3.P.219 Laminar shear stress induced gene expression of a monocyte adhesion molecule, GRO, in endothelial cells

SummaryMicroRNAs (miRs) are small non-coding RNAs that control gene expression by inhibiting translation or inducing degradation of targeted mRNA. miRs play a crucial role in vascular homeostasis but also during pathophysiological processes. Functionally active endothelial cells maintain homeostasis of the vasculature and protect against cardiovascular disease. The mechanical activation of endothelial cells by laminar shear stress provides a potent atheroprotective effect and reduces endothelial inflammation and cell cycle progression. Laminar shear stress induces profound changes in gene expression and recently was shown to regulate various miRs. The down-regulation of miR-92a by shear stress enhances the expression of the endothelial nitric oxide synthase, whereas the up-regulation of miR-19a contributes to the shear stress-induced inhibition of cell proliferation. In addition, members of the miR-23–27–24 cluster are increased and specifically miR-23b blocks cell cycle progression, whereas miR-27b was shown to reduce endothelial cell repulsive signals. Finally, increased miR-10 expression in atheroprotected regions reduced the inflammatory response of endothelial cells and increased endothelial miR-143/145 levels improved smooth muscle cells functions. Together, the regulation of miRs by shear stress contributes to the anti-inflammatory, cell cycle inhibitory and vasculoprotective effects in endothelial cells.

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Laminar shear stress prevents simvastatin-induced adhesion molecule expression in cytokine activated endothelial cells

European Journal of Pharmacology ◽

10.1016/j.ejphar.2010.09.016 ◽

2010 ◽

Vol 649 (1-3) ◽

pp. 268-276 ◽

Cited By ~ 9

Author(s):

Joanna Rossi ◽

Leonie Rouleau ◽

Alexander Emmott ◽

Jean-Claude Tardif ◽

Richard L. Leask

Keyword(s):

Endothelial Cells ◽

Shear Stress ◽

Adhesion Molecule ◽

Adhesion Molecule Expression ◽

Laminar Shear Stress ◽

Laminar Shear

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The Gene Expression of Human Endothelial Cells Is Modulated by Subendothelial Extracellular Matrix Proteins: Short-Term Response to Laminar Shear Stress

Tissue Engineering Part A ◽

10.1089/ten.tea.2013.0153 ◽

2014 ◽

Vol 20 (15-16) ◽

pp. 2253-2264 ◽

Cited By ~ 8

Author(s):

Jaroslav Chlupac ◽

Elena Filova ◽

Jana Havlikova ◽

Roman Matejka ◽

Tomas Riedel ◽

...

Keyword(s):

Gene Expression ◽

Extracellular Matrix ◽

Endothelial Cells ◽

Shear Stress ◽

Matrix Proteins ◽

Laminar Shear Stress ◽

Human Endothelial Cells ◽

Short Term ◽

Laminar Shear ◽

Term Response

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The effect of salvianolic acid B combined with laminar shear stress on TNF-α-stimulated adhesion molecule expression in human aortic endothelial cells

Clinical Hemorheology and Microcirculation ◽

10.3233/ch-2010-1269 ◽

2010 ◽

Vol 44 (4) ◽

pp. 245-258 ◽

Cited By ~ 10

Author(s):

Li Xia Xie ◽

Siva Sundara Kumar Durairajan ◽

Jia Hong Lu ◽

Chen Li Liu ◽

Wan Fung Kum ◽

...

Keyword(s):

Endothelial Cells ◽

Shear Stress ◽

Adhesion Molecule ◽

Salvianolic Acid B ◽

Adhesion Molecule Expression ◽

Laminar Shear Stress ◽

Aortic Endothelial Cells ◽

Tnf Α ◽

Human Aortic Endothelial Cells ◽

Laminar Shear

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Primary cilia of human endothelial cells disassemble under laminar shear stress

The Journal of Cell Biology ◽

10.1083/jcb.200312133 ◽

2004 ◽

Vol 164 (6) ◽

pp. 811-817 ◽

Cited By ~ 145

Author(s):

Carlo Iomini ◽

Karla Tejada ◽

Wenjun Mo ◽

Heikki Vaananen ◽

Gianni Piperno

Keyword(s):

Endothelial Cells ◽

Shear Stress ◽

Primary Cilia ◽

Umbilical Vein ◽

Intraflagellar Transport ◽

Mechanical Stimuli ◽

Laminar Shear Stress ◽

Human Endothelial Cells ◽

Umbilical Vein Endothelial Cells ◽

Laminar Shear

We identified primary cilia and centrosomes in cultured human umbilical vein endothelial cells (HUVEC) by antibodies to acetyl-α-tubulin and capillary morphogenesis gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/centriole at interphase and mitosis. To study the response of primary cilia and centrosomes to mechanical stimuli, we exposed cultured HUVEC to laminar shear stress (LSS). Under LSS, all primary cilia disassembled, and centrosomes were deprived of CMG-1. We conclude that the exposure to LSS ends the IFT in cultured endothelial cells.

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