scholarly journals Delivery Mode Affects Intestinal Microbial Composition and the Development of Intestinal Epithelial Cells

2021 ◽  
Vol 12 ◽  
Author(s):  
Linzheng Lyu ◽  
Xiaohong Zhou ◽  
Meng Zhang ◽  
Li Liu ◽  
Haiyue Niu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Vaginal Delivery ◽  
Intestinal Epithelial Cells ◽  
Delivery Mode ◽  
Apparent Difference ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Delivery Modes ◽  
Rat Pups ◽  
Stool Samples

BackgroundThe infant’s intestine contains diverse microbiota, which play an important role in an infant’s health.ObjectiveThis study aimed to analyze the different intestinal microbiota and their function in two delivery modes [vaginal delivery and cesarean section (C-section)] and to investigate the proprieties of bacteria associated with vaginal delivery on the development of intestinal epithelial cells in rat pups.Materials and MethodsWe evaluated the intestinal microbial diversity of the stool samples of 51 infants of subjects who underwent vaginal delivery and C-section by sequencing the V4 regions of the 16S rRNA gene and predicted the function of the microbiotas. The infant stool microbiota in the vaginal delivery group was associated with the digestive system and cell growth and death, whereas that of the C-section group was associated with membrane transport. Then, we isolated the strains based on function prediction.ResultsA total of 95 strains were isolated in the vaginal delivery group. Bifidobacterium bifidum FL-228.1 (FL-228.1) was screened and selected owing to its good surface hydrophobicity, bacterial survivability in the simulated gastrointestinal condition and adhesion ability to the IEC-6 cell line as well as owing to the development of intestinal epithelial cells. Furthermore, in vivo experiments revealed that FL-228.1 exhibited favorable effects on the development of intestinal epithelial cells in rat pups.ConclusionThe results of this study indicate an apparent difference in the bacterial composition of the stool samples collected from infants of the two delivery modes. By analyzing and screening the bacteria in infant stool samples, we found that one strain, i.e., B bifidum FL-228.1, exhibited favorable effects on the development of intestinal epithelial cells.

scholarly journals Identification microflora related to growth performance in pigs based on 16s rRNA sequence analyses

2020 ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Growth Performance ◽  
Intestinal Epithelial Cells ◽  
Average Daily Gain ◽  
Intestinal Flora ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors that affect the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n = 10), Landrace (n = 9) and Yorkshire (n = 21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. And we also analyzed their growth performance. We found that Duroc and Landrace had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and 100 kg daily age (P < 0.05). Moreover, through the analysis of the intestinal flora, we also identified that there were 18 species of intestinal flora with significant differences between Duroc and Landrace (P < 0.05). In order to eliminate the influence of genetic background, the intestinal differential flora of 21 Yorkshire pigs with different growth performance was analyzed. The results showed that there were significant correlations between Barnesiella, Dorea, Clostridium and Lactobacillus and pig growth performance. To explore the effect of intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus, which has the highest content in the intestine, was selected for isolation and purification, and co-cultured with intestinal epithelial cells. The qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in intestinal epithelial cells. The results provided useful reference for the further study on the relationship between intestinal flora and pig growth performance.

scholarly journals Characterization and comparison of the bacterial community between complete intensive and extensive feeding patterns in pigs

AMB Express ◽  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Resistance ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Flora ◽  
Feeding Pattern ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Drb Gene

AbstractTo investigate and compare the gut microbiota structures in complete intensive feeding pattern (CP) and extensive feeding pattern (EP) groups, a total of 20 pigs were divided into two groups and fed the same diet. The fecal microbial composition was profiled using 16S rRNA gene sequencing. Our results showed that seventeen predominant genera were present in each pig sample and constituted the phylogenetic core of the microbiota at the class level. The abundance of most of the core microbial flora were significantly higher in the CP group than in the EP group (P < 0.05), while the abundance of Gammaproteobacteria was significantly lower in the CP group than in the EP group (P < 0.05). The CP group had significantly greater community diversity, richness, and evenness than the EP group (P < 0.05). Functional prediction analysis indicated that intestinal microbial species potentially led to faster growth and an increased fat accumulation capacity in the CP group; however, disease resistance was weaker in the CP group than in the EP group. In conclusion, EP pigs have a wider range of activity and better animal welfare than CP pigs, which helps reduce the occurrence of diseases and neurological symptoms. To explore the effect of intestinal flora on disease resistance in pigs at the molecular level, Coprococcus, which is a key gut bacterium in the intestine, was selected for isolation and purification and cocultured with intestinal epithelial cells. qPCR was performed to determine the effect of Coprococcus on SLA-DRB gene expression in intestinal epithelial cells. The results showed that Coprococcus enhanced SLA-DRB gene expression in intestinal epithelial cells. The results provide useful reference data for further study on the relationship between intestinal flora and pig disease resistance.

scholarly journals Identification microflora related to growth performance in pigs based on 16s rRNA sequence analyses

2020 ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Growth Performance ◽  
Intestinal Epithelial Cells ◽  
Average Daily Gain ◽  
Intestinal Flora ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors that affect the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n=10), Landrace (n=9) and Yorkshire (n=21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. And we also analyzed their growth performance. We found that Duroc and Landrace had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and 100kg daily age ( P<0.05 ). Moreover, through the analysis of the intestinal flora, we also identified that there were 18 species of intestinal flora with significant differences between Duroc and Landrace ( P<0.05 ). In order to eliminate the influence of genetic background, the intestinal differential flora of 21 Yorkshire pigs with different growth performance was analyzed. The results showed that there were significant correlations between Barnesiella , Dorea , Clostridium and Lactobacillus and pig growth performance. To explore the effect of intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus , which has the highest content in the intestine, was selected for isolation and purification, and co-cultured with intestinal epithelial cells. The qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in intestinal epithelial cells. The results provided useful reference for the further study on the relationship between intestinal flora and pig growth performance.

scholarly journals Identification of microflora related to growth performance in pigs based on 16S rRNA sequence analyses

AMB Express ◽  
2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Growth Performance ◽  
Intestinal Epithelial Cells ◽  
Average Daily Gain ◽  
Intestinal Flora ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors affecting the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n = 10), Landrace (n = 9) and Yorkshire (n = 21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. We also analyzed their growth performance. We found that Duroc and Landrace pigs had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and number of days taken to reach 100 kg (P < 0.05). Moreover, through analysis of the intestinal flora, we also identified 18 species of intestinal flora with significant differences between Duroc and Landrace pigs (P < 0.05). To eliminate the influence of genetic background, the differential intestinal flora of 21 Yorkshire pigs with differences in growth performance was analyzed. The results showed that there were significant correlations between Barnesiella, Dorea, Clostridium and Lactobacillus and pig growth performance. To explore the effect of the intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus, which is the most abundant in the intestine, was selected for isolation and purification and cocultured with intestinal epithelial cells. qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in these cells. The results provide a useful reference for further study of the relationship between the intestinal flora and pig growth performance.

scholarly journals Characterization and comparison of the bacterial community between complete intensive and extensive feeding patterns in pigs

2020 ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Disease Resistance ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Intestinal Flora ◽  
Feeding Pattern ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Drb Gene

Abstract To investigate and compare the gut microbiota structure in complete intensive feeding pattern (CP) and extensive feeding pattern (EP) groups, a total of 20 pigs were divided into two groups and fed the same diet. The fecal microbial composition was profiled using 16S rRNA gene sequencing. Our results showed that seventeen predominant genera were present in each pig sample and constituted a phylogenetic core microbiota at the class level. Most of the core microbial flora was significantly higher in the CP group than the EP group ( P < 0.05), while Gammaproteobacteria was significantly lower in the CP group than in the EP group ( P < 0.05). The CP group had significantly higher community diversity, richness, and evenness than the EP group ( P < 0.05). Functional prediction indicated that intestinal microbial species might lead to higher growth and an increased fat accumulation capacity in the CP group; however, disease resistance was weaker in the CP group than the EP group. In conclusion, EP pigs have a wider range of sports venues and better animal welfare than CP pigs, which helps reduce the occurrence of diseases and neurological symptoms. To explore the effect of intestinal flora on the disease resistance of pigs at the molecular level, Coprococcus, which has the key gut bacterium in the intestine, was selected for isolation and purification, and co-cultured with intestinal epithelial cells. The qPCR was used to determine the effect of Coprococcus on SLA-DRB gene expression in intestinal epithelial cells. The results showed that Coprococcus enhance SLA-DRB gene expression in intestinal epithelial cells. The results provided useful references for further study on the relationship between intestinal flora and pig disease resistance.

Sa1831 The Effects of Alterations in the Luminal Microbial Composition on Pyroptosis of Intestinal Epithelial Cells and Mucosal Inflammation

2016 ◽  
Vol 150 (4) ◽  
pp. S375
Author(s):  
Elisabeth M. Davis ◽  
Yuxiu Wang ◽  
Yihong Kaufmann ◽  
Keith Lai ◽  
Hannah Goyne ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Mucosal Inflammation ◽  
Intestinal Epithelial ◽  
Microbial Composition

scholarly journals Identification of microflora related to growth performance in pigs based on 16S rRNA sequence analyses

2020 ◽  
Author(s):  
Xin-Jian Li ◽  
Mingyu Wang ◽  
Yahui Xue ◽  
Dongdong Duan ◽  
Cong Li ◽  
...  
Keyword(s):  
Gene Expression ◽  
Epithelial Cells ◽  
Growth Performance ◽  
Intestinal Epithelial Cells ◽  
Average Daily Gain ◽  
Intestinal Flora ◽  
Rrna Gene ◽  
Intestinal Epithelial ◽  
Microbial Composition ◽  
Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors affecting the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n=10), Landrace (n=9) and Yorkshire (n=21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. We also analyzed their growth performance. We found that Duroc and Landrace pigs had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and number of days taken to reach 100 kg ( P<0.05 ). Moreover, through analysis of the intestinal flora, we also identified 18 species of intestinal flora with significant differences between Duroc and Landrace pigs ( P<0.05 ). To eliminate the influence of genetic background, the differential intestinal flora of 21 Yorkshire pigs with differences in growth performance was analyzed. The results showed that there were significant correlations between Barnesiella , Dorea , Clostridium and Lactobacillus and pig growth performance. To explore the effect of the intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus , which is the most abundant in the intestine, was selected for isolation and purification and cocultured with intestinal epithelial cells. qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in these cells. The results provide a useful reference for further study of the relationship between the intestinal flora and pig growth performance.

Three-dimensional organization and functional relationships of endocytotic compartments in pig intestinal epithelial cells

1992 ◽  
Vol 50 (1) ◽  
pp. 576-577
Author(s):  
Julian P. Heath ◽  
Buford L. Nichols ◽  
László G. Kömüves
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Intestinal Epithelial Cells ◽  
Three Dimensional ◽  
Intestinal Epithelial ◽  
Cell Surfaces ◽  
Basal Surface ◽  
Functional Relationships

The newborn pig intestine is adapted for the rapid and efficient absorption of nutrients from colostrum. In enterocytes, colostral proteins are taken up into an apical endocytotic complex of channels that transports them to target organelles or to the basal surface for release into the circulation. The apical endocytotic complex of tubules and vesicles clearly is a major intersection in the routes taken by vesicles trafficking to and from the Golgi, lysosomes, and the apical and basolateral cell surfaces.Jejunal tissues were taken from piglets suckled for up to 6 hours and prepared for electron microscopy and immunocytochemistry as previously described.

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