RNA- and protein-synthesis inhibitors induce apoptosis in a midgut cell line from the spruce budworm, Choristoneura fumiferana

1996 ◽  
Vol 42 (11-12) ◽  
pp. 1061-1069 ◽  
Author(s):  
S.R. Palli ◽  
S.S. Sohi ◽  
B.J. Cook ◽  
A.J. Brownwright ◽  
G.F. Caputo ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Cell Line ◽  
Choristoneura Fumiferana ◽  
Spruce Budworm ◽  
Protein Synthesis Inhibitors ◽  
Midgut Cell ◽  
Rna And Protein Synthesis

scholarly journals Induction of N-acetylglucosamine kinase in yeast

1974 ◽  
Vol 141 (2) ◽  
pp. 593-595 ◽  
Author(s):  
A. Bhattacharya ◽  
M. Puri ◽  
A. Datta
Keyword(s):  
Protein Synthesis ◽  
Enzyme Activity ◽  
Candida Albicans ◽  
Enzyme Synthesis ◽  
Transcriptional Level ◽  
Protein Synthesis Inhibitors ◽  
Rna And Protein Synthesis ◽  
New Protein

The presence of N-acetylglucosamine is essential for the induced synthesis of N-acetylglucosamine kinase in Candida albicans. The enzyme synthesis stops and its concentration in the cells declines rapidly as soon as N-acetylglucosamine is removed from the medium. Experiments with RNA- and protein-synthesis inhibitors indicate that the appearance of new enzyme activity is dependent on concomitant new protein synthesis and the inducer operates at a transcriptional level.

Metabolic requirements for interactions between nuclear and cytoplasmic membranes in the repair of damaged Amoeba nuclei

1976 ◽  
Vol 21 (2) ◽  
pp. 291-302
Author(s):  
C.J. Flickinger
Keyword(s):  
Endoplasmic Reticulum ◽  
Protein Synthesis ◽  
Membrane Fusion ◽  
Energy Production ◽  
Rna Synthesis ◽  
Metabolic Inhibitors ◽  
Protein Synthesis Inhibitors ◽  
Nuclear Membranes ◽  
Cytoplasmic Membranes ◽  
Rna And Protein Synthesis

Amoeba nuclear envelopes were damaged using microsurgery, and metabolic requirements for the steps in their repair were studied, and my placing the cells in a solution containing one of several metabolic inhibitors. The first step in repair, the association of pieces of endoplasmic reticulum with holes in the nuclear membranes, appears to be a passive process since it was not affected by inhibitors of energy production, RNA synthesis, or protein synthesis. In contrast, fusion of pieces of endoplasmic reticulum with the nuclear membranes at the margins of the holes was blocked by KCN and dinitrophenol, indicating that membrane fusion requires energy derived from respiration, but RNA and protein synthesis inhibitors did not prevent fusion of pieces of endoplasmic reticulum with the nuclear membranes. The subsequent completion of repair and restoration of intact nuclear membranes was almost completely blocked by inhibitors of respiration, and it was reduced in the presence of actinomycin and emetine, suggesting that in addition to a requirement for energy, some later steps in the repair of the nuclear membranes require RNA and protein synthesis.

Ultrastructural investigation of the effect of DNA, RNA, and protein synthesis inhibitors on ribosome crystallization

1973 ◽  
Vol 44 (3-4) ◽  
pp. 265-278 ◽  
Author(s):  
Nadir M. Maraldi ◽  
Graziella Biagini ◽  
Paolo Simoni ◽  
Marcello Barbieri ◽  
Marina Marini ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Protein Synthesis Inhibitors ◽  
Ultrastructural Investigation ◽  
Rna And Protein Synthesis

Baculovirus ACMNPV as well as RNA- and protein-synthesis inhibitors induce apoptosis in a continuous midgut cell line, FPMI-CF-203, of Choristonuera fumiferana (Lepitoptera: tortricidae)

1995 ◽  
Vol 53 ◽  
pp. 1030-1031
Author(s):  
A.J. Brownwright ◽  
S.R. Palli ◽  
G.F. Caputo ◽  
S.S. Sohi
Keyword(s):  
Protein Synthesis ◽  
Nuclear Polyhedrosis Virus ◽  
Choristoneura Fumiferana ◽  
Protein Synthesis Inhibitor ◽  
Post Inoculation ◽  
Protein Synthesis Inhibitors ◽  
Synthesis Inhibitor ◽  
Successful Infection ◽  
Polyhedrosis Virus ◽  
Nuclear Polyhedrosis

Apoptosis is an active cellular self-destruction regulated by expression or repression of certain genes. Apoptosis can be caused by a variety of both external and internal stimuli. Whether these different stimuli that can cause apoptosis converge into a final pathway that leads to self-destruction is not known. This paper compares apoptosis caused by a baculovirus, an RNA-synthesis inhibitor and a protein-synthesis inhibitor.Inoculation of IPLB-SF-21 (SF-21) and FPMI-CF-203 (CF-203, Fig. 1, ) cells with Autographa californica multicapsid nuclear polyhedrosis virus (AcMNPV) and Choristoneura fumiferana multicapsid nuclear polyhedrosis virus (CfMNPV), respectively, results in successful infection as visualized by formation of occlusion bodies (OBs). However, inoculation of CF-203 cells with AcMNPV, or SF-21 cells with CFMNPV, is unsuccessful and no OBs are seen. Inoculation of CF-203 cells with AcMNPV results in premature lysis of cells beginning at 12 hr post-inoculation (pi) and most of the cells are lysed by 48 hr pi.

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