Spectral properties of trifluoroacetic acid—acetonitrile gradient systems for separation of picomole quantities of peptides by reversed-phase high-performance liquid chromatography

1985 ◽  
Vol 347 ◽  
pp. 83-88 ◽  
Author(s):  
Günther Winkler ◽  
Peter Wolschann ◽  
Peter Briza ◽  
Franz X. Heinz ◽  
Christian Kunz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Trifluoroacetic Acid ◽  
Spectral Properties ◽  
High Performance ◽  
Reversed Phase ◽  
Gradient Systems ◽  
Acetonitrile Gradient

High Perform ance Liquid Chrom atographie Analysis o f Steroidal Saponins from Avena sativa L

1981 ◽  
Vol 36 (11-12) ◽  
pp. 1072-1074 ◽  
Author(s):  
J. Kesselmeier ◽  
D. Strack
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Avena Sativa ◽  
High Performance ◽  
Reversed Phase ◽  
Detector Response ◽  
Gradient System ◽  
Steroidal Saponins ◽  
Uv Detector ◽  
Acetonitrile Gradient

Abstract Reversed phase high performance liquid chromatography offers an efficient and rapid method for analysis of steroidal saponins. Crude extracts from primary leaves of Avena sativa and isolated etioplasts therefrom have been resolved into four saponins (avenacosides) using a water-acetonitrile gradient system on RP-8 and monitoring the column effluent at 200 nm with an UV-detector. Detectability was found to be in the range of 50 ng avenacoside B and the detector response was linear up to 8 μg tested. The described method is applicable to studies on localization and physiology of Avena saponins during development of the primary leaf.

Development and Validation of A Reversed Phase-High Performance Liquid Chromatography Method for the Quantitative Estimation of Ramipril Drug Content from Formulated Dosage Form

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
Raju Chandra ◽  
Manisha Pant ◽  
Harchan Singh ◽  
Deepak Kumar ◽  
Ashwani Sanghi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reversed Phase ◽  
Uv Detector ◽  
Chromatography Method ◽  
Drug Content

A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of Remipril drug content from marketed bulk tablets. The active ingredient of Remipril separation achieved with C18 column using the methanol water mobile phase in the ratio of 40:60 (v/v). The active ingredient of the drug content quantify with UV detector at 215 nm. The retention time of Remipril is 5.63 min. A good linearity relation (R2=0.999) was obtained between drug concentration and average peak areas. The limit of detection and limit of quantification of the instrument were calculated 0.03 and 0.09 µg/mL, respectively. The accuracy of the method validation was determined 102.72% by recoveries method.

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