High hydrostatic pressure effect on fluorescence lifetime and spectra for Sm2+-doped SrFCl0.5Br0.5 mixed crystals

2000 ◽  
Vol 92 (1-2) ◽  
pp. 161-164 ◽  
Author(s):  
B.R. Jovanić
Keyword(s):  
Hydrostatic Pressure ◽  
Fluorescence Lifetime ◽  
High Hydrostatic Pressure ◽  
Pressure Effect ◽  
Mixed Crystals

Effect of high hydrostatic pressure on the enzymic hydrolysis of β-lactoglobulin B by trypsin, thermolysin and pepsin

1996 ◽  
Vol 63 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Henrik Stapelfeldt ◽  
Per Hjort Petersen ◽  
Kristian Rotvig Kristiansen ◽  
Karsten Bruun Qvist ◽  
Leif H. Skibsted
Keyword(s):  
High Pressure ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Pressure Effect ◽  
Ambient Pressure ◽  
Apparent Volume ◽  
Enzymic Hydrolysis ◽  
Ambient Conditions ◽  
Β Lactoglobulin ◽  
Hydrolysis Of

SummaryHydrolysis of β-lactoglobulin B (β-lg B) by pepsin, a process slow at ambient conditions, is facilitated at a moderately high hydrostatic pressure such as 300 MPa, corresponding to an apparent volume of activation ΔV# = −63 ml mol−1 at pH 2·5, 30 °C and Γ/2=0·16. Digestion of β-lg by trypsin and thermolysin is likewise enhanced by pressure, and the pressure effect has been traced to pressure denaturation of β-lg B, which by high-pressure fluorescence spectroscopy has been shown to have a large negative volume of reaction, ΔV° = −98 ml mol−1, at pH 6·7, 30 °C and Γ/2 = 0·16. Pressure denaturation is only slowly reversed following release of pressure and the enhanced digestibility is maintained at ambient pressure for several hours.

Evaluation of high hydrostatic pressure effect on human adenovirus using molecular methods and cell culture

2012 ◽  
Vol 157 (3) ◽  
pp. 368-374 ◽  
Author(s):  
Katarina Kovač ◽  
Martijn Bouwknegt ◽  
Marta Diez-Valcarce ◽  
Peter Raspor ◽  
Marta Hernández ◽  
...  
Keyword(s):  
Cell Culture ◽  
Hydrostatic Pressure ◽  
High Hydrostatic Pressure ◽  
Pressure Effect ◽  
Human Adenovirus ◽  
Molecular Methods
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