Ultrasonic morphology of the corpora lutea and central luteal cavities during selection of recipients for embryo transfer

1998 ◽  
Vol 49 (1) ◽  
pp. 243
Author(s):  
A. Garcia ◽  
M. Salaheddine
Keyword(s):  
Embryo Transfer ◽  
Corpora Lutea ◽  
Selection Of

Embryo transfer in the dromedary camel (Camelus dromedarius) using non-ovulated and ovulated, asynchronous progesterone-treated recipients

2011 ◽  
Vol 23 (3) ◽  
pp. 438 ◽  
Author(s):  
J. A. Skidmore ◽  
M. Billah
Keyword(s):  
Embryo Transfer ◽  
Chorionic Gonadotrophin ◽  
Camelus Dromedarius ◽  
Dromedary Camel ◽  
Corpora Lutea ◽  
Progesterone Treatment ◽  
Daily Dose ◽  
Exogenous Progesterone ◽  
To Receive

The aim of the present study was to investigate the use of exogenous progesterone and equine chorionic gonadotrophin (eCG) in non-ovulated and ovulated, asynchronous dromedary camel recipients being prepared for an embryo transfer programme. The uteri of 12 mated donor camels were flushed non-surgically 7 days after ovulation and 42 embryos were recovered. In Experiment 1, 16 embryos were transferred non-surgically to recipients on Day 3 or 4 after ovulation (ov+3 and ov+4, respectively). Each recipient received a daily dose of 75 mg, i.m., progesterone-in-oil from 2 days before embryo transfer until 6 days after ovulation. Thereafter, the progesterone dose was reduced to 50 mg on Day 7 and finally to 25 mg day–1 on Days 8 and 9. Nine of 16 recipients (56%; ov+3, n = 4; ov+4, n = 5) became pregnant compared with none of eight non-progesterone treated controls, into which embryos were transferred on Day 4 after ovulation. In Experiment 2, 18 non-ovulated recipients received 75 mg, i.m., progesterone-in-oil daily from 3 days before until 12 days after non-surgical transfer of a Day 7 blastocyst, at which time pregnancy was diagnosed by ultrasonography. All pregnant recipients continued to receive 75 mg progesterone-in-oil daily for a further 6 days, when each camel received 2000 IU, i.m., eCG. Progesterone treatment was then reduced to 50 mg day–1 and, when a follicle(s) ≥1.3 cm in diameter were present in the ovaries, each animal received 20 μg buserelin to induce ovulation. Once the corpora lutea had developed, progesterone treatment was reduced to 25 mg day–1 for a final 3 days. Fourteen of 18 recipients (78%) became pregnant and seven of these (50%) remained pregnant after eCG treatment. Of the seven pregnancies that were lost, two were lost before eCG treatment, two did not respond to eCG treatment and three responded to eCG treatment and ovulated, but lost their pregnancies 6–8 days after the last progesterone injection.

scholarly journals Studies on Application of Ultrasound for Selection of Carcass Traits on Donor Cow for Embryo Transfer in Japanese Black Cattle

1995 ◽  
Vol 66 (3) ◽  
pp. 247-252
Author(s):  
Tomoyuki YAKUSHIJI ◽  
Hiroshi HARADA ◽  
Nobuhiro KITA ◽  
P. G. DURAN
Keyword(s):  
Embryo Transfer ◽  
Carcass Traits ◽  
Japanese Black Cattle ◽  
Selection Of

scholarly journals Comparison of long-term selection responses of breeding policy in dairy herds

2011 ◽  
Vol 50 (No. 10) ◽  
pp. 439-449
Author(s):  
P. Šafus ◽  
J. Přibyl
Keyword(s):  
Embryo Transfer ◽  
Genetic Gain ◽  
Negative Selection ◽  
Milk Proteins ◽  
Breeding Value ◽  
Dairy Herds ◽  
Selection Intensity ◽  
Term Selection ◽  
Single Use ◽  
Selection Of

Genetic and economic responses to genetic gain were evaluated for these breeding arrangements: single use of bulls under testing; single and repeated use of proved bulls for two years and for three years (in combination with selection intensity 1%, 5% or 10% of the best bulls for the use of proved bulls); negative selection of cows in the herd &ndash; 10%, 20% or 30% of animals are discarded from reproduction and the animals are left in the herd and used for breeding by beef bulls; negative selection of first-calvers in the herd &ndash; 10%, 20% or 25% of animals are discarded for slaughter; discarding of heifers and purchase of animals with higher breeding value, and embryo transfer &ndash; a group of selected recipients accounts for 10%, 20% or 30% of the cows with lover breeding value in the dairy herds. Simulations for single use of breeding arrangements and their consequences over a fifty-year period were carried out for the above models using the gene flow method. Only minimum changes will occur since the 25<sup>th</sup> year of observation. A comparison of the particular models showed the highest gain of proved bulls selected from 1% of the best bulls whose cumulative genetic gain was 41.558 kg of milk proteins on average per cow for the whole observed period. Single use of bulls under testing in the herd resulted in the second highest cumulative genetic gain. Use of proved bulls selected with lower selection intensity (5% or 10%) had worse results. Culling of cows and discarding of first-calvers for slaughter led to lower genetic and economic contribution. The gain of embryo transfer was also lower; moreover, it is not economically advantageous for its very high costs, so it should not be used in production herds generally. High genetic and economic gain was recorded for replacement of all heifers by animals with higher breeding value from other populations. &nbsp;

scholarly journals Studies on factors affecting superovulation and embryo transfer in Hungarian Merino ewes

2001 ◽  
Vol 49 (4) ◽  
pp. 431-441 ◽  
Author(s):  
S. Cseh ◽  
L. Solti
Keyword(s):  
Developmental Stage ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Ovarian Stimulation ◽  
Corpora Lutea ◽  
Treatment Protocols ◽  
Factors Affecting ◽  
Factors Influencing ◽  
Significant Difference ◽  
One Year

The objectives of this study were (a) to assess the ovulatory response and embryo production of Hungarian Merino ewes after superovulation, (b) to investigate the factors influencing the efficiency of embryo transfer (ET) in Hungarian Merino ewes, (c) to compare the results of two ovarian stimulation protocols (PMSG and PMSG+FSH treatment) in Hungarian Merino ewes, and (d) to study how superovulation, laparoscopic insemination and surgical embryo retrieval (ER) affect the subsequent reproduction of Hungarian Merino donor females after an ET programme. There was no significant difference between the ovarian stimulation protocols in the ratio of donor ewes responding to superovulation nor in the average number of corpora lutea. However, the number of transferable embryos recovered per donor ewe was higher in the PMSG+FSH group. The proportion of transferable embryos, unfertilised oocytes and degenerated embryos did not differ between the treatment protocols. The total pregnancy rate was 53.4% (179/335). Neither the developmental stage of the embryo nor the number of transferred embryos affect the implantation of embryos. However, the increased number of transferred embryos positively influenced the pregnancy rate. No difference was found in the pregnancy rate between synchronised and nonsynchronised groups of recipients. Thirty-six out of 45 donor ewes (80%) became pregnant within one year after the ET programme, indicating that ovarian stimulation and surgical ER did not affect adversely their reproduction.

SELECTION OF SIRES ORIGINATING FROM A NUCLEUS BREEDING UNIT FOR USE IN A COMMERCIAL DAIRY POPULATION

1989 ◽  
Vol 69 (3) ◽  
pp. 595-604 ◽  
Author(s):  
GEORG TEEPKER ◽  
DAVID S. KELLER
Keyword(s):  
Embryo Transfer ◽  
Pedigree Information ◽  
Sources Of Information ◽  
Production Environment ◽  
Genetic Progress ◽  
Genotype By Environment Interactions ◽  
Genotype By Environment ◽  
Commercial Population ◽  
Combined Selection ◽  
Selection Of

Bulls that originate from breeding schemes with extensive use of multiple ovulation and embryo transfer (MOET) can be selected on pedigree information, the performance of their contemporary female full- and half-sibs or based on a progeny test. Combined selection across groups of bulls with different sources of information is shown to be superior to selection of progeny tested bulls alone. The magnitude of the superiority for a given selection rate is determined by the differences in genetic merit between the groups and ranges from 5 to 65%. Depending on the annual genetic progress the majority of the bulls are selected either on pedigree or progeny information. The proportion of sib-tested bulls ranges from 9 to 23%. When breeding activities are concentrated in nucleus herds, possible genotype by environment interactions between performance in the test herd and in the commercial population have to be considered. Genotype by environment interactions increase the proportion of progeny tested sires that will be selected. However, the proportion of progeny tested sires used is reduced by genotype by environment interactions among the groups of a heterogenous commercial production environment. Key words: Embryo transfer, sire selection, genotype-by-environment interactions

scholarly journals Pregnancy Outcomes After Frozen-Thawed Embryo Transfer in the Absence of a Corpus Luteum

2021 ◽  
Vol 8 ◽  
Author(s):  
Freya Waschkies ◽  
Luka Kroning ◽  
Thilo Schill ◽  
Arvind Chandra ◽  
Cordula Schippert ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Pregnancy Outcomes ◽  
Treatment Protocol ◽  
Neonatal Outcomes ◽  
Corpora Lutea ◽  
Potential Link ◽  
Maternal And Neonatal Outcomes ◽  
Adverse Pregnancy ◽  
Frozen Thawed Embryo Transfer ◽  
Thawed Embryo Transfer

Background: Nowadays, frozen-thawed embryo transfer (FET) cycles represent a high proportion of fertility treatments worldwide. Recent studies suggest differences in pregnancy outcomes depending on the FET treatment protocol used. The reason for this is still unclear, but the number of corpora lutea (CL) at conception is discussed as a possible factor. This study aims to investigate whether maternal and neonatal outcomes for pregnancies following FET lacking a CL differ from FET with one or more CL in order to explore a potential link between CL absence and adverse pregnancy outcomes.Methods: The study was designed as a retrospective, multi-center observational study with two cohorts after singleton live birth [0 CL cohort (FET in a programmed cycle, n = 114) and ≥ 1 CL cohort (FET in a natural or stimulated cycle, n = 68)]. Participants completed a questionnaire on the outcome of pregnancy and birth records were analyzed in a descriptive way. Multivariable logistic and linear regressions were performed in order to explore associations between CL absence and pregnancy outcomes. The strength of the agreement between the information in the survey and the diagnoses extracted from the files was assessed by Cohen's Kappa.Results: The risk of hypertensive disorders of pregnancy was higher after FET in the absence of a CL compared to FET with CL presence (aOR 5.56, 95% CI 1.12 – 27.72). Birthweights and birthweight percentiles were significantly higher in the 0 CL group. CL absence was a predictor of higher birthweight (adjusted coefficient B 179.74, 95% CI 13.03 – 346.44) and higher birthweight percentiles (adjusted coefficient B 10.23, 95%, 95% CI 2.28 – 18.40) particularly in female newborns of the 0 CL cohort. While the strength of the agreement between the reported information in the survey and the actual diagnoses extracted from the files was good for the majority of outcomes of interest it was fair in terms of hypertension (κ = 0.38).Conclusion: This study supports observations suggesting a potential link between a lack of CL at conception and adverse maternal and neonatal outcomes. Further investigations on causes and pathophysiological relationships are yet to be conducted.

scholarly journals 181COMPARISON OF THE PREGNANCY RATES AFTER SYNCHRONIZATION OF OVULATION USING GnRH AND PGF2± IN RECIPIENT DAIRY CATTLE

2004 ◽  
Vol 16 (2) ◽  
pp. 212 ◽  
Author(s):  
T. Nishisouzu ◽  
M. Sugawara ◽  
S. Aoki ◽  
K. Kishida ◽  
M. Moriyoshi ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Pregnancy Rate ◽  
Embryo Transfer ◽  
Fixed Time ◽  
Pregnancy Rates ◽  
Control Group ◽  
Corpora Lutea ◽  
Transfer Program ◽  
Chi Square ◽  
Follicle Diameter

Treatments with GnRH and PGF2α for synchronization of ovulation has resulted in acceptable pregnancy rates after fixed-time artificial insemination in dairy cows without estrus detection. The objective of the present study was to evaluate the practicability of ovulation synchronization (Ovsynch, Pursley JR et al. 1995 Theriogenology 44, 915–923) in dairy cattle using GnRH and PGF2α for the embryo transfer recipients. Dairy cattle (cows; n=100, heifers; n=88) were randomly allocated to one of two groups. The control group (cows; n=45, heifers; n=37) was composed of cows in natural estrus. The ovulation synchronization group (cows; n=55, heifers; n=51) was treated with an intramuscular injection of 100μg of GnRH at a random stage of the estrous cycle. Seven days later, the cattle received PGF2α (Cows; 25–30mg) or PGF2α analog (Heifers; 0.5mg) in order to regress the corpora lutea (CL). Forty-eight hours later, cows and heifers received a second injection of 100μg GnRH. Embryo transfer was carried out 7 days after the second injection of GnRH in the ovsynch group and 7 days after estrus in the control group. The cattle judged to have CL 17mm were classified as acceptable recipients. The size of the follicles and the CL were determined to be of estrus stage and embryo transfer by means of ultrasonography. The mean numbers of follicles and CL were analyzed by ANOVA, while pregnancy rates were analyzed by chi-square test. The results are presented in the Table. The proportion of cows and heifers determined to be acceptable embryo transfers was not different between the control group and the ovsynch group. There were no differences in the proportion of acceptable embryo transfers between the control group and the ovsynch group. Follicle diameter at the time of estrus in the control group (cows; 20.7±0.7mm, heifers; 16.8±0.5mm) were significantly larger than that of the ovsynch group (cows; 18.0±1.0mm, heifers; 14.7±0.2mm) (P&lt;0.05). Although CL diameter at the time of embryo transfer in heifers showed no differences between the control group and the ovsynch group (25.0±1.0mm v. 22.8±1.5mm), The CL diameter of the control cow group was larger than that of the ovsynch group (29.8±0.7mm v. 26.1±1.0mm, P&lt;0.05). However, no differences in pregnancy rate were seen between the control group and the ovsynch group. These results suggest that ovsynch can be effectively applied in an embryo transfer program for cattle. Table 1 Proportion of acceptable embryo transfer recipients and pregnancy rate in dairy cattle in the control ovsynch groups

Sign in / Sign up

Export Citation Format

Share Document