Specific IgE/IgG determinations to diphtheria-tetanus vaccine: Studies on the cross-reactivity

2002 ◽  
Vol 109 (1) ◽  
pp. S144-S145
Author(s):  
F Martin ◽  
MJ Pereira ◽  
S Posadas ◽  
E Sanchez ◽  
M Blanca ◽  
...  
Keyword(s):  
Specific Ige ◽  
Cross Reactivity ◽  
Tetanus Vaccine ◽  
The Cross

Cross-Reactivity between IgE-Binding Proteins from Anisakis Simplex and Dermatophagoides Pteronyssinus

2005 ◽  
Vol 18 (4) ◽  
pp. 671-675 ◽  
Author(s):  
R. Bernardini ◽  
G. Mistrello ◽  
E. Novembre ◽  
D. Roncarolo ◽  
S. Zanotta ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Binding Proteins ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
Cross Reactivity ◽  
Anisakis Simplex ◽  
Cross Reaction ◽  
Ige Binding ◽  
Sds Page ◽  
The Cross

An association was found between Anisakis simplex (As) and Dermatophagoides pteronyssinus (Dp) sensitization. One recent study shows a cross-reactivity between As and Dp and tropomyosin (tr) is suspected as being one of the proteins responsible of this cross-reaction. The aim of our study was: 1) to confirm the cross-reactivity between Dp and As; 2) to determine the importance of tr in this cross reaction. SDS-PAGE analysis of Dp and As (metabolic and somatic) extracts was carried out. Then an IgE immunoblotting test using serum from a patient who had specific IgE only to Dp and As and immunoblotting inhibition experiments using Dp extract and tr as inhibitors were performed. We found that patient's serum reacted: 1) against larval As antigens with a molecular weight (mw) of 25 kilodalton (kD) and a mw > 100 kD, 2) against various metabolic As antigens with a mw > 100 kD, a mw ranging approximately from 35 to 50 kD, and a mw around 20 kD, and 3) against Dp proteins with mw between 35 and 55 kD. Preincubation of patient's serum with Dp extract caused the disappearance of reactivity against antigens with a mw > 100 kD in both larval and metabolic As extracts and against proteins with mw ranging approximately from 35 to 50 kD in the metabolic As extract. Preincubation of patient's serum with As extract caused the disappearance of reactivity against antigens with mw between 35 and 55 kD in the Dp extract. Pre-incubation of patient's serum with tr did not induce any change in the immunoblotting profile. The results show that 1) cross-reactive components between Dp and As are some proteins with a mw ranging approximately from 35 to 50 kD and with a mw > 100 kD, and 2) tr is not involved in cross-reactivity between As and Dp.

scholarly journals Allergenic Characterization of Tropomyosin from the Dusky Brown Cockroach, Periplaneta fuliginosa

2004 ◽  
Vol 11 (4) ◽  
pp. 680-685 ◽  
Author(s):  
Kyoung Yong Jeong ◽  
Heeyu Hwang ◽  
Jongweon Lee ◽  
In-Yong Lee ◽  
Dong Soo Kim ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Periplaneta Americana ◽  
Immunoglobulin E ◽  
Expression System ◽  
Specific Ige ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inhibitor Concentration ◽  
The Cross ◽  
Elisa Inhibition

ABSTRACTHousehold arthropods are one of the most common causes of allergic diseases. Four species of cockroaches are found to reside in Korean homes, but published work deals almost exclusively with the German and American cockroaches. This study was undertaken to investigate the cross-reactive allergenic components of the dusky brown cockroach,Periplaneta fuliginosa. Enzyme-linked immunosorbent assay (ELISA) inhibition and immunoblot analyses for the dusky brown cockroach were performed withBlattella germanicaandDermatophagoides farinaeallergic sera. cDNA encoding tropomyosin, which is a well known cross-reactive pan-allergen, was cloned by reverse transcriptase PCR, and recombinant protein was produced by using a pET-28b expression system. Native tropomyosin was purified by ammonium sulfate fractionation and electroelution. The immunoglobulin E (IgE) reactivities of native and recombinant tropomyosins were compared by an ELISA inhibition study. All 30 sera tested showedP. fuliginosa-specific IgE, and the IgE-binding reactivity of theP. fuliginosaextract was inhibited as much as 79.4% by aB. germanicaextract and as much as 63.3% by aD. farinaeextract. The deduced amino acid sequence of cloned cDNA was identical with that ofPeriplaneta americanatropomyosin (98.5% nucleotide sequence identity). Seven of 26 (26.9%) allergic sera had IgE specific for recombinant protein, and the maximum inhibition ofP. fuliginosa-specific IgE achieved with recombinant tropomyosin was 37.7% at an inhibitor concentration of 10 μg/ml. Native tropomyosin inhibited the binding of IgE to theP. fuliginosa,B. germanica, andD. farinaeextracts by 65.0, 51.8, and 39% at an inhibitor concentration of 1 μg/ml.P. fuliginosaappears to possess allergens that are highly cross-reactive with allergens ofB. germanicaandD. farinae. Tropomyosin was found to be a major allergenic component accounting for the cross-reactivity between cockroaches and dust mites.

Analysis of Cross-Reactivity and Allergic Symptoms of 19 Allergens: Results from NHANES 2005–2006

2021 ◽  
pp. 1-10
Author(s):  
Zhi-Ling Zhu ◽  
Ying-Xing Wu ◽  
Zhu-Ping Zhang ◽  
Song Li
Keyword(s):  
Respiratory Health ◽  
Specific Ige ◽  
Cross Reactivity ◽  
The Body ◽  
Cross Sectional ◽  
Allergic Symptoms ◽  
Allergen Sources ◽  
The Usa ◽  
The Cross ◽  
Using Data

<b><i>Introduction:</i></b> We explored the cross-reactivity among 19 common allergen sources and evaluated the influence of serum IgE concentrations and the number of sensitized allergens on the incidence of allergic symptoms. <b><i>Methods:</i></b> We conducted this cross-sectional analysis using data from the National Health and Nutrition Examination Survey (NHANES) 2005–2006 which is a program of studies designed to assess the health and nutritional status of adults and children in the USA. After excluding participants with missing data from the allergen IgE test, allergy questionnaire, and respiratory health questionnaire, a total of 7,224 participants aged 6 years and older were included, as children younger than 6 years old did not complete all 19 allergen-specific IgE tests. Spearman correlation analysis was used to analyze the cross-reactivity between allergen sources. An independent sample Kruskal-Wallis test was performed to investigate the relationship between the serum-specific IgE levels of 19 allergens and the incidence of allergic symptoms. <b><i>Results:</i></b> The cross-reactivity between <i>D. farinae</i> and <i>D. pteronyssinus</i> was the strongest (ρ = 0.88), and cross-reactivity of cross-species was universal. With the increase in serum-specific IgE levels of <i>D. farinae</i>, <i>D. pteronyssinus</i>, oak, and birch, the incidence of sneezing increased (<i>p</i> &#x3c; 0.05). With the increase in serum-specific IgE levels of cats, dogs, peanuts, <i>Aspergillus</i>, and <i>Alternaria</i>, the incidence of wheezing increased (<i>p</i> &#x3c; 0.05). The incidence of rash was positively correlated with serum-specific IgE levels of <i>D. farinae</i>, <i>D. pteronyssinus</i>, shrimp, and peanut (<i>p</i> &#x3c; 0.05). The incidence of wheezing continued to increase with an increase in sensitized allergens. When participants were sensitized to &#x3c;10 allergens, the incidence of sneezing continued to increase as the number of sensitized allergens increased, whereas the incidence of rash did not have a clear association with the number of sensitized allergens. <b><i>Conclusion:</i></b> Species that are biologically close are more likely to have antigen cross-reactivity, while cross-reactivity among different species is common. Different allergens tend to cause different allergic symptoms. Different allergic sites in the body have inconsistent responses to the number of sensitized allergens.

Food antigen-specific IgE in dogs with suspected food hypersensitivity

2020 ◽  
Vol 48 (06) ◽  
pp. 395-402
Author(s):  
Sandra A. Baumann ◽  
Cornelius Fritz ◽  
Ralf S. Mueller
Keyword(s):  
Clinical Relevance ◽  
Specific Ige ◽  
Elimination Diet ◽  
Cross Reactivity ◽  
Food Hypersensitivity ◽  
Strong Correlations ◽  
Odds Ratios ◽  
Cross Reactions ◽  
Ige Reactivity ◽  
Food Specific Ige

Abstract Objective Knowledge of cross-reactions in food-sensitive dogs will influence the choice of elimination diets and the long-term management of those patients. The objective of this study was to evaluate food allergen-specific IgE tests of suspected allergic dogs for concurrent positive reactions as possible evidence for cross reactions between allergens. Material and methods Results of serum IgE tests from 760 suspected allergic dogs submitted to 2 laboratories were evaluated statistically. After the tested allergens were grouped by their phylogenetic relationship, odds ratios as well as a sensitivity analysis of the odds ratios were performed to evaluate if concurrent positive IgE results to 2 allergens occurred more often than expected. Results Within related allergen pairs 27% (laboratory 1) and 72% (laboratory 2) of the pairs could be considered as associated. For the unrelated allergen pairs only 6.8% and 10.6% of the analyzed pairs were considered associated respectively. Strong correlations were shown in the group of ruminant allergens, especially beef and lamb, and grain allergens. High rates of concurrent reactions were also detected in the poultry group, especially between chicken and duck, as well as between pork and ruminant allergens, and soy and grain allergens. Conclusion As our results showed not only correlations within related but also between non-related allergens, the possible relevance of carbohydrate moieties as well as panallergens for canine hypersensitivities warrants further study. Further investigations are necessary to distinguish co-sensitization from cross-reactions and determine the clinical relevance of food-specific IgE reactivity. Clinical relevance Due to possible cross reactivity related allergens, especially beef and lamb as well as grain allergens, should not be used for an elimination diet to avoid false results.

scholarly journals Peanut-specific IgE antibodies in asymptomatic Ghanaian children possibly caused by carbohydrate determinant cross-reactivity

2013 ◽  
Vol 132 (3) ◽  
pp. 639-647 ◽  
Author(s):  
Abena S. Amoah ◽  
Benedicta B. Obeng ◽  
Irene A. Larbi ◽  
Serge A. Versteeg ◽  
Yvonne Aryeetey ◽  
...  
Keyword(s):  
Specific Ige ◽  
Cross Reactivity ◽  
Ige Antibodies ◽  
Specific Ige Antibodies

Cross-reactivity of rapid immunochemical methods for mycotoxins detection towards metabolites and masked mycotoxins: the current state of knowledge

2014 ◽  
Vol 7 (4) ◽  
pp. 449-464 ◽  
Author(s):  
M. Zachariasova ◽  
P. Cuhra ◽  
J. Hajslova
Keyword(s):  
Rapid Test ◽  
Cross Reactivity ◽  
Rapid Screening ◽  
Proficiency Tests ◽  
Assigned Value ◽  
Screening Practice ◽  
Current State ◽  
Immunochemical Methods ◽  
The Cross ◽  
Lateral Flow Test

The cross-reactivity of antibodies employed within immunochemistry-based analytical methods may lead to overestimation of the results. Under certain conditions, specifically when controlling mycotoxin maximum limits serious problems can be encountered. Not only the structurally related mycotoxins, such as their masked (conjugated) forms, but also the unidentified matrix components are responsible for concentration overestimation of respective target analytes. The cross-reactivity phenomenon may also pose a risk of miss-interpretation of the proficiency tests results, when the assigned value becomes influenced by over-estimated results reported by users of immunochemical tests. In this paper, the current state of the knowledge on trueness problems associated with the rapid screening immunochemical methods have been reviewed. Special attention is focused on discussion of cross-reactivity in the ELISA tests, because this rapid test dominates the routine screening practice. However, the cross-reactions reported in lateral flow test strips, fluorescence polarisation immunoassay, or immunosensors have also been addressed.

Immunity to tapeworms: intraspecific cross-protective interactions betweenHymenolepis citelli, H. diminutaandH. microstomain mice

Parasitology ◽  
1986 ◽  
Vol 92 (3) ◽  
pp. 665-674 ◽  
Author(s):  
Sidi T. O. Alghali ◽  
R. K. Grencis
Keyword(s):  
Primary Infection ◽  
Cross Reactivity ◽  
Significant Protection ◽  
Cyst Infection ◽  
Single Host ◽  
Concurrent Infections ◽  
Rejection Mechanism ◽  
The Cross

SUMMARYInteractions between tapeworm species in a single host offer intriguing opportunities for immunological studies that attempt to identify the mechanism(s) underlying protection against cestode infections. Mice that are immunized againstHymenolepis citelliinfections were shown to be refractory to subsequentH. diminutachallenge infections. The reciprocity of the response was also demonstrated, although the protection recorded forH. diminutawhen mice are sensitized withH. citelliis weaker than that observed when mice are primed withH. diminutaagainstH. citellichallenge.H. citelliwas also shown to be expelled simultaneously during the rejection phase ofH. diminutain concurrent infections, indicating the susceptibility of the former tapeworm to the rejection mechanism initiated by the latter.H. microstomaimmunized mice were shown to be strongly protected against heterologousH. citellichallenge. However, mice primed againstH. citelliwere not as strongly protected againstH. microstomachallenge infections: a statistically significant protection was obtained only after a 12-cysticercoidH. citelliprimary infection, although a 6-cyst infection did stunt the growth ofH. microstomachallenge worms. It is presently suggested that the cross-protective responses observed in the study betweenH. citelli, H. diminutaandH. microstomamay have emanated from a specific immunological cross-reactivity due to the sharing of similar immunogens.

scholarly journals Ceftaroline Desensitization Procedure in a Pregnant Patient With Multiple Drug Allergies

2015 ◽  
Vol 2 (1) ◽  
Author(s):  
James L. Kuhlen ◽  
Kimberly G. Blumenthal ◽  
Caroline L. Sokol ◽  
Diana S. Balekian ◽  
Ana A. Weil ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Skin Testing ◽  
Pregnant Patient ◽  
Cross Reactivity ◽  
Multiple Drug ◽  
Methicillin Resistant ◽  
The Cross ◽  
Staphylococcus Aureus Pneumonia

Abstract Validated skin testing is lacking for many drugs, including ceftaroline. The cross-reactivity between ceftaroline and other β-lactam antibiotics is unknown. We report a case of a pregnant patient with cystic fibrosis and multiple drug allergies who required ceftaroline for methicillin-resistant Staphylococcus aureus pneumonia and underwent an uncomplicated empiric desensitization procedure.

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