Su1238 LACTOBACILLUS RHAMNOSUS GG COLONIZATION IN EARLY LIFE AMELIORATES INFLAMMAGING OF OFFSPRING

Abstract Visceral hypersensitivity is a hallmark of many functional and stress-related gastrointestinal disorders, and there is growing evidence that the gut microbiota may play a role in its pathophysiology. It has previously been shown that early life stress-induced visceral sensitivity is reduced by various probiotic strains of bacteria (including Lactobacillus rhamnosus GG (LGG)) alone or in combination with prebiotic fibres in rat models. However, the exact mechanisms underpinning such effects remain unresolved. Here, we investigated if soluble mediators derived from LGG can mimic the bacteria’s effects on visceral hypersensitivity and the microbiota–gut–brain axis. Rats were exposed to maternal separation (MS) from postnatal days 2–12. From weaning onwards both non-separated (NS) and MS offspring were provided drinking water with or without supplementation of standardized preparations of the LGG soluble mediators (LSM). Our results show that MS led to increased visceral sensitivity and exaggerated corticosterone plasma levels following restraint stress in adulthood, and both of these effects were ameliorated through LSM supplementation. Differential regulation of various genes in the spinal cord of MS versus NS rats was observed, 41 of which were reversed by LSM supplementation. At the microbiota composition level MS led to changes in beta diversity and abundance of specific bacteria including parabacteroides, which were ameliorated by LSM. These findings support probiotic soluble mediators as potential interventions in the reduction of symptoms of visceral hypersensitivity.

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Supplementation of p40, a Lactobacillus rhamnosus GG-derived protein, in early life promotes epidermal growth factor receptor-dependent intestinal development and long-term health outcomes

Mucosal Immunology ◽

10.1038/s41385-018-0034-3 ◽

2018 ◽

Vol 11 (5) ◽

pp. 1316-1328 ◽

Cited By ~ 15

Author(s):

Xi Shen ◽

Liping Liu ◽

Richard M. Peek ◽

Sari A. Acra ◽

Daniel J. Moore ◽

...

Keyword(s):

Epidermal Growth Factor Receptor ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Early Life ◽

Lactobacillus Rhamnosus ◽

Growth Factor Receptor ◽

Intestinal Development ◽

Lactobacillus Rhamnosus Gg ◽

Epidermal Growth

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Sa1131 – Lactobacillus Rhamnosus Gg Colonization in Early Life Protects Intestinal Barrier of Offspring and Relieves Anxiety-Like Behavior in Adulthood

Gastroenterology ◽

10.1016/s0016-5085(19)37514-6 ◽

2019 ◽

Vol 156 (6) ◽

pp. S-278-S-279

Author(s):

Bingqian Zhou ◽

Ge Jin ◽

Xiang Liu ◽

Runxiang Xie ◽

Lan Zhang ◽

...

Keyword(s):

Early Life ◽

Lactobacillus Rhamnosus ◽

Intestinal Barrier ◽

Lactobacillus Rhamnosus Gg

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Lactobacillus rhamnosus GG Colonization in Early Life Ameliorates Inflammaging of Offspring by Activating SIRT1/AMPK/PGC-1α Pathway

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/3328505 ◽

2021 ◽

Vol 2021 ◽

pp. 1-27

Author(s):

Tianyu Liu ◽

Xueli Song ◽

Yaping An ◽

Xuemei Wu ◽

Wanru Zhang ◽

...

Keyword(s):

Early Life ◽

Lactobacillus Rhamnosus ◽

Intestinal Barrier ◽

Low Grade ◽

Peroxisome Proliferator ◽

Sequencing Analysis ◽

Lactobacillus Rhamnosus Gg ◽

Peroxisome Proliferator Activated Receptor ◽

Age Related ◽

Low Grade Inflammation

Inflammaging refers to chronic, low-grade inflammation during aging, which contributes to the pathogenesis of age-related diseases. Studies have shown that probiotic intervention in the aging stage could delay aging-related disorders. However, whether the application of probiotics in early life could have antiaging effects on offspring was unknown. Here, we investigated the effects of Lactobacillus rhamnosus GG (LGG) colonization in early life on inflammaging of offspring. Pregnant mice with the same conception time were given LGG live bacteria (LC group) or LGG fixed bacteria (NC group) from the 18th day after pregnancy until natural birth. The progeny mice were treated with 107 cfu of live or fixed LGG for 0-5 days after birth, respectively. LGG colonization could be detected in the feces of 3-week offspring. The 16S rRNA sequencing analysis of 3-week-old offspring showed that colonization of LGG in early life could alter the composition and diversity of gut microbiota. Interestingly, the beneficial effects of LGG colonization in early life on the microbiota lasted to 8 months old. The abundance of longevity-related bacteria (Lactobacillus, Bifidobacterium, and Akkermansia muciniphila) increased significantly in the LGG colonization group. In addition, LGG colonization increased the abundance of short-chain fatty acid- (SCFA-) producing bacteria and the production of cecal SCFAs. LGG colonization in early life protected the intestinal barrier, enhanced antioxidant defense, attenuated epithelial cell DNA damage, and inhibited intestinal low-grade inflammation in 8-month-old progeny mice. Mechanically, LGG could upregulate Sirtuin1 (SIRT1)/Adenosine 5 ′ -monophosphate-activated protein kinase (AMPK)/Peroxisome proliferator-activated receptor γ coactivator 1-α (PGC-1α) pathway and repress activation of nuclear factor-kappa B (NF-κB), while the protective effect of LGG was blunted after SIRT1 gene silencing. Together, LGG colonization in early life could ameliorate inflammaging of offspring, which would provide a new strategy for the prevention of age-related diseases.

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