Age-dependent changes in the susceptibility to apoptosis of peripheral blood CD4+ and CD8+ T lymphocytes with virgin or memory phenotype

2003 ◽  
Vol 124 (4) ◽  
pp. 409-418 ◽  
Author(s):  
Stefano Salvioli ◽  
Miriam Capri ◽  
Elena Scarcella ◽  
Sandro Mangherini ◽  
Irma Faranca ◽  
...  
1998 ◽  
Vol 22 (2) ◽  
pp. 239-248 ◽  
Author(s):  
Ki-Hoan Nam ◽  
Hirofumi Akari ◽  
Keiji Terao ◽  
Hiromi Ohto ◽  
Shinichi Itagaki ◽  
...  

2002 ◽  
Vol 98 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Franck Housseau ◽  
Daniel A. Langer ◽  
Samuel D. Oberholtzer ◽  
Anitha Moorthy ◽  
Hyam I. Levitsky ◽  
...  

2005 ◽  
Vol 58 (2) ◽  
pp. 252-258 ◽  
Author(s):  
Richard Shimonkevitz ◽  
JoBeth Northrop ◽  
Lisbeth Harris ◽  
Michael Craun ◽  
David Bar-Or

2007 ◽  
Vol 139 (5) ◽  
pp. 780-790 ◽  
Author(s):  
Paolo Ghia ◽  
Giuseppina Prato ◽  
Stefania Stella ◽  
Cristina Scielzo ◽  
Massimo Geuna ◽  
...  

Gerontology ◽  
2009 ◽  
Vol 55 (3) ◽  
pp. 314-321 ◽  
Author(s):  
Mauro Provinciali ◽  
Raffaella Moresi ◽  
Alessia Donnini ◽  
Rosa Maria Lisa

2021 ◽  
Vol 12 ◽  
Author(s):  
Matilda J. Moström ◽  
Elizabeth A. Scheef ◽  
Lesli M. Sprehe ◽  
Dawn Szeltner ◽  
Dollnovan Tran ◽  
...  

The maternal decidua is an immunologically complex environment that balances maintenance of immune tolerance to fetal paternal antigens with protection of the fetus against vertical transmission of maternal pathogens. To better understand host immune determinants of congenital infection at the maternal-fetal tissue interface, we performed a comparative analysis of innate and adaptive immune cell subsets in the peripheral blood and decidua of healthy rhesus macaque pregnancies across all trimesters of gestation and determined changes after Zika virus (ZIKV) infection. Using one 28-color and one 18-color polychromatic flow cytometry panel we simultaneously analyzed the frequency, phenotype, activation status and trafficking properties of αβ T, γδ T, iNKT, regulatory T (Treg), NK cells, B lymphocytes, monocytes, macrophages, and dendritic cells (DC). Decidual leukocytes showed a striking enrichment of activated effector memory and tissue-resident memory CD4+ and CD8+ T lymphocytes, CD4+ Tregs, CD56+ NK cells, CD14+CD16+ monocytes, CD206+ tissue-resident macrophages, and a paucity of B lymphocytes when compared to peripheral blood. t-distributed stochastic neighbor embedding (tSNE) revealed unique populations of decidual NK, T, DC and monocyte/macrophage subsets. Principal component analysis showed distinct spatial localization of decidual and circulating leukocytes contributed by NK and CD8+ T lymphocytes, and separation of decidua based on gestational age contributed by memory CD4+ and CD8+ T lymphocytes. Decidua from 10 ZIKV-infected dams obtained 16-56 days post infection at third (n=9) or second (n=1) trimester showed a significant reduction in frequency of activated, CXCR3+, and/or Granzyme B+ memory CD4+ and CD8+ T lymphocytes and γδ T compared to normal decidua. These data suggest that ZIKV induces local immunosuppression with reduced immune recruitment and impaired cytotoxicity. Our study adds to the immune characterization of the maternal-fetal interface in a translational nonhuman primate model of congenital infection and provides novel insight in to putative mechanisms of vertical transmission.


2018 ◽  
Vol 45 (1) ◽  
pp. 378-388 ◽  
Author(s):  
Chen Jing ◽  
Zheng Dongming ◽  
Cui Hong ◽  
Na Quan ◽  
Liu Sishi ◽  
...  

Background/Aims: To detect the expression of the TRPC3 channel protein in the tissues of women experiencing preterm labor and investigate its interaction with T lymphocytes, providing a theoretical basis for the clinical prevention of threatened preterm labor and the development of drug-targeted therapy. Methods: Forty-seven women experiencing preterm labor and 47 women experiencing normal full-term labor were included in this study. All included women underwent delivery via cesarean section; uterine samples were obtained at delivery. The expression of TRPC3 in uterine tissue was detected by immunohistochemistry, real-time quantitative reverse transcription-PCR, and western blot assay. Activation of T lymphocytes in peripheral blood and uterine tissue were detected by flow cytometry. A TRPC3-/- mouse model of inflammation-induced preterm labor was established; expression of TRPC3, Cav3.1, and Cav3.2 were analyzed in mouse uterine tissue. Activation of T lymphocytes in female mouse and human peripheral blood samples was determined using flow cytometry. Results: In women experiencing preterm labor, expression of TRPC3 and the Cav3.1 and Cav3.2 proteins was significantly increased; in addition, the percentage of CD3+, CD4+, and CD8+ T cells in peripheral blood was significantly decreased. TRPC3 knockout significantly delayed the occurrence of preterm labor in mice. The muscle tension of ex vivo uterine strips was lower, Cav3.1 and Cav3.2 protein expression was lower, and the percentage of CD8+ T lymphocytes was significantly increased in wild-type mice subjected to an inflammation-induced preterm labor than in wild-type mice experiencing normal full-term labor. Conclusion: TRPC3 is closely related to the initiation of labor. TRPC3 relies on Cav3.1 and Cav3.2 proteins to inhibit inflammation-induced preterm labor by inhibiting the activation of T cells, in particular CD8+ T lymphocytes.


1999 ◽  
Vol 91 (3) ◽  
pp. 321-329 ◽  
Author(s):  
Hirofumi Akari ◽  
Ki-Hoan Nam ◽  
Kazuyasu Mori ◽  
Isao Otani ◽  
Hiroaki Shibata ◽  
...  

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