75. Detection of bovine viral diarrhoea virus (BVDV) in bulk milk by a single tube TaqMan RT-PCR

2002 ◽  
Vol 72 ◽  
pp. 27
Author(s):  
G.S. Heath ◽  
D.P. King ◽  
P.R. Wakeley ◽  
M. Banks
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Single Tube ◽  
Bulk Milk ◽  
Diarrhoea Virus

Analysis of bovine viral diarrhoea virus in Korea dairy farms

2016 ◽  
Author(s):  
Hong-Je Park ◽  
Gyeong-Dong Kim ◽  
Chi-Ho Lee ◽  
Myung-Hwa Kang ◽  
Kwan-Sik Min
Keyword(s):  
Diagnostic Testing ◽  
Bovine Viral Diarrhoea Virus ◽  
Dairy Farms ◽  
Rapid Screening ◽  
Pcr Analysis ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Bulk Milk ◽  
Diarrhoea Virus ◽  
Targeted Testing

A total of 2,194 bulk-tank milks from dairy farms were investigated, and of which 842 dairy farms (55,263 cattle) were assessed to detect persistently infected (PI) individual cattle by performing ELISA. Reverse transcription (RT)-PCR and immunohistochemistry (IHC) were used to develop a rapid screening test for detecting Bovine viral diarrhoea virus (BVDV) in ear tissues. Testing for the bulk-milk tank from dairy farms showed that 2,007 (91.5%) out of a total of 2,194 farms were determined to be positive for BVDV, while only 187 bulk-milk tanks (8.5%) were negative. A total of 55,263 cattle were tested for BVDV, of which 669 (1.2%) cattle from 387 farms (46%) were identified as PI. The distribution of PI was 178 (65.4%) of 272 herds under 14 months. A 220 (80.9%) herds were found in less than 23 month ages. Four infected herds were identified as PI based on RT-PCR analysis. BVDV protein was shown to be localized within epidermal hair bulb cells. This approach of targeted testing of dairy herds using ELISA and RT-PCR for pre-diagnostic testing proves to markedly reduce BVDV-infected herds in Korean dairy farms and in Korean Native Cows.

A survey for bvdv antibodies in cattle farms in Slovakia and genetic typing of bvdv isolates from imported animals

2003 ◽  
Vol 51 (2) ◽  
pp. 229-236 ◽  
Author(s):  
Š. Vilček ◽  
Jana Mojžišová ◽  
Viera Bajová ◽  
Š. Paulík ◽  
L. Strojný ◽  
...  
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Computer Assisted ◽  
Serological Survey ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Serum Samples ◽  
Genetic Typing ◽  
Diarrhoea Virus ◽  
Pcr Products ◽  
Cattle Farms

A serological survey for bovine viral diarrhoea virus (BVDV) antibodies on a collection of 1295 serum samples obtained from 6-12 months old cattle originating from 45 farms in Slovakia was carried out. On 13 farms more than 90% of the examined animals were seropositive, on 14 farms 71-90% seroprevalence was observed, on 13 farms only 50-70% animals were found to be positive for BVDV antibodies, while the remaining 5 farms showed fewer than 50% seropositive animals. The average incidence of BVDV antibodies (around 70%) was similar as determined 30 years ago. Of 84 serum samples from seronegative animals originating from 14 farms in which 70-98% seropositivity was observed, six were positive in Ag-BVDV ELISA indicating persistently infected (PI) cattle. On a farm to which animals were imported from abroad, a BVD outbreak was observed. Of 110 animals tested, four were positive in Ag-ELISA indicating the presence of PI cattle on this farm. Genetic typing of two isolates from imported animals performed by RT-PCR (324/326 primers from 5´-UTR), sequencing of PCR products and computer-assisted phylogenetic analysis revealed that they belong to BVDV-1h group.

Storage of bovine viral diarrhoea virus samples on filter paper and detection of viral RNA by a RT-PCR method

2001 ◽  
Vol 92 (1) ◽  
pp. 19-22 ◽  
Author(s):  
Štefan Vilček ◽  
Ladislav Strojny ◽  
Branislav Ďurkovič ◽  
Wigbert Rossmanith ◽  
David Paton
Keyword(s):  
Filter Paper ◽  
Bovine Viral Diarrhoea Virus ◽  
Viral Rna ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Diarrhoea Virus ◽  
Pcr Method

Effects of bovine viral diarrhoea virus on the fertility of cows

2013 ◽  
Vol 61 (2) ◽  
pp. 281-289 ◽  
Author(s):  
Sibel Yavru ◽  
Mehmet Kale ◽  
Mehmet Gulay ◽  
Orhan Yapici ◽  
Oya Bulut ◽  
...  
Keyword(s):  
Bovine Viral Diarrhoea Virus ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Blood Samples ◽  
Frozen Semen ◽  
Diarrhoea Virus ◽  
Mucous Discharge ◽  
Service Conception ◽  
Negative Effect ◽  
Polymerase Chain

The aim of the present study was to determine the possible relationship between bovine viral diarrhoea (BVD) virus infection and the appearance of cervical mucous discharge (CMD) and the reproductive performance of cows in oestrus. For this purpose, CMD from 97 Holstein cows in oestrus was evaluated visually before artificial insemination (AI). Cows in oestrus were inseminated with frozen semen free from BVD virus (BVDV). Blood samples were tested by enzyme-linked immunoassay (ELISA) for antigen (Ag) and antibodies (Ab) of BVDV. The presence of the BVDV genome in cervical mucus samples was tested by reverse transcriptase-polymerase chain reaction (RT-PCR). The presence of BVDV Ab, Ag or genome was not associated with abnormal cervical mucous discharge (A-CMD). However, the presence of BVDV Ag (but not of the BVDV Ab) in blood samples was associated with a lower first service conception rate (FSCR; 27.8 vs. 70.9%; P < 0.01), indicating that BVDV viraemia at the time of AI has a negative effect on the fertility of cows.

Direct RT-PCR from serum enables fast and cost-effective phylogenetic analysis of bovine viral diarrhoea virus

2013 ◽  
Vol 190 (1-2) ◽  
pp. 1-3 ◽  
Author(s):  
Claudia Bachofen ◽  
Kim Willoughby ◽  
Ruth Zadoks ◽  
Paul Burr ◽  
Dominic Mellor ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Bovine Viral Diarrhoea Virus ◽  
Cost Effective ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Diarrhoea Virus

scholarly journals Genotyping of bovine viral diarrhoea virus isolates from the Czech Republic

2009 ◽  
Vol 54 (No. 9) ◽  
pp. 393-398 ◽  
Author(s):  
B. Robesova ◽  
K. Kovarcik ◽  
S. Vilcek
Keyword(s):  
Czech Republic ◽  
Bovine Viral Diarrhoea Virus ◽  
Computer Assisted ◽  
Bovine Viral Diarrhoea ◽  
Rt Pcr ◽  
Serum Samples ◽  
The Czech Republic ◽  
Genetic Typing ◽  
Diarrhoea Virus ◽  
Virus Isolates

This study was focused on the genetic typing of bovine viral diarrhoea virus (BVDV) isolates obtained from 41 serum samples of persistently infected cattle in the Czech Republic in the period of 2004 to 2007. For the differentiation of BVDV isolates, the 5’-UTR and Npro</sup> regions were selected. A 288-bp fragment from 5’-UTR and 428-bp fragment from Npro of the selected isolates were amplified by RT-PCR and subsequently sequenced and analysed by computer-assisted phylogenetic analysis. The isolates belong to BVDV-1 genotype and the following subtypes were identified: b (<i>n</i> = 16), d (<i>n</i> = 16), e (<i>n</i> = 2) and f (<i>n</i> = 7). In this collection of viral samples, no isolate belonged to BVDV-2 genotype.

Sign in / Sign up

Export Citation Format

Share Document