THE FRACTIONATION OF CATTLE BLOOD IODINE WITH ALCOHOL

ABSTRAK Gen FTO berfungsi sebagai regulasi homeostasis, deposisi lemak dan pengaturan obesitas. Penelitian ini bertujuan untuk mengidentifikasi polimorfisme SNP g.125550A>T di ekson 3 gen FTO pada bangsa sapi potong Indonesia. Sampel darah diperoleh dari 209 ekor sapi, terdiri atas sapi bali (44), madura (20), Pesisir (20), katingan (20), Peranakan ongole (PO) (22), Pasundan (20), Sumba Ongole (SO) (11), brahman (20), simental (15), dan limousin (18). Polimorfisme gen FTO dianalisis menggunakan metode PCR-RFLP (HpyCH4III) dan direct sequencing. Hasil genotiping SNP g.125550A>T adalah polimorfik (genotipe AA, AT, dan TT) pada sapi madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, dan limousin. Frekuensi alel A dan T masing-masing adalah 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 dan 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31. Nilai Ho dan He masing-masing adalah 0,60-0,14 dan 0,44-0,18 serta dalam keseimbangan Hardy-Weinberg (P>0.05). Sementara pada sapi bali bersifat monomorfik hanya bergenotipe AA. Hasil sekuensing SNP g.125550A>T ditemukan mutasi tranvesi A menjadi T pada posisi nukleotida g.125550. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa SNP 125550A>T gen FTO beragam dan berpotensi dijadikan marka genetik untuk kualitas daging pada bangsa sapi potong Indonesia.Kata Kunci: gen FTO, PCR-RFLP, Sapi, SNP g.125550A>TABSTRACTThe FTO gene functions as regulation of homeostasis, fat deposition and regulation of obesity. This study aimed to identify the polymorphism of SNP g.125550A>T in exon 3 of FTO gene in Indonesian beef cattle. Blood samples were collected from 209 cattle, including bali (44), madura (20), pesisir (20), katingan (20), PO (22), pasundan (20), SO (11), brahman (20), simental (15), and limousin (18). Polymorphism of the FTO gene was analyzed using PCR-RFLP (HpyCH4III) and direct sequencing methods. The results of genotyping SNP g.125550A>T was polymorphic (AA, AT and TT genotypes) in madura, pesisir, katingan, PO, pasundan, SO, brahman, simental, and limousin cattle. The frequency of A and T alleles were 0,70, 0,68, 0,84, 0,89, 0,70, 0,86, 0,90, 0,73, 0,69 and 0,30, 0,33, 0,16, 0,11, 0,30, 0,14, 0,10, 0,27, 0,31 respectively. The values of Ho and He were 0,60-0,14 and 0,44-0,18 respectively and in Hardy-Weinberg equilibrium (P>0,05). While in Bali cattle was monomorphic (AA genotype). Results of sequencing SNP g.125550A>T of the FTO gene found a transverse mutation A to T at the nucleotide position g.125550. As a result of this study, it can be concluded that SNP 125550A>T of the FTO gene was diverse and potentially used as genetic markers for meat quality in Indonesian beef cattle.Keywords: cattle, FTO gene, PCR-RFLP, SNP g.125550A>T.

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Determination of Mycoplasma bovis specific antibodies in blood sera of asymptomatic carriers-calves in three farms in the Republic of Serbia by using indirect ELISA assay

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.15518 ◽

2017 ◽

Vol 65 (2) ◽

pp. 79

Author(s):

D. VOJINOVIĆ ◽

A. VASIĆ ◽

J. ŽUTIĆ ◽

B. DURIČIĆ ◽

Z. ILIĆ ◽

...

Keyword(s):

Indirect Elisa ◽

Mycoplasma Bovis ◽

Elisa Assay ◽

Serum Samples ◽

Asymptomatic Carriers ◽

Elisa Kit ◽

Cattle Blood ◽

The Republic ◽

The Individual

Blood serum samples of asymptomatic carriers-calves were collected from three farms in the territory of the Republic of Serbia during 2011 and 2012. Commercial Mycoplasma bovis ELISA kit (Bio-X Diagnostics, Belgique) for serological diagnosis from cattle blood sera and milk was used in this research. Calves’ blood sera were tested using immunoenzymatic indirect ELISA assay as described by manufacturer’s instructions. From 5603 blood sera of asymptomatic carriers-calves 144 (2,57%) samples were tested positive for the presence of specific Mycoplasma bovis antibodies. In three different farms proportions of seropositive samples varied from 0,32% to 10,6% in regard to total number of tested samples from the individual farms. In this paper we present the results of Mycoplasma bovis prevalence in asymptomatic carriers-calves.

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Impact of Tahanids on Cattle: Blood Meal Size and Preferred Feeding Sites12

Journal of Economic Entomology ◽

10.1093/jee/73.3.431 ◽

1980 ◽

Vol 73 (3) ◽

pp. 431-433 ◽

Cited By ~ 25

Author(s):

A. L. Hollander ◽

R. E. Wright

Keyword(s):

Blood Meal ◽

Meal Size ◽

Cattle Blood ◽

Blood Meal Size

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PSIX-19 Effect of substituting groundnut cake with wheat bran mixed with cattle blood meal at ratio 1:1 on performance of broilers

Journal of Animal Science ◽

10.1093/jas/skab235.483 ◽

2021 ◽

Vol 99 (Supplement_3) ◽

pp. 264-264

Author(s):

Foluke A Aderemi

Keyword(s):

Wheat Bran ◽

Blood Meal ◽

Inflammatory Responses ◽

Body Weight Gain ◽

Broiler Chicks ◽

Groundnut Cake ◽

Inclusion Level ◽

Cattle Blood ◽

Kidney Liver ◽

Better Than

Abstract This study investigated substituting groundnut cake with wheat bran mixed with cattle blood at ratio1:1 in broilers and it lasted for eight weeks. Seventy-five broiler chicks were allotted randomly to five treatment replicated thrice. Control had 15% GNC, 0% blood meal wheat bran (BMWB) in diets II -V GNC was replaced with BMWB at graded levels of 25,50,75 and 100%. Results revealed that body weight gain and feed intake of diets II and III were similar and (P = 0.05) higher than other diets at both phases. Feed efficiency of diet II was better than others at the starter phase while diet III was better at finisher phase. Haematology and serum chemistry at the finisher phase showed that heamoglobin, white blood cell lymphocyte heterophil and plasma of the birds fed diets II, III, IV and V were (P < 0.05) higher than those on control. The kidney liver and spleen histopathology revealed hepatic and renal degeneration, necrosis and inflammatory responses in diets IV and V. Carcass characteristics showed the wings shanks drumstick leg heart and spleen of birds on BMWB were higher than control. Conclusively it seems BMWB could replace GNC without adverse effect on the broilers at 25% inclusion level

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Report on the Present Stage of Investigations in the Problem of Cattle Blood Groups in Rumania

Blood Groups of Animals ◽

10.1007/978-94-017-6289-2_53 ◽

1965 ◽

pp. 431-432

Author(s):

V. Derlogea ◽

I. Gavrilet ◽

I. Granciu ◽

Z. Sirbu ◽

I. Soceanu ◽

...

Keyword(s):

Blood Groups ◽

Present Stage ◽

Cattle Blood

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Molecular identification of trypanosomes in cattle in Malawi using PCR methods and nanopore sequencing: epidemiological implications for the control of human and animal trypanosomiases

Parasite ◽

10.1051/parasite/2020043 ◽

2020 ◽

Vol 27 ◽

pp. 46

Author(s):

Megasari Marsela ◽

Kyoko Hayashida ◽

Ryo Nakao ◽

Elisha Chatanga ◽

Alex Kiarie Gaithuma ◽

...

Keyword(s):

Human African Trypanosomiasis ◽

Trypanosoma Congolense ◽

Nanopore Sequencing ◽

African Trypanosomiasis ◽

Trypanosoma Brucei Rhodesiense ◽

Pcr Screening ◽

African Trypanosomes ◽

Cattle Blood ◽

Comparison Of The Results ◽

First Time

This study aimed to identify trypanosomes infecting cattle in Malawi in order to understand the importance of cattle in the transmission dynamics of Human African Trypanosomiasis (HAT) and Animal African Trypanosomosis (AAT). A total of 446 DNA samples from cattle blood from three regions of Malawi were screened for African trypanosomes by ITS1 PCR. The obtained amplicons were sequenced using a portable next-generation sequencer, MinION, for validation. Comparison of the results from ITS1 PCR and MinION sequencing showed that combining the two methods provided more accurate species identification than ITS1 PCR alone. Further PCR screening targeting the serum resistance-associated (SRA) gene was conducted to detect Trypanosoma brucei rhodesiense. Trypanosoma congolense was the most prevalent Trypanosoma sp., which was found in Nkhotakota (10.8%; 20 of 185), followed by Kasungu (2.5%; 5 of 199). Of note, the prevalence of T. b. rhodesiense detected by SRA PCR was high in Kasungu and Nkhotakota showing 9.5% (19 of 199) and 2.7% (5 of 185), respectively. We report the presence of animal African trypanosomes and T. b. rhodesiense from cattle at the human–livestock–wildlife interface for the first time in Malawi. Our results confirmed that animal trypanosomes are important causes of anemia in cattle and that cattle are potential reservoirs for human African trypanosomiasis in Malawi.

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The A System of Cattle Blood Groups

Vox Sanguinis ◽

10.1159/000460733 ◽

1963 ◽

Vol 8 (3) ◽

pp. 289-301

Author(s):

I.J. Sinclair

Keyword(s):

Blood Groups ◽

Cattle Blood

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