M1757 Impact of Luminal and Systemic Iron on the Development of Chronic Ileitis Targeting the Gut Microbial Composition and Stress Response in Intestinal Epithelial Cells

2010 ◽  
Vol 138 (5) ◽  
pp. S-413
Author(s):  
Tanja Werner ◽  
Stefan J. Wagner ◽  
Jung-Su Chang ◽  
Sigrid Kisling ◽  
Klaus Schuemann ◽  
...  
Keyword(s):  
Stress Response ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Microbial Composition

scholarly journals Identification microflora related to growth performance in pigs based on 16s rRNA sequence analyses

2020 ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Growth Performance ◽  
Intestinal Epithelial Cells ◽  
Average Daily Gain ◽  
Intestinal Flora ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors that affect the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n = 10), Landrace (n = 9) and Yorkshire (n = 21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. And we also analyzed their growth performance. We found that Duroc and Landrace had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and 100 kg daily age (P < 0.05). Moreover, through the analysis of the intestinal flora, we also identified that there were 18 species of intestinal flora with significant differences between Duroc and Landrace (P < 0.05). In order to eliminate the influence of genetic background, the intestinal differential flora of 21 Yorkshire pigs with different growth performance was analyzed. The results showed that there were significant correlations between Barnesiella, Dorea, Clostridium and Lactobacillus and pig growth performance. To explore the effect of intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus, which has the highest content in the intestine, was selected for isolation and purification, and co-cultured with intestinal epithelial cells. The qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in intestinal epithelial cells. The results provided useful reference for the further study on the relationship between intestinal flora and pig growth performance.

scholarly journals Delivery Mode Affects Intestinal Microbial Composition and the Development of Intestinal Epithelial Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Linzheng Lyu ◽  
Xiaohong Zhou ◽  
Meng Zhang ◽  
Li Liu ◽  
Haiyue Niu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Vaginal Delivery ◽  
Intestinal Epithelial Cells ◽  
Delivery Mode ◽  
Apparent Difference ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Delivery Modes ◽  
Rat Pups ◽  
Stool Samples

BackgroundThe infant’s intestine contains diverse microbiota, which play an important role in an infant’s health.ObjectiveThis study aimed to analyze the different intestinal microbiota and their function in two delivery modes [vaginal delivery and cesarean section (C-section)] and to investigate the proprieties of bacteria associated with vaginal delivery on the development of intestinal epithelial cells in rat pups.Materials and MethodsWe evaluated the intestinal microbial diversity of the stool samples of 51 infants of subjects who underwent vaginal delivery and C-section by sequencing the V4 regions of the 16S rRNA gene and predicted the function of the microbiotas. The infant stool microbiota in the vaginal delivery group was associated with the digestive system and cell growth and death, whereas that of the C-section group was associated with membrane transport. Then, we isolated the strains based on function prediction.ResultsA total of 95 strains were isolated in the vaginal delivery group. Bifidobacterium bifidum FL-228.1 (FL-228.1) was screened and selected owing to its good surface hydrophobicity, bacterial survivability in the simulated gastrointestinal condition and adhesion ability to the IEC-6 cell line as well as owing to the development of intestinal epithelial cells. Furthermore, in vivo experiments revealed that FL-228.1 exhibited favorable effects on the development of intestinal epithelial cells in rat pups.ConclusionThe results of this study indicate an apparent difference in the bacterial composition of the stool samples collected from infants of the two delivery modes. By analyzing and screening the bacteria in infant stool samples, we found that one strain, i.e., B bifidum FL-228.1, exhibited favorable effects on the development of intestinal epithelial cells.

Analysis of the role of the endoplasmic reticulum stress response-related molecule CHOP in intestinal epithelial cells

Impact ◽  
2021 ◽  
Vol 2021 (6) ◽  
pp. 18-20
Author(s):  
Yosuke Shimodaira
Keyword(s):  
Endoplasmic Reticulum ◽  
Stress Response ◽  
Epithelial Cells ◽  
Er Stress ◽  
Intestinal Epithelial Cells ◽  
Bloody Stool ◽  
Intestinal Epithelial ◽  
Stomach Pain ◽  
Intestinal Immune System

Inflammatory bowel diseases (IBDs) include ulcerative colitis and Crohn's disease. The symptoms experienced by people with IBD include diarrhea, bloody stool, extreme tiredness, weight loss and stomach pain. IBD is on the rise globally and it is unknown why. There is no existing cure for the condition and treatments are not always successful. In the quest to find a cure for IBD, researchers are investigating the mechanisms of the disease. Dr Yosuke Shimodaira, Department of Gastroenterology and Neurology, Akita University Graduate School of Medicine, Japan, is seeking to understand more about the mechanisms involved in the development of the disease. A key part of this research is autophagy and Shimodaira and the team are working to shed light on why endoplasmic reticulum (ER) stress in the intestinal epithelial cells induces autophagy and the role this might play in the development of IBD. A key learning for the team was that C/EBP-homologous protein (CHOP) is involved in the ER stress response. An important focus for the researchers is on the role played by CHOP in intestinal epithelial cells in vivo and its involvement in the pathophysiology of IBD. Shimodaira and the team plan to conduct experiments using mice and patients samples to analyse these fundamental mechanism in intestinal homeostasis. This will enable them to determine whether CHOP plays a role in vivo and how CHOP affect intestinal immune system.

Effects of mesalamine on the hsp72 stress response in rat IEC-18 intestinal epithelial cells

1997 ◽  
Vol 113 (5) ◽  
pp. 1474-1479 ◽  
Author(s):  
GC Burress ◽  
MW Musch ◽  
DA Jurivich ◽  
J Welk ◽  
EB Chang
Keyword(s):  
Stress Response ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial

scholarly journals Characterization and comparison of the bacterial community between complete intensive and extensive feeding patterns in pigs

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Resistance ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Flora ◽  
Feeding Pattern ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Drb Gene

AbstractTo investigate and compare the gut microbiota structures in complete intensive feeding pattern (CP) and extensive feeding pattern (EP) groups, a total of 20 pigs were divided into two groups and fed the same diet. The fecal microbial composition was profiled using 16S rRNA gene sequencing. Our results showed that seventeen predominant genera were present in each pig sample and constituted the phylogenetic core of the microbiota at the class level. The abundance of most of the core microbial flora were significantly higher in the CP group than in the EP group (P < 0.05), while the abundance of Gammaproteobacteria was significantly lower in the CP group than in the EP group (P < 0.05). The CP group had significantly greater community diversity, richness, and evenness than the EP group (P < 0.05). Functional prediction analysis indicated that intestinal microbial species potentially led to faster growth and an increased fat accumulation capacity in the CP group; however, disease resistance was weaker in the CP group than in the EP group. In conclusion, EP pigs have a wider range of activity and better animal welfare than CP pigs, which helps reduce the occurrence of diseases and neurological symptoms. To explore the effect of intestinal flora on disease resistance in pigs at the molecular level, Coprococcus, which is a key gut bacterium in the intestine, was selected for isolation and purification and cocultured with intestinal epithelial cells. qPCR was performed to determine the effect of Coprococcus on SLA-DRB gene expression in intestinal epithelial cells. The results showed that Coprococcus enhanced SLA-DRB gene expression in intestinal epithelial cells. The results provide useful reference data for further study on the relationship between intestinal flora and pig disease resistance.

scholarly journals Identification microflora related to growth performance in pigs based on 16s rRNA sequence analyses

2020 ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Growth Performance ◽  
Intestinal Epithelial Cells ◽  
Average Daily Gain ◽  
Intestinal Flora ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors that affect the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n=10), Landrace (n=9) and Yorkshire (n=21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. And we also analyzed their growth performance. We found that Duroc and Landrace had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and 100kg daily age ( P<0.05 ). Moreover, through the analysis of the intestinal flora, we also identified that there were 18 species of intestinal flora with significant differences between Duroc and Landrace ( P<0.05 ). In order to eliminate the influence of genetic background, the intestinal differential flora of 21 Yorkshire pigs with different growth performance was analyzed. The results showed that there were significant correlations between Barnesiella , Dorea , Clostridium and Lactobacillus and pig growth performance. To explore the effect of intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus , which has the highest content in the intestine, was selected for isolation and purification, and co-cultured with intestinal epithelial cells. The qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in intestinal epithelial cells. The results provided useful reference for the further study on the relationship between intestinal flora and pig growth performance.

scholarly journals Identification of microflora related to growth performance in pigs based on 16S rRNA sequence analyses

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Growth Performance ◽  
Intestinal Epithelial Cells ◽  
Average Daily Gain ◽  
Intestinal Flora ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors affecting the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n = 10), Landrace (n = 9) and Yorkshire (n = 21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. We also analyzed their growth performance. We found that Duroc and Landrace pigs had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and number of days taken to reach 100 kg (P < 0.05). Moreover, through analysis of the intestinal flora, we also identified 18 species of intestinal flora with significant differences between Duroc and Landrace pigs (P < 0.05). To eliminate the influence of genetic background, the differential intestinal flora of 21 Yorkshire pigs with differences in growth performance was analyzed. The results showed that there were significant correlations between Barnesiella, Dorea, Clostridium and Lactobacillus and pig growth performance. To explore the effect of the intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus, which is the most abundant in the intestine, was selected for isolation and purification and cocultured with intestinal epithelial cells. qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in these cells. The results provide a useful reference for further study of the relationship between the intestinal flora and pig growth performance.

scholarly journals Characterization and comparison of the bacterial community between complete intensive and extensive feeding patterns in pigs

2020 ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Resistance ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Flora ◽  
Feeding Pattern ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Drb Gene

Abstract To investigate and compare the gut microbiota structure in complete intensive feeding pattern (CP) and extensive feeding pattern (EP) groups, a total of 20 pigs were divided into two groups and fed the same diet. The fecal microbial composition was profiled using 16S rRNA gene sequencing. Our results showed that seventeen predominant genera were present in each pig sample and constituted a phylogenetic core microbiota at the class level. Most of the core microbial flora was significantly higher in the CP group than the EP group ( P < 0.05), while Gammaproteobacteria was significantly lower in the CP group than in the EP group ( P < 0.05). The CP group had significantly higher community diversity, richness, and evenness than the EP group ( P < 0.05). Functional prediction indicated that intestinal microbial species might lead to higher growth and an increased fat accumulation capacity in the CP group; however, disease resistance was weaker in the CP group than the EP group. In conclusion, EP pigs have a wider range of sports venues and better animal welfare than CP pigs, which helps reduce the occurrence of diseases and neurological symptoms. To explore the effect of intestinal flora on the disease resistance of pigs at the molecular level, Coprococcus, which has the key gut bacterium in the intestine, was selected for isolation and purification, and co-cultured with intestinal epithelial cells. The qPCR was used to determine the effect of Coprococcus on SLA-DRB gene expression in intestinal epithelial cells. The results showed that Coprococcus enhance SLA-DRB gene expression in intestinal epithelial cells. The results provided useful references for further study on the relationship between intestinal flora and pig disease resistance.

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