scholarly journals Propeptide-mediated regulation of procollagen synthesis in IMR-90 human lung fibroblast cell cultures. Evidence for transcriptional control.

1991 ◽  
Vol 266 (5) ◽  
pp. 2983-2987 ◽  
Author(s):  
C H Wu ◽  
C M Walton ◽  
G Y Wu
Keyword(s):  
Cell Cultures ◽  
Transcriptional Control ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Human Lung Fibroblast

scholarly journals Essential Oil Content of the Rhizome ofCurcuma purpurascensBl. (Temu Tis) and Its Antiproliferative Effect on Selected Human Carcinoma Cell Lines

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Sok-Lai Hong ◽  
Guan-Serm Lee ◽  
Syarifah Nur Syed Abdul Rahman ◽  
Omer Abdalla Ahmed Hamdi ◽  
Khalijah Awang ◽  
...  
Keyword(s):  
Human Lung ◽  
Carcinoma Cell ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Fibroblast Cell Line ◽  
Human Carcinoma ◽  
Human Lung Fibroblast ◽  
Carcinoma Cell Lines ◽  
Lung Fibroblast Cell Line ◽  
Human Carcinoma Cell

Curcuma purpurascensBl., belonging to the Zingiberaceae family, is known astemu tisin Yogyakarta, Indonesia. In this study, the hydrodistilled dried ground rhizome oil was investigated for its chemical content and antiproliferative activity against selected human carcinoma cell lines (MCF7, Ca Ski, A549, HT29, and HCT116) and a normal human lung fibroblast cell line (MRC5). Results from GC-MS and GC-FID analysis of the rhizome oil oftemu tisshowed turmerone as the major component, followed by germacrone,ar-turmerone, germacrene-B, and curlone. The rhizome oil oftemu tisexhibited strong cytotoxicity against HT29 cells (IC50value of 4.9 ± 0.4 μg/mL), weak cytotoxicity against A549, Ca Ski, and HCT116 cells (with IC50values of 46.3 ± 0.7, 32.5 ± 1.1, and 35.0 ± 0.3 μg/mL, resp.), and no inhibitory effect against MCF7 cells. It exhibited mild cytotoxicity against a noncancerous human lung fibroblast cell line (MRC5), with an IC50value of 25.2 ± 2.7 μg/mL. This is the first report on the chemical composition of this rhizome’s oil and its selective antiproliferative effect on HT29. The obtained data provided a basis for further investigation of the mode of cell death.

Histamine and Serotonin Stimulate Eotaxin Production by a Human Lung Fibroblast Cell Line

2002 ◽  
Vol 128 (Suppl. 1) ◽  
pp. 12-17 ◽  
Author(s):  
Etsuro Sato ◽  
Masayuki Haniuda ◽  
Hiroki Numanami ◽  
Toshiki Ushiyama ◽  
Akihiro Tsukadaira ◽  
...  
Keyword(s):  
Cell Line ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Fibroblast Cell Line ◽  
Human Lung Fibroblast ◽  
Lung Fibroblast Cell Line

PROLIFERATIVE ACTIVITY OF SAPONIN-REDUCING Carica papaya LEAVES EXTRACTS ON HUMAN LUNG FIBROBLAST CELL (IMR90)

2016 ◽  
Vol 78 (11-3) ◽  
Author(s):  
Noraziani Zainal Abidin ◽  
Hazreen Omar ◽  
Anathasia Janam Empungan ◽  
Nurulain ‘Atikah Kamalaldin ◽  
Badrul Hisham Yahaya ◽  
...  
Keyword(s):  
Platelet Count ◽  
Dengue Fever ◽  
Proliferative Activity ◽  
Carica Papaya ◽  
Human Lung ◽  
Antioxidant Properties ◽  
Material Balance ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Human Lung Fibroblast

Carica papaya belongs to Carricaceae family, which has been proven traditionally to treat dengue fever due to its pharmacological properties to increase platelet count. However, during the critical phase of dengue fever, the platelet count will decrease due to the blood vessel rupturing. Therefore, the main objectives of this study were to reduce the bitterness of Carica papaya extract by removing saponin and to study the effect of saponin-reducing extract on the proliferative activity of human lung fibroblast cell (IMR90). For preparative isolation of the saponin compound, peleg model was used to determine the maximum extract concentration, exhaustive time of extraction and total saponin content (TSC) using different weights of dry Amberlite® IRA-67 resin. The remaining saponins in the extract were quantified by mean of RP-HPLC prior to material balance. Then, approximately 1.0 x 104 cells of IMR90 were seeded onto 96-well plate and later treated with various concentrations of extracts for 3 days of incubation. The results showed that, the amount of saponin left in the extract was approximately the same as in the untreated extract (p<0.05). A short adsorption incubation time (2 hrs) was believed to affect the saponin adsorption efficiency. In fact, other bio-active constituents (e.g. polyphenolic compounds) might have been adsorbed as there was a significant depreciation of antioxidant properties on the treated extract (p<0.05). In conclusion, after three consecutively days of extracts-IMR90 cell incubation, the best EC50 values of both untreated and saponin-reducing extracts were observed to be more than 24 hrs of exposure ranging from 104.08 ± 0.90 to 17040.47 ± 2.30 µg/ml. Meanwhile, saponin-reducing extract has been proven not to affect any normal cell growth but in fact it decreased 1.2-fold as compared to the extract containing saponin (control). 

Bradykinin stimulates eotaxin production by a human lung fibroblast cell line

2000 ◽  
Vol 106 (1) ◽  
pp. 117-123 ◽  
Author(s):  
Etsuro Sato ◽  
Dan K. Nelson ◽  
Sekiya Koyama ◽  
Jeffrey C. Hoyt ◽  
Richard A. Robbins
Keyword(s):  
Cell Line ◽  
Human Lung ◽  
Fibroblast Cell ◽  
Lung Fibroblast ◽  
Fibroblast Cell Line ◽  
Human Lung Fibroblast ◽  
Lung Fibroblast Cell Line
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