

Archives of Dermatological Research ◽

10.1007/bf00412654 ◽

1979 ◽

Vol 264 (3) ◽

pp. 275-286 ◽

Cited By ~ 49

Author(s):

K. H. Schulz ◽

I. Garbe ◽

B. M. Hausen ◽

M. H. Simatupang

Keyword(s):

Guinea Pigs ◽

Experimental Studies ◽

Naturally Occurring

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PHASE VARIATION OF THE NINE MILE AND OTHER STRAINS OF RICKETTSIA BURNETI

Canadian Journal of Microbiology ◽

10.1139/m56-036 ◽

1956 ◽

Vol 2 (3) ◽

pp. 310-321 ◽

Cited By ~ 89

Author(s):

M. G. P. Stoker ◽

P. Fiset

Keyword(s):

Guinea Pigs ◽

Complement Fixation ◽

Q Fever ◽

Early Stage ◽

Phase 1 ◽

Phase Variation ◽

Yolk Sac ◽

Phase 2 ◽

Naturally Occurring

The classical Nine Mile strain of Rickettsia burneti, which had been maintained in guinea pigs since first isolation, was adapted to growth in yolk sacs. Despite abundant rickettsiae, antigens prepared from each of the first seven yolk sac passages failed to react in complement fixation tests with homologous Q fever antisera. The antisera did react, however, with antigens prepared from the eighth and subsequent passages. This change in behavior during egg adaptation, which resembles that found previously with freshly isolated strains, is termed "phase variation". Rickettsiae in an early stage of egg adaptation which fail to fix complement with antisera are called Phase 1; after further egg adaptation and the development of ability to react with antiserum, they are termed Phase 2. Strains in Phase 2 rapidly reverted to Phase 1 after inoculation into guinea pigs, mice, or hamsters, and naturally occurring strains from a sheep's placenta, cows' milk, and ticks were also found to be in Phase 1. It is possible that all strains used for preparing stock complement fixing antigens are laboratory variants in Phase 2 obtained through multiple yolk sac passages.

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Naturally Occurring Virus-associated Respiratory Disease in Two Guinea Pigs

Veterinary Pathology ◽

10.1177/030098588302000411 ◽

1983 ◽

Vol 20 (4) ◽

pp. 488-491 ◽

Cited By ~ 12

Author(s):

L. H. Brennecke ◽

T. M. Dreier ◽

W. S. Stokes

Keyword(s):

Respiratory Disease ◽

Guinea Pigs ◽

Naturally Occurring

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