scholarly journals Transcriptional and post-transcriptional regulation of L-type pyruvate kinase gene expression in rat liver.

1986 ◽  
Vol 261 (17) ◽  
pp. 7621-7625 ◽  
Author(s):  
S Vaulont ◽  
A Munnich ◽  
J F Decaux ◽  
A Kahn
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Pyruvate Kinase ◽  
Rat Liver ◽  
Kinase Gene ◽  
Post Transcriptional Regulation

scholarly journals Post-transcriptional regulation of rat liver gene expression by glucocorticoids

1985 ◽  
Vol 13 (18) ◽  
pp. 6467-6482 ◽  
Author(s):  
Ruth Fulton ◽  
George D. Birnie ◽  
John T. Knowler
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Rat Liver ◽  
Liver Gene ◽  
Post Transcriptional Regulation

scholarly journals Fast and Efficient 5′P Degradome Library Preparation for Analysis of Co-Translational Decay in Arabidopsis

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 466
Author(s):  
Marie-Christine Carpentier ◽  
Cécile Bousquet-Antonelli ◽  
Rémy Merret
Keyword(s):  
Gene Expression ◽  
Quality Control ◽  
Transcriptional Regulation ◽  
High Throughput ◽  
Library Preparation ◽  
Working Day ◽  
Set Up ◽  
Post Transcriptional Regulation ◽  
Commercial Kit

The recent development of high-throughput technologies based on RNA sequencing has allowed a better description of the role of post-transcriptional regulation in gene expression. In particular, the development of degradome approaches based on the capture of 5′monophosphate decay intermediates allows the discovery of a new decay pathway called co-translational mRNA decay. Thanks to these approaches, ribosome dynamics could now be revealed by analysis of 5′P reads accumulation. However, library preparation could be difficult to set-up for non-specialists. Here, we present a fast and efficient 5′P degradome library preparation for Arabidopsis samples. Our protocol was designed without commercial kit and gel purification and can be easily done in one working day. We demonstrated the robustness and the reproducibility of our protocol. Finally, we present the bioinformatic reads-outs necessary to assess library quality control.

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