scholarly journals Tauro-7 alpha, 12 alpha-dihydroxy-5 beta-cholanic acid as internal standard in the gas-liquid chromatographic analysis of bile acid methyl ester acetates.

1983 ◽  
Vol 24 (10) ◽  
pp. 1376-1379
Author(s):  
Y Ghoos ◽  
P Rutgeerts ◽  
G Vantrappen
Keyword(s):  
Bile Acid ◽  
Methyl Ester ◽  
Chromatographic Analysis ◽  
Acid Methyl Ester ◽  
Internal Standard ◽  
Acid Methyl ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Cholanic Acid

scholarly journals Planar Chromatographic and Liquid Chromatographic Analysis of Thiamphenicol in Bovine and Human Plasma

1997 ◽  
Vol 80 (4) ◽  
pp. 746-750 ◽  
Author(s):  
Elena Dreassi ◽  
Gianfranco Corbini ◽  
Vittoria Ginanneschi ◽  
Piero Corti ◽  
Sandra Furlanetto
Keyword(s):  
Human Plasma ◽  
Chromatographic Analysis ◽  
Extraction Method ◽  
Chromatographic Method ◽  
Internal Standard ◽  
Bovine Plasma ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Pc Method

Abstract A planar chromatographic (PC) method was developed to determine thiamphenicol in human and bovine plasma with florphenicol as internal standard. The performance of the method was compared with that of a liquid chromatographic method. Recovery of extraction method developed for plasma was 81.51 ± 2.85%. Reproducibilities of the PC assay performed with various PC plates were also good.

Liquid chromatographic analysis of disopyramide and its mono-N-dealkylated metabolite.

1979 ◽  
Vol 25 (3) ◽  
pp. 405-408 ◽  
Author(s):  
J J Lima
Keyword(s):  
Chromatographic Analysis ◽  
Mobile Phase ◽  
High Performance ◽  
Antiarrhythmic Drugs ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Coefficients Of Variation ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic ◽  
Lower Limits

Abstract We describe a rapid, sensitive, and specific "high performance" liquid chromatographic analysis for disopyramide and its mono-N-dealkylated metabolite in serum, urine, and saliva. We used a mu-Bondapak CN column and an acetate buffer mobile phase containing methanol. Retention times for the two compounds and the internal standard, p-chlorodisopyramide, were 3.4, 4.1, and 6.3 min, respectively. The lower limits of sensitivity for drug and metabolite were 50 and 80 micrograms/L, respectively, with maximum coefficients of variation of 4.6 and 12%, respectively. Currently used antiarrhythmic drugs did not interfere with the analysis of disopyramide, and the pharmacokinetics of the drug, obtained from studies of one subject, agree well with reported values.

Liquid-chromatographic analysis for methylphenidate (Ritalin) in serum.

1979 ◽  
Vol 25 (3) ◽  
pp. 401-404 ◽  
Author(s):  
S J Soldin ◽  
Y P Chan ◽  
B M Hill ◽  
J M Swanson
Keyword(s):  
Chromatographic Analysis ◽  
High Performance ◽  
Plasma Sample ◽  
Potassium Phosphate ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Column Temperature ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract We describe a "high performance" liquid chromatographic method for quantitating methylphenidate in serum. The internal standard, 4,5-diphenylimidazole, and serum or plasma sample are extracted in chloroform, evaporated, and redissolved in 20 mmol/L potassium phosphate (pH 3.5)/high-purity acetonitrile, 80/20 by vol. A centrifuged aliquot is chromatographed on mu-Bondapak C-18 with the phosphate/acetonitrile solvent as mobile phase, a flow rate of 1.6 mL/min, and a column temperature of 40 degrees C. Absorbances are read at 192 nm. This method reliably measures concentrations greater than 20 micrograms/L and has analytical recoveries of 74%.

Simultaneous very fast liquid-chromatographic analysis of ethosuximide, primidone, phenobarbital, phenytoin, and carbamazepine in serum.

1983 ◽  
Vol 29 (3) ◽  
pp. 473-476 ◽  
Author(s):  
P M Kabra ◽  
M A Nelson ◽  
L J Marton
Keyword(s):  
Particle Size ◽  
Flow Rate ◽  
Chromatographic Analysis ◽  
Mobile Phase ◽  
Internal Standard ◽  
Reversed Phase ◽  
Ultraviolet Detector ◽  
Analytical Recovery ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

Abstract We describe a sensitive, specific, and very fast liquid-chromatographic assay for simultaneously determining five anticonvulsants (ethosuximide, primidone, phenobarbital, phenytoin, and carbamazepine) by using commercially available 5- or 3-microns particle size reversed-phase columns and a microflow-cell-equipped ultraviolet detector. The anticonvulsant drugs are extracted from 200 microL of serum containing 50 mg of cyclopal per liter as an internal standard, by elution from a Bond-Elut (Analytichem International, Harbor City, CA 90710) column with 300 microL of methanol. A 5-microL aliquot of the eluate is applied to an analytical column and eluted with a mobile phase of acetonitrile/methanol/phosphate buffer, 20 mmol/L, pH 3.7 (13.5/35/51.5 by vol), at a flow rate of 3.0 mL/min and at 50 degrees C. Detection is at 210 or 195 nm. The chromatography is complete in less than 2.5 min with the 5-microns-particle column, and in less than 1.4 min with the 3-microns-particle column. The sensitivity of the method for all drugs is less than 1 mg/L. Analytical recovery of drugs added to serum ranged from 92 to 109% for concentrations up to 200 mg/L. Between-run precision (CV) ranged from 1.3 to 4.1%.

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