Effects of homeobox transcription factors CDX2 and PDX1 on the calbindin-D9K promoter

2000 ◽  
Vol 118 (4) ◽  
pp. A287
Author(s):  
Natalie F. Barley ◽  
Stephen Legon ◽  
Julian Rf Walters
Keyword(s):  
Transcription Factors ◽  
Calbindin D9k ◽  
Homeobox Transcription Factors

scholarly journals Homeobox Transcription Factors Are Required for Conidiation and Appressorium Development in the Rice Blast Fungus Magnaporthe oryzae

PLoS Genetics ◽  
2009 ◽  
Vol 5 (12) ◽  
pp. e1000757 ◽  
Author(s):  
Seryun Kim ◽  
Sook-Young Park ◽  
Kyoung Su Kim ◽  
Hee-Sool Rho ◽  
Myoung-Hwan Chi ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Magnaporthe Oryzae ◽  
Rice Blast ◽  
Rice Blast Fungus ◽  
Blast Fungus ◽  
Appressorium Development ◽  
Homeobox Transcription Factors

scholarly journals Factors involved in the duodenal expression of the human calbindin-D9k gene

1999 ◽  
Vol 341 (3) ◽  
pp. 491-500 ◽  
Author(s):  
Natalie F. BARLEY ◽  
S. Radhika PRATHALINGAM ◽  
Pang ZHI ◽  
Stephen LEGON ◽  
Alison HOWARD ◽  
...  
Keyword(s):  
Vitamin D ◽  
Transcription Factors ◽  
Regional Differences ◽  
Calcium Absorption ◽  
Receptor Protein ◽  
Distal Intestine ◽  
Calbindin D9k ◽  
Enhancer Binding Protein ◽  
Potential Binding ◽  
Mcf 7

Calbindin-D9k is expressed in the cytoplasm of intestinal cells, where it is critical for dietary calcium absorption. Two striking aspects of the expression of this gene are its vitamin-D dependency and regional differences in expression, with high levels only in duodenum. We report studies of the human calbindin-D9k promoter. Differences between the reported sequences of the human calbindin-D9k promoter were first clarified before undertaking a functional analysis of this sequence. Studies of the rat gene have indicated that several transcription factors, including the caudal-related homeobox factor (CDX-2), hepatic nuclear factor-4 and CCAAT-enhancer-binding protein α (C/EBPα), could interact with elements in the promoter. Although these elements are conserved in the human gene, we show here that their intestinal distribution makes them unlikely to be critical positive factors. The calbindin-D9k gene contains multiple potential binding sites for homeobox transcription factors; one of these, known as IPF-1 or PDX-1, co-localizes in the intestine with calbindin-D9k. We show in gel-shift assays that the sequence within a putative vitamin-D-response element in the human calbindin-D9k promoter can bind expressed IPF-1/PDX-1 protein, although we cannot confirm binding of the vitamin-D-receptor protein. CDX-2 binds to the region around the TATA box, as in the rat gene, and may act as a negative factor in the distal intestine. Transfection studies in Caco-2 and MCF-7 cells with heterologous reporter vectors containing up to 1303 bp of the gene showed that this functioned as a weak promoter and indicated the presence of suppressor sequences, but did not show vitamin-D responsiveness. This indicates that other elements are also needed for the control of human calbindin-D9k expression.

Engrailed homeobox transcription factors as potential markers and targets in cancer

FEBS Letters ◽  
2013 ◽  
Vol 587 (6) ◽  
pp. 549-554 ◽  
Author(s):  
Sophie E. McGrath ◽  
Agnieszka Michael ◽  
Hardev Pandha ◽  
Richard Morgan
Keyword(s):  
Transcription Factors ◽  
Homeobox Transcription Factors

Disturbed flow influences endothelial activation by inducing homeobox transcription factors

2014 ◽  
Vol 237 (2) ◽  
pp. e8
Author(s):  
Ismael Gauci ◽  
Marwa Mahmoud ◽  
Hayley Duckles ◽  
Shuang Feng ◽  
Sarah Hsiao ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Endothelial Activation ◽  
Disturbed Flow ◽  
Homeobox Transcription Factors

scholarly journals Common ancestry of heterodimerizing TALE homeobox transcription factors across Metazoa and Archaeplastida

2018 ◽  
Author(s):  
Sunjoo Joo ◽  
Ming Hsiu Wang ◽  
Gary Lui ◽  
Jenny Lee ◽  
Andrew Barnas ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Gene Regulatory Networks ◽  
Regulatory Networks ◽  
Bioinformatics Analysis ◽  
Land Plants ◽  
Protein Protein Interaction ◽  
Functional Conservation ◽  
Developmental Mechanisms ◽  
Gene Regulatory ◽  
Homeobox Transcription Factors

AbstractHomeobox transcription factors (TFs) in the TALE superclass are deeply embedded in the gene regulatory networks that orchestrate embryogenesis. Knotted-like homeobox (KNOX) TFs, homologous to animal MEIS, have been found to drive the haploid-to-diploid transition in both unicellular green algae and land plants via heterodimerization with other TALE superclass TFs, representing remarkable functional conservation of a developmental TF across lineages that diverged one billion years ago. To delineate the ancestry of TALE-TALE heterodimerization, we analyzed TALE endowment in the algal radiations of Archaeplastida, ancestral to land plants. Homeodomain phylogeny and bioinformatics analysis partitioned TALEs into two broad groups, KNOX and non-KNOX. Each group shares previously defined heterodimerization domains, plant KNOX-homology in the KNOX group and animal PBC-homology in the non-KNOX group, indicating their deep ancestry. Protein-protein interaction experiments showed that the TALEs in the two groups all participated in heterodimerization. These results indicate that the TF dyads consisting of KNOX/MEIS and PBC-containing TALEs must have evolved early in eukaryotic evolution, a likely function being to accurately execute the haploid-to-diploid transitions during sexual development.Author summaryComplex multicellularity requires elaborate developmental mechanisms, often based on the versatility of heterodimeric transcription factor (TF) interactions. Highly conserved TALE-superclass homeobox TF networks in major eukaryotic lineages suggest deep ancestry of developmental mechanisms. Our results support the hypothesis that in early eukaryotes, the TALE heterodimeric configuration provided transcription-on switches via dimerization-dependent subcellular localization, ensuring execution of the haploid-to-diploid transition only when the gamete fusion is correctly executed between appropriate partner gametes, a system that then diversified in the several lineages that engage in complex multicellular organization.

scholarly journals Homeobox Transcription Factors Are Required for Fungal Development and the Suppression of Host Defense Mechanisms in the Colletotrichum scovillei -Pepper Pathosystem

mBio ◽  
2021 ◽  
Author(s):  
Teng Fu ◽  
Joon-Hee Han ◽  
Jong-Hwan Shin ◽  
Hyeunjeong Song ◽  
Jaeho Ko ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Host Defense ◽  
Defense Mechanisms ◽  
Molecular Mechanisms ◽  
Yield Loss ◽  
Phytopathogenic Fungus ◽  
Fungal Development ◽  
Colletotrichum Scovillei ◽  
Homeobox Transcription Factors

The ascomycete phytopathogenic fungus, Colletotrichum scovillei , causes serious yield loss on peppers. However, little is known about molecular mechanisms involved in the development of anthracnose caused by this fungus.

scholarly journals Switch from Stress Response to Homeobox Transcription Factors in Adipose Tissue After Profound Fat Loss

PLoS ONE ◽  
2010 ◽  
Vol 5 (6) ◽  
pp. e11033 ◽  
Author(s):  
Simon N. Dankel ◽  
Dag J. Fadnes ◽  
Anne-Kristin Stavrum ◽  
Christine Stansberg ◽  
Rita Holdhus ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Transcription Factors ◽  
Stress Response ◽  
Fat Loss ◽  
Homeobox Transcription Factors
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