An intact zona pellucida is not necessary for successful mouse embryo cryopreservation**Presented in part at the Sixth World Congress of In Vitro Fertilization, Jerusalum, Israel, April 2 to 7, 1989.††Supported in part by grant HD25136 from the National Institutes of Health, Bethesda, Maryland.

1992 ◽  
Vol 57 (3) ◽  
pp. 677-681 ◽  
Author(s):  
G. John Garrisi ◽  
Beth E. Talansky ◽  
Valdi Sapira ◽  
Jon W. Gordon ◽  
Daniel Navot
Keyword(s):  
In Vitro Fertilization ◽  
Mouse Embryo ◽  
Zona Pellucida ◽  
National Institutes Of Health ◽  
Embryo Cryopreservation ◽  
World Congress ◽  
Vitro Fertilization

Differences between antigenic determinants of pig and cat zona pellucida proteins

Reproduction ◽  
2000 ◽  
pp. 15-23 ◽  
Author(s):  
K Jewgenow ◽  
M Rohleder ◽  
I Wegner
Keyword(s):  
In Vitro Fertilization ◽  
Zona Pellucida ◽  
Antigenic Determinants ◽  
Vitro Fertilization ◽  
Contraceptive Vaccine ◽  
Sperm Binding ◽  
Dependent Inhibition ◽  
Dose Dependent Inhibition ◽  
Dose Dependent

Despite many efforts, the control of reproduction in feral cat populations is still a problem in urban regions around the world. Immunocontraception is a promising approach; thus the present study examined the suitability of the widely used pig zona pellucida proteins (pZP) for contraception in feral domestic cats. Purified zona pellucida proteins obtained from pig and cat ovaries were used to produce highly specific antisera in rabbits. Antibodies against pZP raised in rabbits or lions were not effective inhibitors of either in vitro sperm binding (cat spermatozoa to cat oocytes) or in vitro fertilization in cats, whereas antibodies against feline zona pellucida proteins (fZP) raised in rabbits showed a dose-dependent inhibition of in vitro fertilization. Immunoelectrophoresis, ELISA and immunohistology of ovaries confirmed these results, showing crossreactivity of anti-fZP sera to fZP and to a lesser extent to pZP, but no interaction of anti-pZP sera with fZP. It is concluded that cat and pig zonae pellucidae express a very small number of shared antigenic determinants, making the use of pZP vaccine in cats questionable. A contraceptive vaccine based on feline zona pellucida determinants will be a better choice for the control of reproduction in feral cats if immunogenity can be achieved.

A booklet on embryo cryopreservation for couples undergoing in vitro fertilization: exploring its utility

2013 ◽  
Vol 100 (3) ◽  
pp. S409
Author(s):  
M.S. Christianson ◽  
L. Londra ◽  
S. Seifert ◽  
H. Wu ◽  
A.M. Khafagy ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Cryopreservation ◽  
Vitro Fertilization

scholarly journals Differential influence of ampullary and isthmic derived epithelial cells on zona pellucida hardening and in vitro fertilization in ovine

2016 ◽  
Vol 16 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Navid Dadashpour Davachi ◽  
Ahmad Zare Shahneh ◽  
Hamid Kohram ◽  
Mahdi Zhandi ◽  
Helia Shamsi ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Epithelial Cells ◽  
Zona Pellucida ◽  
Vitro Fertilization
Sign in / Sign up

Export Citation Format

Share Document