COX-2 CO-LOCALIZES WITH VIMENTIN AND c-KIT IN BLADDER INTERSTITIAL CELLS AND CAUSES DETRUSOR SMOOTH MUSCLE SPONTANEOUS TONE BY GENERATING PGE2

2008 ◽  
Vol 179 (4S) ◽  
pp. 130-130
Author(s):  
Clinton W Collins ◽  
Adam P Klausner ◽  
Amy Miner ◽  
Benjamin W Herrick ◽  
Harry P Koo ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Interstitial Cells ◽  
Detrusor Smooth Muscle ◽  
Spontaneous Tone ◽  
Cox 2

scholarly journals Expression of cyclooxygenase-2 in urinary bladder in rats with cyclophosphamide-induced cystitis

2007 ◽  
Vol 293 (2) ◽  
pp. R677-R685 ◽  
Author(s):  
Mary Beth Klinger ◽  
Abbey Dattilio ◽  
Margaret A. Vizzard
Keyword(s):  
Smooth Muscle ◽  
Urinary Bladder ◽  
Western Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Inflammatory Cell ◽  
Nerve Fibers ◽  
Cyclooxygenase 2 ◽  
Detrusor Smooth Muscle ◽  
Cox 2

These studies examined the expression of cyclooxygenase-2 (COX-2) expression in the urothelium and suburothelial space and detrusor from rats treated with cyclophosphamide (CYP) to induce acute (4 h), intermediate (48 h), or chronic (10-day) cystitis. Western blot analysis and immunohistochemistry were used to demonstrate COX-2 expression. In whole mount preparations of urinary bladder, nerve fibers in the suburothelial plexus, and inflammatory cell infiltrates were characterized for COX-2 expression after CYP-induced cystitis. COX-2 expression significantly ( P ≤ 0.01) increased in the urothelium + suburothelium and detrusor smooth muscle with acute, intermediate, and chronic (10-day) CYP-induced cystitis, but expression in urothelium + suburothelium was significantly greater. CYP-induced upregulation of COX-2 showed by immunostaining in the urothelium + suburothelium was similar to that observed with Western blot analysis and also demonstrated COX-2 inflammatory cell infiltrates (CD86+) and nerve fibers (PGP+) in the suburothelial plexus. Although COX-2 expression was significantly ( P ≤ 0.01) increased in detrusor smooth muscle, immunohistochemistry failed to demonstrate an obvious change in COX-2-immunoreactivity (IR) in detrusor muscle, but COX-2 inflammatory infiltrates were present throughout the detrusor. COX-2-IR nerve fibers exhibited increased density in the suburothelial plexus with acute or chronic CYP-induced cystitis. COX-2-IR macrophages (CD86+) were present throughout the urinary bladder with acute and chronic CYP-induced cystitis. These studies demonstrate cellular targets in the urinary bladder where COX-2 inhibitors may act.

257: The Phosphodiesterase Type 4 Inhibitor, Rolipram, is More Efficient to Relax Detrusor Smooth Muscle in Rats with Overactive Bladder than in Control Rats

2007 ◽  
Vol 177 (4S) ◽  
pp. 86-86
Author(s):  
Stephanie Oger ◽  
Delphine Behr-Roussel ◽  
Katell Mevel ◽  
Jacques Bernabe ◽  
Pierre Denys ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Overactive Bladder ◽  
Detrusor Smooth Muscle ◽  
Phosphodiesterase Type

373: The Contractile Properties of Detrusor Smooth Muscle in Cloacal Exstrophy

2007 ◽  
Vol 177 (4S) ◽  
pp. 124-124
Author(s):  
Navroop S. Johal ◽  
Peter M. Cuckow ◽  
Christopher H. Fry
Keyword(s):  
Smooth Muscle ◽  
Contractile Properties ◽  
Detrusor Smooth Muscle ◽  
Cloacal Exstrophy

Sorghum Fermented by Aspergillus oryzae NK Enhances Inhibition of Vascular Inflammation in TNF-α-stimulated Human Aortic Smooth Muscle Cells

2018 ◽  
Vol 88 (5-6) ◽  
pp. 309-318
Author(s):  
Hae Seong Song ◽  
Jung-Eun Kwon ◽  
Hyun Jin Baek ◽  
Chang Won Kim ◽  
Hyelin Jeon ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Inflammatory Response ◽  
Smooth Muscle Cells ◽  
Adhesion Molecule ◽  
Vascular Inflammation ◽  
Muscle Cells ◽  
Aortic Smooth Muscle Cells ◽  
Aortic Smooth Muscle ◽  
Tnf Α ◽  
Cox 2

Abstract. Sorghum bicolor L. Moench is widely grown all over the world for food and feed. The effects of sorghum extracts on general inflammation have been previously studied, but its anti-vascular inflammatory effects are unknown. Therefore, this study investigated the anti-vascular inflammation effects of sorghum extract (SBE) and fermented extract of sorghum (fSBE) on human aortic smooth muscle cells (HASMCs). After the cytotoxicity test of the sorghum extract, a series of experiments were conducted. The inhibition effects of SBE and fSBE on the inflammatory response and adhesion molecule expression were measured using treatment with tumor necrosis factor-α (TNF-α), a crucial promoter for the development of atherosclerotic lesions, on HASMCs. After TNF-α (10 ng/mL) treatment for 2 h, then SBE and fSBE (100 and 200 μg/mL) were applied for 12h. Western blotting analysis showed that the expression of vascular cell adhesion molecule-1 (VCAM-1) (2.4-fold) and cyclooxygenase-2 (COX-2) (6.7-fold) decreased, and heme oxygenase-1 (HO-1) (3.5-fold) increased compared to the TNF-α control when treated with 200 μg/mL fSBE (P<0.05). In addition, the fSBE significantly increased the expression of HO-1 and significantly decreased the expression of VCAM-1 and COX-2 compared to the TNF-α control in mRNA level (P<0.05). These reasons of results might be due to the increased concentrations of procyanidin B1 (about 6-fold) and C1 (about 30-fold) produced through fermentation with Aspergillus oryzae NK for 48 h, at 37 °C. Overall, the results demonstrated that fSBE enhanced the inhibition of the inflammatory response and adherent molecule expression in HASMCs.

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