The effects of dieldrin on the subcellular structure and function of mammalian liver cells

1972 ◽  
Vol 10 (3) ◽  
pp. 311-332 ◽  
Author(s):  
A.S. Wright ◽  
D. Potter ◽  
M.F. Wooder ◽  
C. Donninger ◽  
R.D. Greenland
Parasitology ◽  
1934 ◽  
Vol 26 (2) ◽  
pp. 176-248 ◽  
Author(s):  
G. S. Graham-Smith

A detailed description of the musculature in the different regions of the alimentary canal of Calliphora erythrocephala is given, and an account of the structure and function of the crop, proventriculus, ducts of the Malpighian tubes, rectal valve and rectal papillae. It has been shown by dissections and experiments that a system of channels exists in the rectal papillae through which the body fluid probably circulates, and it is suggested that the very large cells may have functions resembling those of liver cells.


2007 ◽  
Vol 0 (0) ◽  
pp. 070816212604002-??? ◽  
Author(s):  
Maria Bokhari ◽  
Ross J. Carnachan ◽  
Neil R. Cameron ◽  
Stefan A. Przyborski

1969 ◽  
Vol 41 (2) ◽  
pp. 477-493 ◽  
Author(s):  
Bernard Tandler ◽  
Robert A. Erlandson ◽  
Archie L. Smith ◽  
Ernest L. Wynder

Mice which had been on a riboflavin-free diet for 6–8 wk were given daily intraperitoneal injections of riboflavin. The hepatic mitochondria, which in the deficient animals were greatly enlarged, were restored to normal dimensions within 3 days. Normalization of the mitochondrial population was brought about by division of the giant organelles. Dividing mitochondria were characterized by a membranous partition separating the inner compartment into two distinct chambers. Such organelles showed varying degrees of pinching at the level of the partition. The most common site of partition formation was at the base of a small mitochondrial bud. During the 1st day of recovery, dividing mitochondria were so common that they could be easily found in mitochondrial pellets. Injection of riboflavin into normally nourished mice also produced an apparent increase in the frequency of dividing mitochondria in the liver cells.


1970 ◽  
Vol 117 (3) ◽  
pp. 63P-64P ◽  
Author(s):  
G. Ragnotti ◽  
F. Cajone

Author(s):  
Peter Sterling

The synaptic connections in cat retina that link photoreceptors to ganglion cells have been analyzed quantitatively. Our approach has been to prepare serial, ultrathin sections and photograph en montage at low magnification (˜2000X) in the electron microscope. Six series, 100-300 sections long, have been prepared over the last decade. They derive from different cats but always from the same region of retina, about one degree from the center of the visual axis. The material has been analyzed by reconstructing adjacent neurons in each array and then identifying systematically the synaptic connections between arrays. Most reconstructions were done manually by tracing the outlines of processes in successive sections onto acetate sheets aligned on a cartoonist's jig. The tracings were then digitized, stacked by computer, and printed with the hidden lines removed. The results have provided rather than the usual one-dimensional account of pathways, a three-dimensional account of circuits. From this has emerged insight into the functional architecture.


Author(s):  
K.E. Krizan ◽  
J.E. Laffoon ◽  
M.J. Buckley

With increase use of tissue-integrated prostheses in recent years it is a goal to understand what is happening at the interface between haversion bone and bulk metal. This study uses electron microscopy (EM) techniques to establish parameters for osseointegration (structure and function between bone and nonload-carrying implants) in an animal model. In the past the interface has been evaluated extensively with light microscopy methods. Today researchers are using the EM for ultrastructural studies of the bone tissue and implant responses to an in vivo environment. Under general anesthesia nine adult mongrel dogs received three Brånemark (Nobelpharma) 3.75 × 7 mm titanium implants surgical placed in their left zygomatic arch. After a one year healing period the animals were injected with a routine bone marker (oxytetracycline), euthanized and perfused via aortic cannulation with 3% glutaraldehyde in 0.1M cacodylate buffer pH 7.2. Implants were retrieved en bloc, harvest radiographs made (Fig. 1), and routinely embedded in plastic. Tissue and implants were cut into 300 micron thick wafers, longitudinally to the implant with an Isomet saw and diamond wafering blade [Beuhler] until the center of the implant was reached.


Author(s):  
Robert L. Ochs

By conventional electron microscopy, the formed elements of the nuclear interior include the nucleolus, chromatin, interchromatin granules, perichromatin granules, perichromatin fibrils, and various types of nuclear bodies (Figs. 1a-c). Of these structures, all have been reasonably well characterized structurally and functionally except for nuclear bodies. The most common types of nuclear bodies are simple nuclear bodies and coiled bodies (Figs. 1a,c). Since nuclear bodies are small in size (0.2-1.0 μm in diameter) and infrequent in number, they are often overlooked or simply not observed in any random thin section. The rat liver hepatocyte in Fig. 1b is a case in point. Historically, nuclear bodies are more prominent in hyperactive cells, they often occur in proximity to nucleoli (Fig. 1c), and sometimes they are observed to “bud off” from the nucleolar surface.


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