Differential expression and cellular localization of somatolactin-1 and -2 during early development in the gilthead sea bream

Large-scale gene expression studies were performed for one of the main European aquaculture species, the gilthead sea bream Sparus auratus L. For this purpose, a cDNA microarray containing 10,176 clones from a cDNA library of mixed embryonic and larval stages was constructed. In addition to its importance for aquaculture, the taxonomic position and the relatively small genome size of sea bream makes it a prospective model for evolutionary biology and comparative genomics. However, so far, no large-scale analysis of gene expression exists for this species. In the present study, gene expression was analyzed in gilthead sea bream during early development, a significant period in the determination of quantitative traits and therefore of considerable interest for aquaculture. Synexpression groups expressed primarily early and late in development were determined and were composed of both known and novel genes. Furthermore, it was possible to identify stress response genes induced by cortisol injections using the cDNA microarray generated. The creation of gene expression profiles for sea bream by microarray hybridization will accelerate identification of candidate genes involved in multifactorial traits and certain regulatory pathways and will also contribute to a better understanding of the genetic background of fish physiology, which may help to improve aquaculture practices.

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Differential expression of PTHrP and its receptor in pituitary gland and gills in estradiol-treated gilthead sea bream (Sparus auratus, L.)

General and Comparative Endocrinology ◽

10.1016/j.ygcen.2011.05.017 ◽

2011 ◽

Vol 173 (1) ◽

pp. 199-204 ◽

Cited By ~ 2

Author(s):

Gideon S. Bevelander ◽

Pedro M. Guerreiro ◽

Tom Spanings ◽

Gert Flik

Keyword(s):

Pituitary Gland ◽

Differential Expression ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Sparus Auratus

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Stress effects of amyloodiniosis in gilthead sea bream Sparus aurata

Diseases of Aquatic Organisms ◽

10.3354/dao03199 ◽

2018 ◽

Vol 127 (3) ◽

pp. 201-211 ◽

Cited By ~ 3

Author(s):

M Moreira ◽

M Herrera ◽

P Pousão-Ferreira ◽

JI Navas Triano ◽

F Soares

Keyword(s):

Sparus Aurata ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Stress Effects

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Ingestion of plastic and non-plastic microfibers by farmed gilthead sea bream (Sparus aurata) and common carp (Cyprinus carpio) at different life stages

The Science of The Total Environment ◽

10.1016/j.scitotenv.2021.146851 ◽

2021 ◽

Vol 782 ◽

pp. 146851

Author(s):

S. Savoca ◽

K. Matanović ◽

G. D'Angelo ◽

V. Vetri ◽

S. Anselmo ◽

...

Keyword(s):

Common Carp ◽

Cyprinus Carpio ◽

Sparus Aurata ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Life Stages ◽

Common Carp Cyprinus Carpio ◽

Carp Cyprinus Carpio

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Experimental Horizontal Transmission of Enterospora nucleophila (Microsporea: Enterocytozoonidae) in Gilthead Sea Bream (Sparus aurata)

Animals ◽

10.3390/ani11020362 ◽

2021 ◽

Vol 11 (2) ◽

pp. 362

Author(s):

Amparo Picard-Sánchez ◽

M. Carla Piazzon ◽

Itziar Estensoro ◽

Raquel Del Pozo ◽

Nahla Hossameldin Ahmed ◽

...

Keyword(s):

Sparus Aurata ◽

Horizontal Transmission ◽

Infected Fish ◽

Gilthead Sea Bream ◽

Sea Bream ◽

Target Tissue ◽

Farmed Fish ◽

Infection Level ◽

Available Information

Enterospora nucleophila is a microsporidian enteroparasite that infects mainly the intestine of gilthead sea bream (Sparus aurata), leading to an emaciative syndrome. Thus far, the only available information about this infection comes from natural outbreaks in farmed fish. The aim of the present study was to determine whether E. nucleophila could be transmitted horizontally using naturally infected fish as donors, and to establish an experimental in vivo procedure to study this host–parasite model without depending on natural infections. Naïve fish were exposed to the infection by cohabitation, effluent, or intubated either orally or anally with intestinal scrapings of donor fish in four different trials. We succeeded in detecting parasite in naïve fish in all the challenges, but the infection level and the disease signs were always milder than in donor fish. The parasite was found in peripheral blood of naïve fish at 4 weeks post-challenge (wpc) in oral and effluent routes, and up to 12 wpc in the anal transmission trial. Molecular diagnosis detected E. nucleophila in other organs besides intestine, such as gills, liver, stomach or heart, although the intensity was not as high as in the target tissue. The infection tended to disappear through time in all the challenge routes assayed, except in the anal infection route.

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