Reduction of non-specific reactions to the Brucella abortus serum agglutination test by the addition of EDTA

1985 ◽  
Vol 38 (3) ◽  
pp. 288-291 ◽  
Author(s):  
A.P. Macmillan ◽  
D.S. Cockrem
Keyword(s):  
Brucella Abortus ◽  
Agglutination Test ◽  
Specific Reactions ◽  
Serum Agglutination

scholarly journals The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests

1982 ◽  
Vol 88 (1) ◽  
pp. 11-19 ◽  
Author(s):  
R. J. Chappel ◽  
J. Hayes ◽  
B. A. Rogerson ◽  
L. J. Shenfield
Keyword(s):  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Serological Response ◽  
Serum Samples ◽  
Plate Test ◽  
The Rose ◽  
Serum Agglutination ◽  
Haemolysis Test

SummarySerum samples were obtained from 281 heifers vaccinated with Brucella abortus strain 19, and from 50 heifers that had received two injections of killed B. abortus strain 45/20 adjuvant (K45/20A) vaccine. The serological response measured by the brucellosis radioimmunoassay (RIA) was compared with responses measured by other tests.The serological responses of cattle during the first weeks after strain 19 vaccination were found to give little guide to the frequency of persistent reactions.In the case of strain 19, persistent reactions were considered to be those occurring 12 or more months after vaccination. In heifers vaccinated at the recommended age, small numbers of persistent reactions were given by the RIA (four in 374 sera), the complement fixation test using warm fixation (CFTW) (six in 383) and cold fixation (one in 185), the serum agglutination test (two in 222) and the indirect haemolysis test (IHLT) (two in 369). The Rose Bengal plate test gave 74 persistent reactions in 374 sera.Five of the 50 heifers gave particularly prolonged responses to K45/20A vaccine. In these animals the RIA and IHLT remained positive for longer than the CFTW.

scholarly journals Radioimmunoassay for antibodies against Brucella abortus: a new serological test for bovine brucellosis

1976 ◽  
Vol 77 (3) ◽  
pp. 369-376 ◽  
Author(s):  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught ◽  
M. J. Dalling ◽  
G. S. Allan
Keyword(s):  
Specific Antibody ◽  
Brucella Abortus ◽  
Bovine Serum ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Serological Test ◽  
Agglutination Test ◽  
Fixation Test ◽  
Bovine Brucellosis ◽  
Serum Agglutination

SUMMARYA radioimmunoassay (RIA) has been developed to measure antibodies against Brucella abortus in bovine serum and can be used in the diagnosis of bovine brucellosis. The RIA measures the amount of specific antibody of the IgG1 and IgG2 subclasses but is insensitive to 1gM, a characteristic which may make it more suitable than the complement fixation test (OFT) or the serum agglutination test for distinguishing infected animals from those which have been vaccinated with Br. abortus strain 19. The RIA is not subject to prozoning or ambiguous reactions, both of which interfere with the interpretation of the CFT.

scholarly journals Immune Responses and Protection against Experimental Brucella suis Biovar 1 Challenge in Nonvaccinated or B. abortus Strain RB51-Vaccinated Cattle

2010 ◽  
Vol 17 (12) ◽  
pp. 1891-1895 ◽  
Author(s):  
S. C. Olsen ◽  
S. G. Hennager
Keyword(s):  
Immune Responses ◽  
Experimental Infection ◽  
Brucella Abortus ◽  
Agglutination Test ◽  
Saline Control ◽  
Infected Cattle ◽  
Infection Rates ◽  
Treatment Groups ◽  
Brucella Suis ◽  
Fluorescence Polarization Assay

ABSTRACT Twenty Hereford heifers approximately 9 months of age were vaccinated with saline (control) or 2 × 1010 CFU of the Brucella abortus strain RB51 (RB51) vaccine. Immunologic responses after inoculation demonstrated significantly greater (P < 0.05) antibody and proliferative responses to RB51 antigens in cattle vaccinated with RB51 than in the controls. Pregnant cattle received a conjunctival challenge at approximately 6 months of gestation with 107 CFU of B. suis bv. 1 strains isolated from naturally infected cattle. The fluorescence polarization assay and the buffered acid plate agglutination test had the highest sensitivities in detecting B. suis-infected cattle between 2 and 12 weeks after experimental infection. Serologic responses and lymphocyte proliferative responses to B. suis antigens did not differ between control and RB51 vaccinees after experimental infection. No abortions occurred in cattle in either treatment group after challenge, although there appeared to be an increased incidence of retained placenta after parturition in both the control and the RB51 vaccination treatment groups. Our data suggest that the mammary gland is a preferred site for B. suis localization in cattle. Vaccination with RB51 did not reduce B. suis infection rates in maternal or fetal tissues. In conclusion, although B. suis is unlikely to cause abortions and fetal losses in cattle, our data suggest that RB51 vaccination will not protect cattle against B. suis infection after exposure.

COMPARISON OF EFFICIENCY OF THE RAPID WHOLE BLOOD AGGLUTINATION TEST WITH THE SERUM AGGLUTINATION TEST FOR PULLORUM DISEASE

1932 ◽  
Vol 6 (4) ◽  
pp. 381-386 ◽  
Author(s):  
Jacob Biely ◽  
William Roach
Keyword(s):  
Laboratory Test ◽  
Whole Blood ◽  
Low Cost ◽  
Agglutination Test ◽  
Practical Application ◽  
Serum Agglutination

The results obtained with the rapid whole blood agglutination test for pullorum disease, applied in the field, agree closely with the results secured with the rapid serum agglutination test, applied in the laboratory.The accuracy of the diagnosis was found to depend upon the training and experience of the technician. When the whole blood agglutination test was applied by inexperienced persons, the results obtained differed from the laboratory test by 12% as compared with a difference of 1.3% when the whole blood agglutination test was applied by an experienced technician.The rapid whole blood agglutination test was found to lend itself very readily to practical application in the field. The extremely low cost makes feasible the application and repetition of the test on a large scale.Since it is known from previous work that one agglutination test will not eliminate all carriers of pullorum disease, the rapid whole blood agglutination test should be applied several times a year until at least two successive negative tests are obtained on each bird of the flock.

A COMPARISON OF THE ROUTINE RAPID WHOLE BLOOD (STAINED ANTIGEN) AND THE ROUTINE RAPID SERUM AGGLUTINATION TESTS FOR PULLORUM DISEASE

1934 ◽  
Vol 10 (6) ◽  
pp. 798-806
Author(s):  
Jacob Biely ◽  
W. Roach
Keyword(s):  
Whole Blood ◽  
Agglutination Test ◽  
Test Laboratory ◽  
Post Mortem ◽  
Post Mortem Examination ◽  
Experiment Station ◽  
Commercial Laboratory ◽  
Serum Agglutination

Data are presented on 4,429 birds, comprising eight flocks, which were tested for pullorum disease by the whole blood agglutination test and the rapid serum agglutination test (commercial laboratory). The diagnoses agreed in the cases of 4,046 birds (97.24%) and disagreed in the cases of 122 birds (2.75%).Of the 122 birds, 43 were diagnosed as positive by the whole blood agglutination test and as negative by the rapid serum agglutination test, while 79 were diagnosed as positive by the rapid serum agglutination test and as negative by the whole blood agglutination test.Of the 122 birds, 102 were retested by the whole blood, rapid serum (Laboratory 1), and rapid serum agglutination test (Laboratory 2, (Experiment Station Laboratory)).There was a closer agreement between the diagnoses made on the basis of the whole blood and rapid serum tests (Laboratory 2) than between those made on the basis of the rapid serum (Laboratory 1) and rapid serum (Laboratory 2) tests (71.56% and 62.37% respectively).A detailed study of the retests and post-mortem examination of the 102 birds is presented.

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

scholarly journals Bovine brucellosis in slaughterhouses controlled by Federal and Municipal Inspection Services in the state of Maranhão, Brazil

2019 ◽  
Vol 86 ◽  
Author(s):  
Anna Karoline Amaral Sousa ◽  
Bruno Raphael Ribeiro Guimarães ◽  
Priscila Alencar Beserra ◽  
Danilo Cutrim Bezerra ◽  
Ferdinan de Almeida Melo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Brucella Abortus ◽  
Bovine Brucellosis ◽  
Blood Samples ◽  
Male And Female ◽  
Specific Antibodies ◽  
Laboratory Results ◽  
Inspection Service ◽  
Serum Agglutination ◽  
Do So

ABSTRACT: The aims of the current study were to investigate the prevalence of bovine brucellosis, to correlate laboratory results of serum reactive samples to the serology of animals presenting serous pouches, and to identify possible risk factors for the development of the disease. In order to do so, 1,265 bovine blood samples were collected from male and female animals bred in different counties in Maranhão state, Brazil, and in other regions of the country. The animals were slaughtered in two slaughterhouses controlled by the Federal Inspection Service (FIS), and in two others controlled by the Municipal Inspection Service (MIS), of regions such as Açailândia and Imperatriz. Samples were tested for specific antibodies by using buffered acidified antigen (BAA) and 2-mercaptoethanol in combination with slow serum agglutination (2-ME + SSA). Additionally, an epidemiological questionnaire was applied to 100 owners, who provided animals to the slaughterhouses, to enable investigating the risk factors for bovine brucellosis. Fifteen serous pouches of animals presenting this lesion were also collected at slaughter time. Thirty-nine out of the analyzed samples were reacted to BAA, whereas 15 reacted to the 2-ME + SSA test; only one male was reagent and it resulted in 1.19% prevalence. One out of the 15 collected serous pouches reacted to the 2-ME + SSA test. The risk factors identified in this study were: the incidence of miscarriages, the sale of animals without previous examination, and the failure in testing the animals before introducing them in herds and before slaughter. It was possible to conclude that the animals slaughtered in the herein evaluated slaughterhouses were infected with Brucella abortus, as well as that this infection prevailed in females.

scholarly journals Protective Effect of the Nramp1 BB Genotype against Brucella abortus in the Water Buffalo (Bubalus bubalis)

2006 ◽  
Vol 75 (2) ◽  
pp. 988-996 ◽  
Author(s):  
Rosanna Capparelli ◽  
Flora Alfano ◽  
Maria Grazia Amoroso ◽  
Giorgia Borriello ◽  
Domenico Fenizia ◽  
...  
Keyword(s):  
Brucella Abortus ◽  
Untranslated Region ◽  
Complement Fixation Test ◽  
Water Buffalo ◽  
Complement Fixation ◽  
Bubalus Bubalis ◽  
Lower Number ◽  
Agglutination Test ◽  
Intracellular Bacteria ◽  
Antibacterial Protein

ABSTRACT We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3′ untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.

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