554 A High Protein Diet Reduces Body Fat Mass and Alters the Gut Microbiome, With Expansion of Akkermansia, in a Rat Model of Diet-Induced Obesity

Isoliquiritigenin (ILG) is a flavonoid constituent of Glycyrrhizae plants. The current study investigated the effects of ILG on diet-induced obesity and metabolic diseases. C57BL/6J mice were fed a normal diet (AIN-76 purified diet), high-fat diet (40 kcal% fat), and high-fat diet +0.02% (w/w) ILG for 16 weeks. Supplementation of ILG resulted in decreased body fat mass and plasma cholesterol level. ILG ameliorated hepatic steatosis by suppressing the expression of hepatic lipogenesis genes and hepatic triglyceride and fatty acid contents, while enhancing β-oxidation in the liver. ILG improved insulin resistance by lowering plasma glucose and insulin levels. This was also demonstrated by the intraperitoneal glucose tolerance test (IPGTT). Additionally, ILG upregulated the expression of insulin signaling-related genes in the liver and muscle. Interestingly, ILG elevated energy expenditure by increasing the expression of thermogenesis genes, which is linked to stimulated mitochondrial biogenesis and uncoupled cellular respiration in brown adipose tissue. ILG also suppressed proinflammatory cytokine levels in the plasma. These results suggest that ILG supplemented at 0.02% in the diet can ameliorate body fat mass, plasma cholesterol, non-alcoholic fatty liver disease, and insulin resistance; these effects were partly mediated by increasing energy expenditure in high-fat fed mice.

Download Full-text

The Effect of Super Set Weight Training Model and a High-Protein Diet on Body Fat Level Changes in Overweight and Obese Adult Men

Proceedings of the 4th International Conference on Sport Science, Health, and Physical Education (ICSSHPE 2019) ◽

10.2991/ahsr.k.200214.051 ◽

2020 ◽

Author(s):

Reshandi Nugraha ◽

Adang Suherman ◽

Hamidie Ronald Daniel Ray ◽

Amung Ma’mun

Keyword(s):

Body Fat ◽

Weight Training ◽

High Protein Diet ◽

Training Model ◽

High Protein ◽

Protein Diet ◽

Obese Adult ◽

Adult Men ◽

Fat Level

Download Full-text

Comparison of body composition changes between low calorie high protein diet to standard protein in obese individuals with weight cycling – a randomised trial

F1000Research ◽

10.12688/f1000research.13300.1 ◽

2018 ◽

Vol 7 ◽

pp. 445 ◽

Cited By ~ 2

Author(s):

Joan Jutamulia ◽

Adventia Natali Paranoan ◽

Septian Ika Prasetya ◽

Ninik Mudjihartini ◽

Fiastuti Witjaksono

Keyword(s):

Body Composition ◽

Fat Mass ◽

High Protein Diet ◽

High Protein ◽

Protein Diet ◽

Weight Cycling ◽

Low Calorie ◽

Protein Group ◽

History Of ◽

Standard Protein

Background: The world prevalence of obesity in adult population in 2014 was nearly 13% while in Indonesia, it has reached 32.9% in the same year. Obesity is an established risk factors for cardiovascular diseases. A large proportion of people who had succeeded to reduce body weight failed to maintain it (weight cycling). Studies were inconclusive about the best composition in the diet for such people. The purpose of this research was to evaluate the body composition changes resulted from low calorie high protein and standard protein diet programme in obese people with history of weight cycling. Methods: This is an open-randomized clinical trial of weight loss program as a part of a larger study researching the effect of low calorie high protein diet to body composition, oxidative stress, inflammation marker and metabolic syndrome in obese with weight cycling. A total of 61 adult obese men and women with history of weight cycling were recruited. Subjects were assigned to low calorie diet and were randomly distributed into two intervention groups, namely high protein group (22-30 % of total calories intake) and standard protein group (12-20%). Anthropometry and body composition data were taken at baseline and at the end of the study. Subjects were followed up to 8 weeks with daily reminder and weekly counselling. Results: Fourty five participants completed the study. There were significant reductions in in body weight and body mass index (BMI), fat mass, fat mass percentage, muscle mass, and gain in muscle mass percentage occurred in both groups (Standard protein: P<0.001; High protein: P<0.001 for all variables except for fat mass P= 0,001) with no difference between group (P>0.05). Conclusion: Calories deficit with either high protein or standard protein for 8 weeks brought about significant reduction in body composition

Download Full-text

Mo1323 High Protein Diet Improves Body Mass Index and Visceral Adiposity, and Induces Satiety in a Human and Rat Model of Obesity

Gastroenterology ◽

10.1016/s0016-5085(15)32267-8 ◽

2015 ◽

Vol 148 (4) ◽

pp. S-671-S-672

Author(s):

Deepinder Goyal ◽

Lixin Wang ◽

Gigi Kwok ◽

Jihane Benhammou ◽

Gordon V. Ohning ◽

...

Keyword(s):

Body Mass Index ◽

Body Mass ◽

Rat Model ◽

High Protein Diet ◽

Visceral Adiposity ◽

High Protein ◽

Protein Diet

Download Full-text

High Protein Diet Selectively Reduces Fat Mass and Improves Glucose Tolerance in Diet-Induced Obese (DIO) Rats

Gastroenterology ◽

10.1016/s0016-5085(11)61352-8 ◽

2011 ◽

Vol 140 (5) ◽

pp. S-333

Author(s):

Andreas Stengel ◽

Miriam Goebel ◽

Lixin Wang ◽

Eugenia Hu ◽

Joseph R. Pisegna ◽

...

Keyword(s):

Glucose Tolerance ◽

Fat Mass ◽

High Protein Diet ◽

High Protein ◽

Protein Diet

Download Full-text

Effects of a High-Protein Diet on Insulin Resistance and Body Fat in Catch-Up Growth Rats Born Small for Gestational Age

Hormone Research in Paediatrics ◽

10.1159/000343149 ◽

2012 ◽

Vol 78 (3) ◽

pp. 180-187 ◽

Cited By ~ 1

Author(s):

Hong-Zhu Deng ◽

Hong Deng ◽

Yan-Hong Li ◽

Zhe Su ◽

Hua-Mei Ma ◽

...

Keyword(s):

Insulin Resistance ◽

Body Fat ◽

Gestational Age ◽

Small For Gestational Age ◽

High Protein Diet ◽

High Protein ◽

Protein Diet ◽

Catch Up

Download Full-text

A high-protein diet for reducing body fat: mechanisms and possible caveats

Nutrition & Metabolism ◽

10.1186/1743-7075-11-53 ◽

2014 ◽

Vol 11 (1) ◽

pp. 53 ◽

Cited By ~ 90

Author(s):

Dominik H Pesta ◽

Varman T Samuel

Keyword(s):

Body Fat ◽

High Protein Diet ◽

High Protein ◽

Protein Diet

Download Full-text

Effects of Combined High-Protein Diet and Exercise Intervention on Cardiometabolic Health in Middle-Aged Obese Adults: A Randomized Controlled Trial

Frontiers in Cardiovascular Medicine ◽

10.3389/fcvm.2021.705282 ◽

2021 ◽

Vol 8 ◽

Author(s):

Chiao-Nan Chen ◽

Kuo-Jen Hsu ◽

Kuei-Yu Chien ◽

Jeu-Jung Chen

Keyword(s):

Insulin Sensitivity ◽

Fat Mass ◽

Exercise Intervention ◽

High Protein Diet ◽

High Protein ◽

Protein Diet ◽

Cardiometabolic Health ◽

Peak Time ◽

Middle Aged ◽

Middle Aged Adults

Background: Obesity is the main risk factor of cardiovascular diseases (CVD) and metabolic diseases. The middle-aged population is the age group with the highest prevalence of obesity. Thus, improving cardiometabolic health is important to prevent CVD and metabolic diseases in middle-aged obese adults. The aim of this study was to examine the effects of exercise alone or in combination with a high-protein diet on markers of cardiometabolic health in middle-aged adults with obesity.Methods: Sixty-nine middle-aged adults with obesity were assigned randomly to the control group (C; n = 23), exercise group (E; n = 23), or exercise combined with high-protein diet group (EP; n = 23). Individuals in the E and EP groups received supervised exercise training and individuals in the EP group received high-protein diet intervention. Body composition (assessed by dual-energy X-ray absorptiometry), oral glucose tolerance test (OGTT), lipid profiles, and inflammatory markers were determined before and after 12 weeks of intervention. Insulin sensitivity index (ISI0,120) was calculated from values of fasting and 2-h insulin and glucose concentration of OGTT. Insulin-peak-time during the OGTT was recorded to reflect β-cell function. Analysis of covariance with baseline values as covariates was used to examine the effects of the intervention. The significant level was set at 0.05.Results: After 12 weeks of intervention, the E group had a greater percentage of individuals with early insulin-peak-time during the OGTT than that in the C and EP groups (p = 0.031). EP group had lower total cholesterol and triglycerides than that in the C group (p = 0.046 and 0.014, respectively). Within-group comparisons showed that the 2-h glucose of OGTT and C-reactive protein decreased in the EP group (p = 0.013 and 0.008, respectively) but not in the E and C groups; insulin sensitivity improved in the EP group (p = 0.016) and had a trend to improve in the E group (p = 0.052); and abdominal fat mass and total body fat mass decreased in both intervention groups (p < 0.05).Conclusion: Combined high-protein diet and exercise intervention significantly decreased fat mass and improved lipid profiles, insulin sensitivity, glucose tolerance, and inflammation in middle-aged adults with obesity.Clinical Trial Registration: Thai Clinical Trials Registry, TCTR20180913003, 13-09-2018.

Download Full-text