Ultrasensitive determination of trans-β-carotene in rat and beef livers by means of high-performance liquid chromatography coupled with thermal lens detection

Talanta ◽  
2000 ◽  
Vol 53 (1) ◽  
pp. 103-113 ◽  
Author(s):  
S Luterotti
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thermal Lens ◽  
High Performance ◽  
Β Carotene

Rapid method for determination of β-carotene in foods using ultra high performance liquid chromatography

2010 ◽  
Vol 19 (5) ◽  
pp. 1199-1204 ◽  
Author(s):  
Jaeho Ha ◽  
You-Shin Shim ◽  
Hye-Young Seo ◽  
Hyun-Jin Nam ◽  
Masahito Ito ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Rapid Method ◽  
High Performance ◽  
Β Carotene

Determination of Lutein and β-Carotene in Infant Formula and Adult Nutritionals by Ultra-High-Performance Liquid Chromatography: Single-Laboratory Validation, First Action 2016.13

2017 ◽  
Vol 100 (3) ◽  
pp. 768-781
Author(s):  
Gregory L Hostetler
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Infant Formula ◽  
High Performance ◽  
Hplc Method ◽  
Method Performance ◽  
Performance Requirements ◽  
Single Laboratory ◽  
Β Carotene

Abstract An ultra-HPLC method for the determination of lutein and β-carotene in infant formula and adult nutritionals wasvalidated using both unfortified and fortified samples provided by the AOAC Stakeholder Panel on Infant Formula and AdultNutritionals (SPIFAN). All experiments showed separation of all-trans-lutein and β-carotene from their major cis isomers, apocarotenal, α-carotene, lycopene, and zeaxanthin. Samples spiked with all-trans-lutein and β-carotene showed no isomerization during sample preparation. Linearity of the calibration solutions correlated to approximately 0.8–45 μg/100 g (reconstituted basis) for samples prepared for the lowest sample concentrations. With dilutions specified in the method, the range can be extended to approximately 2250 μg/100 g. The LOD for both lutein and β-carotene was 0.08 μg/100 g, and the LOQ for both was 0.27μg/100 g. For all measurements in the range of 1–100 μg/100 g, repeatability RSD was ≤5.8% forlutein and ≤5.1% for β-carotene. For measurements >100 μg/100 g, repeatability RSD was ≤1.1% for lutein and ≤1.7% for β-carotene. Accuracywas determined by recovery from spiked samples and ranged from 92.3 to 105.5% for lutein and from 100.1 to 107.5% for β-carotene. The data provided show that the method meets the criteria specified in the Standard Method Performance Requirements for carotenoids (SMPR 2014.014).

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