Phytocompounds of Bistorta macrophylla (D. Don) Sojak. as bioavailability enhancers of fluconazole and amphotericin B to better manage Candida species infections

Bistorta macrophylla (D. Don) Sojak. is a medicinal plant of high altitude and so far, not been scientifically explored? Since prehistoric times, B. macrophylla has been used to cure stomach pain, pyretic fever, flu, lungs infections, diarrhea, vomiting. The present research was aimed to examine the phytochemicals, antifungal, and synergistic potential of methanolic extracts of B. macrophylla. Methanolic extract of B. macrophylla was found to have high phenolic (191.18 ± 29.18 mg g−1 GAE) and flavonoid (26.71 ± 3.21 mg g−1 RE) content. Methanolic extract also demonstrate strong antifungal action with diameter of zone of inhibition of 17.5±0.5 mm (fungicidal) against both the strains of C. albicans (MTCC277 and ATCC90028). The minimal inhibitory concentration (MIC) of methanolic extract was found to be 62.5 µg ml−1 against C. albicans (MTCC277 and ATCC90028). In addition, the combination of methanolic extract of B. macrophylla with antifungal antibiotics (fluconazole and amphotericin B) showed synergistic interaction with MIC reduction from 4-128 folds against both candida strains. GC-MS analysis of methanolic extract revealed the presence of 15 major phytocompounds with area more than 1%. Molecular docking showed that sucrose and 9,9-Dimethoxybicyclo [ 3.3.1] nona-2,4-dione has highest binding energy of -6.3 and -5.1 KJ/mol against Cytochrome P450 14 alpha-sterol Demethylase (PDB ID: 1EA1) protein respectively. Combination of methanolic extract of B. macrophylla with antifungal antibiotics (fluconazole, amphotericin B) can be used to treat drug-resistant candida.

Lasia spinosa Chemical Composition and Therapeutic Potential: A Literature-Based Review

Lasia spinosa (L.) is used ethnobotanically for the treatment of various diseases, including rheumatoid arthritis, inflammation of the lungs, bleeding cough, hemorrhoids, intestinal diseases, stomach pain, and uterine cancer. This review is aimed at summarizing phytochemistry and pharmacological data with their molecular mechanisms of action. A search was performed in databases such as PubMed, Science Direct, and Google Scholar using the keywords: “Lasia spinosa,” then combined with “ethnopharmacological use,” “phytochemistry,” and “pharmacological activity.” This updated review included studies with in vitro, ex vivo, and in vivo experiments with compounds of known concentration and highlighted pharmacological mechanisms. The research results showed that L. spinosa contains many important nutritional and phytochemical components such as alkanes, aldehydes, alkaloids, carotenoids, flavonoids, fatty acids, ketones, lignans, phenolics, terpenoids, steroids, and volatile oil with excellent bioactivity. The importance of this review lies in the fact that scientific pharmacological evidence supports the fact that the plant has antioxidant, anti-inflammatory, antimicrobial, cytotoxic, antidiarrheal, antihelminthic, antidiabetic, antihyperlipidemic, and antinociceptive effects, while protecting the gastrointestinal system and reproductive. Regarding future toxicological and safety data, more research is needed, including studies on human subjects. In light of these data, L. spinosa can be considered a medicinal plant with effective bioactives for the adjuvant treatment of various diseases in humans.

LINGUISTIC AND INFORMATIVE FEATURES OF STUDYING THE THEME “PROFESSIONAL COMMUNICATION A PHYSICIAN AND A PATIENT WITH SYMPTOMS DISEASES OF DIGESTIVE SYSTEM’S ORGANS” OF DISCIPLINE “PROFESSIONAL MEDICAL COMMUNICATION OF A DOCTOR WITH A PATIENT IN UKRAINIAN

The article presents educational, informative and methodical materials necessary for studying the theme “Professional communication a physician and a patient with symptoms diseases of the digestive system’s organs” in classes on the discipline “Professional medical communication of a doctor with a patient in Ukrainian language”. The complex of tasks is aimed at the development of students’ communicative skills and abilities: to study the vocabulary to denote the organs of the digestive system, gastrointestinal diseases; be able to build monologue and dialogic expressions that describe the causes and symptoms of the digestive system’s diseases, using learned lexical units and phrases; to develop skills of collecting the anamnesis of gastroenterological diseases; memorize phrases and sentences for first aid in food poisoning, in emergencies during stomach pain; to create modern informative and expert systems for developing lesson’s materials. The proposed system of tasks will help to master the skills and abilities to communicate orally and in writing in accordance with the goals and social norms of speech behavior in typical spheres and situations. Taking into account different methods and forms of work, the tasks with which it was possible to ensure the active participation of each student in the class, to stimulate interest and desire to study medical terminology in accordance with the topic of the class are singled out. It was suggested a variety of test and creative tasks to check the knowledge of the student’s studied topic. The materials described in the article are intended for foreign students of medical specialties who speak the language at a sufficient level. Tasks selected on the basis of four main types of speech activity (listening, speaking, reading, writing) will help foreign students not only to expand their vocabulary, but also to achieve social interaction in a foreign language professional sphere.

Using Herbal Medicine for Dental Pain in Saudi Arabia: Prevalence and Knowledge Assessment

Background: The dental community has started to use natural plant properties to relieve dental pain. Besides their significant serious side effect, Herb’s ingredients should be chosen wisely. However, many studies are conducted to find out the herb’s information due to the lack of randomized controlled clinical trials. The aim of this study was to assess the prevalence and the knowledge of the effect of using herbal medicine for dental pain among Saudi population. Methodology: An observational cross-sectional study was conducted in Saudi Arabia between September 2020 and October 2021. based on a structured questionnaire among Saudi populations. Structured self-administrated questionnaires in English and Arabic languages were used as a study tool. Authors collected the information using social media channels through google forms. SPSS 26 was used for data entry and data analysis. Results: The study included 4213 participants. 25% of study sample were males and 75% were females. 51.6% aged between 20- 30 years old. 61.8% of all participants use herbs to relieve toothache (82% of them use clove or clove oil and 36% use thyme). Think herbs are safe and free from side effects in treating toothache. 8% noticed side effects or damage while using herbs to relieve dental pain (27% of them swelling, 21% sharp stomach pain, 21% hypersensitive and 17% bleeding). Conclusion: The Saudi population show relatively good knowledge level and practice towards herbs use in dentistry. The use of herbs to relieve toothache was significantly associated with female gender, age, residence area in western region and average monthly income.

Properties of a Transport Instrument for Measuring Psychological Impacts of Delay on Commuters, Mokken Scale Analysis

This study assessed the validity of instrument including various negative psychological and physical behaviors of commuters due to the public transport delay. Instruments have been mostly evaluated by parametric method of item response theory (IRT). However, the IRT has been characterized by some restrictive assumptions about the data, focusing on detailed model fit evaluation. The Mokken scale analysis (MSA), as a scaling procedure is a non-parametric method, which does not require adherence to any distribution. The results of the study show that in most regards, our instrument meets the minimum requirements highlighted by the MSA. However, the instrument did not adhere to the minimum requirements of the “scalability” for two variables including “stomach pain” and “increased heart rate”. So, modifications were proposed to address the violations. Although MSA technique has been used frequently in other fields, this is one of the earliest studies to implement the technique in the context of transport psychology.

Phytochemistry, Pharmacology, and Nutraceutical Profile of Carissa Species: An Updated Review

Carissa, a genus of the Apocynaceae family, consists of evergreen species, such as shrubs as well as small trees that are native to Asia, Africa, and Oceania’s subtropical and tropical regions. Most of the Carissa species are traditionally used to treat various diseases, such as chest pain, headaches, gonorrhoea, rheumatism, syphilis, oedema, rabies, stomach pain, hepatitis, cardiac diseases, and asthma. The pharmacological studies on Carissa species revealed its antioxidant, antimicrobial, anticancer, cardioprotective, antipyretic, analgesic, wound healing, anticonvulsant, antiarthritic, adaptogenic, anti-inflammatory, and antidiabetic activities, thus validating its use in indigenous medicine systems. The review article summarised the comprehensive literature available, including morphology, indigenous uses, bioactive composition, nutraceutical, and pharmacological activities of Carissa species. A total of 155 research papers were cited in this review article. The Carissa fruits are rich in dietary fibre, lipids, proteins, carbohydrates, vitamin C, and macro- and micro-elements. A total of 121 compounds (35 polyphenols (flavonoids and phenolic acids), 30 lignans, 41 terpenoids, 7 steroids, 2 coumarins, and 6 cardiac glycosides) have been extracted from C. spinarum, C. carandas, and C. macrocarpa. Among all chemical constituents, lupeol, carissol, naringin, carisssone, scopoletin, carissaeduloside A, D, J, carandinol, sarhamnoloside, carissanol, olivil, carinol, 3β-hydroxyolean-11-en-28,13β-oilde, ursolic acid, and carissone are the key bioactive constituents responsible for pharmacological activities of genus Carissa. The gathered ethnopharmacological information in the review will help to understand the therapeutic relevance of Carissa as well as paving a way for further exploration in the discovery of novel plant-based drugs.

Histopathological Distribution of the Gastrointestinal Tract Lesions

Introduction: Gastrointestinal (GI) diseases are common and can affect any portion of the gastrointestinal tract from the mouth to the anus. Diseases can just shows clinical conditions like stomach pain, constipation, diarrhea which can be self- limiting. But sometimes disease may be life-threatening like malignancy. Biopsy is necessary for confirmatory diagnosis and further treatment of the patient. So histopathologic examination is a must for all surgical procedures for confirmation and categorization of GI disorders. Objectives: This study was done to find out the various patterns of lesions of the gastrointestinal tract with its commonest age group and sex involvement Methodology: This was a prospective study of all the surgically resected GI tissue received in the Department of Pathology Histopathology unit in Birat Medical College and Teaching Hospital (BMCTH) from 1st February 2021 to 30 thApril 2021.The hematoxylin and eosin stain slides of the GI tissue received were studied and the lesions were diagnosed on their histomorphology. According to organ, age and sex, the lesions were categorized. The data were entered in Microsoft excel and the percentage value was calculated. Results: Out of the total 344 cases 146(42.44%) were male and 198(57.56%) were female patients. The most common age range for GI lesions was 41 to 60 years comprising of 126(36.62%) of total cases. Maximum numbers of cases 160 (46.50%) were of cholecystectomies followed by appendectomies 95(27.60%). Inflammatory and benign lesions comprised 332(96.51%), 9(2.61%) were malignant tumor and 3(0.88%) were premalignant lesions. The most common inflammatory lesions and malignant tumors were chronic cholecystitis and gastric adenocarcinoma respectively Conclusions: The study identifies that gastrointestinal lesions comprise of the most common biopsies received in the histopathology department. Early diagnosis of premalignant and malignant lesions can improve the overall survival rate of patients.

A Review of Severe Acute Respiratory Syndrome Coronavirus 2 and Pathological Disorders in Patients

Firstly, a new coronavirus emerged in Wuhan, China, sparking a pandemic of acute respiratory syndrome in humans. Corona virus disease-19 (COVID-19) was declared as a pandemic after the infection spread globally and disease caused by the new type of coronavirus, Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) was named as COVID-19. The virus originated from bats and has been passed to humans. Initially, SARS-CoV-19 infects respiratory system an acute serious viral infection mainly developed with over a million people worldwide were transmitted rapidly in a few months. SARS-CoV-19, progress a moderate to serious severe respiratory symptoms such as headache, cough, and breathlessness. Also, digestive system symptoms include nausea, vomiting, and stomach pain. Although the precise of the mechanism of inflammation unclear but this virus firstly use the angiotensin converting enzyme 2 (ACE2) receptor of the alveolar cells of lung tissue causing inflammation and cell death. Then virus spreads and affects other important organ and tissues with complex pathophysiological alterations, the coronary disease, pulmonary disease, chronic kidney disease and chronic liver disease are the most prevalent comorbidities among patients. In COVID-19 infection therapy lots agents recommended. Unfortunately, there is no clear effective drug for treatment. This review presented potential pathogenic pathway of the SARS-CoV-19 infection and symptoms in the patients and given the information about the negative effects on different systems in the respiratory, cardiac system, nervous system, gastrointestinal system, and urinary tract.

Ethnoveterinary Uses of Certain Yemeni Plants: A Review of the Scientific Evidence

Livestock is an important and integral component of agriculture production in Yemen and contributes 28% of the total agricultural production income. Research in the field of Yemeni ethnoveterinary medicine is limited to a few studies. Therefore, our work aims to substantiate scientifically the ethnoveterinary use of some documented plant species based on a literature review of their bioactivities and toxicological properties. Searching the scientific literature has revealed various pharmacological activities that may support the claimed healing activities of 11 out of 14 plant species for some of their ethnoveterinary utilization. This comprises the use of Aloe spp. latex for constipation, worms, boils, and wounds; Boswellia sacra underbark for wounds and its oleo-gum resin for mastitis; Soqotraen Boswellia species as an insect repellent; Cissus rotundifolia for stomach pain; Cyphostemma digitatum as an appetite stimulant; Psiadia punctulate for bone fracture; Pulicaria undulata as an insect repellent; combinations of Aristolochia bracteolate with Sorghum bicolor grains for bloating; Rumex nervosus and salt for eye pimples; and Trigonella foenum-graecum seeds with Hordeum vulgare grains for constipation. Some plants were found to demonstrate various toxic effects in in vivo and in vitro experimental studies. The local administration of Calotropis procera latex was also reported to induce an intense inflammatory response. It can be concluded that our work has provided valuable scientific information on the biological and toxic activities of some Yemeni ethnoveterinary remedies that could be utilized for the benefit of farmers to ration the use of these remedies and avoiding their toxicity.

Ethnobotanical study of the use of medicinal plants in the indigenous Pijao community in Natagaima, Colombia

Semi-structured interviews (80) were applied in order to document the medicinal plants used by the Pijao indigenous community in Natagaima, Colombia. As a result, a total of 110 species distributed in 54 families were registered, Asteraceae and Fabaceae (9% each) being the most important families. Regarding the parts of the plant, the most used structure was leaves (46.7%), the main form of preparation was infusion (32%), and the most used way of administration was oral (77.8%). The value of use of the species by informants (VUis), the relative importance of medicinal species (IRE) and the index of cultural value of medicinal species (IVUs) were determined. The most important species according to their value of use were: Tamarindus indica L., Psidium guajava L., Mentha x piperita L., Moringa oleifera Lam. The most reported ailments were gastrointestinal problems such as stomach pain and diarrhea, general aches, headache, fever, swollen liver, and respiratory problems.