37. Intracellular water content of Saccharomyces cerevisiae during freezing at constant cooling rates

Cryobiology ◽  
1973 ◽  
Vol 10 (6) ◽  
pp. 517-518 ◽  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Water Content ◽  
Intracellular Water ◽  
Cooling Rates

Water content of rat adipose tissue and isolated adipocytes in relation to cell size

1976 ◽  
Vol 231 (5) ◽  
pp. 1568-1572 ◽  
Author(s):  
M DiGirolamo ◽  
JL Owens
Keyword(s):  
Adipose Tissue ◽  
Water Content ◽  
Cell Volume ◽  
Cell Size ◽  
Intracellular Water ◽  
Male Rats ◽  
Fat Cells ◽  
Fat Cell ◽  
Tissue Water ◽  
Adipose Mass

Epididymal adipose tissue composition and adipocyte water content were studied in male rats during growth and development of spontaneous obesity. The data show that a highly significant positive correlation exists between fat-cell volume and intracellular water space (IWS) (r=.967, P less than .001). Intracellular water, expressed as picoliters per fat cell, varied from 1.5-2 in small fat cells (mean vol, 30-50 pl) to 9-10 in large cells (800-1,000 pl). When expressed as percent of fat-cell volume, IWS varied from 5-7% in the small fat cells to 1-1.3% in the large ones. Total adipose tissue water continued to increase with increasing adipose mass. Similarly, total adipocyte water increased with enlarging cell size and tissue mass. The contribution of total adipocyte water (as contrasted to that of nonadipocyte water) to total tissue water, however, was found to be limited (less than 23%) and to decline progressively with adipose mass expansion.

Effects of drought on water content and photosynthetic parameters in potato plants expressing the trehalose-6-phosphate synthase gene of Saccharomyces cerevisiae

Planta ◽  
2007 ◽  
Vol 227 (2) ◽  
pp. 299-308 ◽  
Author(s):  
Ibolya Stiller ◽  
Sándor Dulai ◽  
Mihály Kondrák ◽  
Réka Tarnai ◽  
László Szabó ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Water Content ◽  
Photosynthetic Parameters ◽  
Potato Plants ◽  
Effects Of Drought ◽  
Phosphate Synthase

scholarly journals A multiscale approach to estimate the cellular diffusivity during food drying

2021 ◽  
Author(s):  
Zachary G Welsh
Keyword(s):  
Water Content ◽  
Effective Diffusivity ◽  
Theoretical Models ◽  
Intracellular Water ◽  
Cellular Heterogeneity ◽  
Multiscale Approach ◽  
Multiscale Models ◽  
Drying Model ◽  
Cellular Water ◽  
Multiscale Homogenization

Theoretical models for food drying commonly utilize an effective diffusivity solved through curve fitting based on experimental data. This creates models with limited predictive capabilities. Multiscale modeling is one approach which can help transition to a more physics-based model minimizing the empirical information required while improving a model’s predictive capabilities. However, to enable an accurate scaling operation, multiscale models require diffusivity at a fine scale (microscale). Measuring these properties is experimentally costly and time consuming as they are often temperature and/or moisture dependent. This research conducts an inverse analysis on a multiscale homogenization food drying model to deduce the temporal diffusivity of intracellular water. A representation of the real cellular water breakdown was considered and appropriate assumptions to represent its cellular heterogeneity, in relation to time, were investigated. The work uncovered that a linear decrease in intracellular water content could be assumed and thus a function for its diffusivity was developed. The proposed function is in terms of sample temperature and intracellular water content opening the possibilities to be applied to various food materials.

scholarly journals Platelet Water Content is Decreased by Gel-Filtration

1977 ◽  
Author(s):  
J.S. Wiley ◽  
G. Wray ◽  
I.A. Cooper
Keyword(s):  
Water Content ◽  
Gel Filtration ◽  
Normal Subjects ◽  
Intracellular Water ◽  
Total Water ◽  
Initial Water ◽  
Mean Cell Volume ◽  
Cell Pellet ◽  
The Mean ◽  
Water Space

One approach to platelet sizing is to measure the intracellular water space of platelets with 3H-H2O since the % water content of platelets remains constant in states with different platelet sizes. Fresh citrated blood was centrifuged for 10 min at 150 'g' to obtain PRP. Aliquots of PRP were briefly incubated with either 3H-H2O or 14C-sucrose then layered over 0.3 ml dibutylphthalate and spun 4 min at 8000 'g'. The cell pellet was solubilized and counted to enable spaces to be calculated. The extracellular (sucrose) space was subtracted from the total water space of the pellet to give a mean intracellular water space of 0.56 ± 0.12 μ1/108 platelets (n =19). Assuming a water content of 7 5% and a density of 1.04, the mean platelet volume for normal subjects is 7.2 fl. Gel-filtration of platelets (GFP) on Sepharose-2B reduced their mean water space by 0.12 μl/108 platelets. However the amount of shrinkage on gel-filtration depended on the initial water space of the platelets in PRP and there was a linear relation between these two variables (r = 0.82). Shrinkage was 40% for an initial platelet water space of 0.70 μl/108 platelets but there was almost no shrinkage below a water space of 0.40 μl/108 platelets. Recovery of platelets from each column averaged 8 0% and showed no relation to the reduction in the mean cell water space. The lower water space of GFP may indicate a reduction in mean cell volume due to gel-filtration.

scholarly journals Intracellular Water Content in Lean Mass as an Indicator of Muscle Quality in an Older Obese Population

2020 ◽  
Vol 9 (5) ◽  
pp. 1580
Author(s):  
Mateu Serra-Prat ◽  
Isabel Lorenzo ◽  
Mònica Papiol ◽  
Elisabet Palomera ◽  
Maria Bartolomé ◽  
...  
Keyword(s):  
Muscle Strength ◽  
Water Content ◽  
Muscle Mass ◽  
Lean Mass ◽  
Gait Speed ◽  
Bioelectrical Impedance ◽  
Intracellular Water ◽  
Muscle Quality ◽  
Timed Up And Go ◽  
Cross Sectional

Background: In aged populations, muscle strength depends more on muscle quality than on muscle quantity, while all three are criteria for the diagnosis of sarcopenia. Intracellular water content (ICW) in lean mass (LM) has been proposed as an indicator of muscle quality related to muscle strength in older people. Objectives: To evaluate the relationship between the ICW/LM ratio, muscle strength and indicators of functional performance in obese older adults, and to assess the value of the ICW/LM ratio as an indicator of muscle quality. Methodology: Design: cross-sectional study. Population: persons aged 65–75 years with a body mass index of 30–39 kg/m2. ICW and LM were estimated by bioelectrical impedance. Hand grip, gait speed, unipedal stance test, timed up-and-go (TUG) test, Barthel score and frailty (Fried criteria) were assessed. Sarcopenia was established according to EWGSOP2 criteria. Results: Recruited were 305 subjects (66% women), mean age 68 years. The ICW/LM ratio correlated with the TUG test, gait speed and grip strength, and was also associated with sex, the unipedal stance test and frailty. Independently of age, sex and muscle mass, the ICW/LM ratio was related with gait speed, the TUG test and unipedal stance capacity. One person (0.3%) had sarcopenia defined as low muscle strength and low muscle mass, while 25 people (8.2%) had sarcopenia defined as low muscle strength and poor muscle quality (ICW/LM). With this last definition, sarcopenia was related to frailty, gait speed and the TUG test. Conclusions: ICW content in LM could be a useful muscle quality indicator for defining sarcopenia. However, more studies are required to confirm our findings for other populations.

Volumetric changes in Saccharomyces cerevisiae during freezing at constant cooling rates

Cryobiology ◽  
1972 ◽  
Vol 9 (4) ◽  
pp. 324 ◽  
Author(s):  
M. Ushiyama ◽  
E. Cravalho ◽  
K. Diller
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cooling Rates

scholarly journals Fractional extracellular space and fractional water content of various rat tissues at different extracellular pH values and in uremia

1979 ◽  
Vol 13 (2) ◽  
pp. 171-174 ◽  
Author(s):  
K. F. Rothe
Keyword(s):  
Water Content ◽  
Extracellular Space ◽  
Brain Oedema ◽  
Extracellular Ph ◽  
Intracellular Water ◽  
Rat Tissues ◽  
Cellular Space ◽  
Ph Values

Summary At different extracellular pH values fractional extra-cellular space (calculated from the distribution of 3H inulin) and fractional water content showed no significant differences. 72 h after nephrectomy both variables increased significantly. An exception to this was brain, where fractional extracellular space decreased and total intracellular water increased as a sign of brain oedema.

scholarly journals A bioimpedance analysis of head-and-neck cancer patients undergoing radiotherapy

2018 ◽  
Vol 25 (3) ◽  
pp. 193 ◽  
Author(s):  
K. Kohli ◽  
R. Corns ◽  
K. Vinnakota ◽  
P. Steiner ◽  
C. Elith ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Head And Neck ◽  
Water Content ◽  
Cancer Patients ◽  
Neck Cancer ◽  
Membrane Integrity ◽  
Bioelectrical Impedance ◽  
Impedance Analysis ◽  
Intracellular Water ◽  
Cell Membrane Integrity

Malnutrition is a frequent manifestation in patients with head-and-neck cancer undergoing radiation therapy and a major contributor to morbidity and mortality. Thus, body composition is an important component of an overall evaluation of nutrition in cancer patients. Malnutrition is characterized by weight loss, loss of muscle mass, changes in cell membrane integrity, and alterations in fluid balance. Bioelectrical impedance analysis is a method to analyze body composition and includes parameters such as intracellular water content, extracellular water content, and cell membrane integrity in the form of a phase angle (Φ). Bioelectrical impedance analysis has consistently been shown to have prognostic value with respect to mortality and morbidity in patients undergoing chemotherapy. The goal of the present study was to evaluate the relationship between Φ, time, intracellular water content, and weight for head-and-neck cancer patients undergoing radiotherapy. The results demonstrate that Φ decreases with time and increases with intracellular water content and weight.

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