scholarly journals Automated method for the colorimetric determination of acyl esters in serum

1965 ◽  
Vol 6 (2) ◽  
pp. 301-306
Author(s):  
Arnold Antonis ◽  
D.S. Platt ◽  
J.M. Thorp
Keyword(s):  
Colorimetric Determination ◽  
Automated Method

An improved semi-automated method for the colorimetric determination of triglycerides in serum

1975 ◽  
Vol 64 (1) ◽  
pp. 45-50 ◽  
Author(s):  
P.N.M. Demacker ◽  
J.B.H.A. Van Oppenraay ◽  
H. Baadenhuijsen ◽  
A.P. Jansen
Keyword(s):  
Colorimetric Determination ◽  
Automated Method

Automated Determination of Serum Glutamic Oxaloacetic Transaminase

1966 ◽  
Vol 12 (2) ◽  
pp. 95-111 ◽  
Author(s):  
Stanley Morgenstern ◽  
Morris Oklander ◽  
Joseph Auerbach ◽  
James Kaufman ◽  
Bernard Klein
Keyword(s):  
Excellent Agreement ◽  
Diazonium Salt ◽  
Colorimetric Determination ◽  
Glutamic Oxaloacetic Transaminase ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Oxaloacetic Acid ◽  
Spectrophotometric Procedure ◽  
Automated Method ◽  
Automated Determination

Abstract A simplified and precise automated method is described for the colorimetric determination of serum glutamic oxaloacetic transaminase. The enzymically generated oxaloacetic acid is dialyzed, coupled with Fast Ponceau L, a diazonium salt, to form a colored solution whose absorbance is measured at 455 mµ. Dialysis eliminates the need for a serum control determination, thereby halving the analysis time, which is usually 1 hr. per 40 specimens. Comparison of values obtained using this procedure with and without dialysis shows excellent agreement. Comparison with the spectrophotometric procedure shows good correlation. The results of sample interaction experiments are also presented.

Automated Method for the Determination of Serum Creatine Kinase Activity

1967 ◽  
Vol 13 (3) ◽  
pp. 233-241 ◽  
Author(s):  
Gerard A Fleisher
Keyword(s):  
Creatine Kinase ◽  
Alkaline Solution ◽  
Kinase Activity ◽  
Adenosine Diphosphate ◽  
Creatine Phosphate ◽  
Creatine Kinase Activity ◽  
Serum Creatine Kinase ◽  
Colorimetric Determination ◽  
Automated Method

Abstract An automated (AutoAnalyzer) method for the colorimetric determination of creatine kinase activity in serum is described. This method includes reactivation of creatine kinase with cysteine, incubation of the active enzyme with creatine phosphate and adenosine diphosphate at 37.5°, and subsequent inactivation of enzyme and binding of cysteine by phenylmercuric borate. The enzymatically produced creatine is dialyzed against a solution of diacetyl and reacted with α-naphthol in an alkaline solution. The absorbance of the colored end product is measured at 550 mµ. Individual blanks are determined in the absence of adenosine diphosphate. Comparison of results obtained by this method and a manual procedure shows satisfactory agreement.

Automated Colorimetric Determination of Phenylephrine Hydrochloride in Drug Formulations. II. Collaborative Study

1971 ◽  
Vol 54 (3) ◽  
pp. 600-602
Author(s):  
Michel Margosis
Keyword(s):  
Coefficient Of Variation ◽  
Statistical Evaluation ◽  
Collaborative Study ◽  
Colorimetric Method ◽  
Colorimetric Determination ◽  
Good Recovery ◽  
Drug Formulations ◽  
Phenylephrine Hydrochloride ◽  
Automated Method

Abstract The automated colorimetric method designed by Lane for the analysis of phenylephrine HCl in drug formulations has been submitted to a collaborative study. Five samples, including 2 with known interferences, were sent to 9 collaborators. The results were subjected to statistical evaluation. These show good recovery and a maximum interlaboratory coefficient of variation of 4.5%. The automated method as evaluated in this study has been adopted as official first action.

A Method for the Colorimetric Determination of β-Glucuronidase in Urine, Serum, Tissue; Assay of Enzymatic Activity in Health and Disease

1959 ◽  
Vol 36 (2) ◽  
pp. 193-201 ◽  
Author(s):  
Julius A. Goldbarg ◽  
Esteban P. Pineda ◽  
Benjamin M. Banks ◽  
Alexander M. Rutenburg
Keyword(s):  
Enzymatic Activity ◽  
Colorimetric Determination ◽  
Health And Disease ◽  
Urine Serum

Colorimetric Determination of Fluoride in Drinking Groundwater using a Polymeric Zirconium Complex of 5-(2-Carboxyphenylazo)-8-Hydroxyquinoline

2013 ◽  
Vol 12 (7) ◽  
pp. 460-465
Author(s):  
Sameer Amereih ◽  
Zaher Barghouthi ◽  
Lamees Majjiad
Keyword(s):  
Drinking Water ◽  
Detection Limit ◽  
Complex Formation ◽  
Molar Absorptivity ◽  
Colorimetric Determination ◽  
Zirconium Complex ◽  
Reliable Determination ◽  
Percentage Recovery ◽  
Quantitation Limit

A sensitive colorimetric determination of fluoride in drinking water has been developed using a polymeric zirconium complex of 5-(2-Carboxyphenylazo)-8-Hydroxyquinoline as fluoride reagents. The method allowed a reliable determination of fluoride in range of (0.0-1.5) mg L-1. The molar absorptivity of the complex formation is 7695 ± 27 L mol-1 cm-1 at 460 nm. The sensitivity, detection limit, quantitation limit, and percentage recovery for 1.0 mg L-1 fluoride for the proposed method were found to be 0.353 ± 0.013 μg mL-1, 0.1 mg L-1, 0.3 mg L-1, and 101.7 ± 4.1, respectively.

Colorimetric Determination of Amoxicillin in Pure and some Pharmaceutical Formulations Via Reaction with Potassium Permanganate as Oxidant Reagent

2019 ◽  
Vol 10 (02) ◽  
Author(s):  
Abbas Shebeeb Al-kadumi ◽  
Sahar Rihan Fadhel ◽  
Mohammed Abdullah Ahmed ◽  
Luma Amer Musa
Keyword(s):  
Potassium Permanganate ◽  
Pharmaceutical Preparations ◽  
Pharmaceutical Formulations ◽  
Atomic Emission ◽  
Basic Medium ◽  
Photometric Method ◽  
Colorimetric Determination ◽  
Spectrophotometric Methods ◽  
Label Claim

We proposed two simple, rapid, and convenient spectrophotometric methods are described for the determination of Amoxicillin in bulk and its pharmaceutical preparations. They are based on the measurement of the flame atomic emission of potassium ion (in first method) and colorimetric determination of the green colored solution for manganite ion at 610 nm formed after reaction of Amoxicillin with potassium permanganate as oxidant agent (in the second method) in basic medium. The working conditions of the methods were investigated and optimized. Beer's law plot showed a good correlation in the concentration range of 5-45 μg/ml. The detection limits and relative standared deviations were (2.573, 2.814 μg/ml) (2.137, 2.498) for the flame emission photometric method and (1.844, 2.016 μg/ml) (1.645,1.932) for colorimetric methods for capsules and suspensions respectively. The methods were successfully applied to the determination of Amoxicillin in capsules and suspensions, and the obtained results were in good agreement with the label claim. No interference was observed from the commonly encountered additives and expectancies.

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