Studies on the pathogenicity of bovine herpesvirus type 5 in sheep1To the memory of our academic father and friend Dr. A. Bartha.1

1999 ◽  
Vol 22 (3) ◽  
pp. 207-220 ◽  
Author(s):  
K Belák ◽  
L Kucsera ◽  
C Ros ◽  
G Kulcsár ◽  
L Makranszki ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 5

scholarly journals Experimental infection of rabbits with a recombinant bovine herpesvirus type 5 (BoHV-5) gI, gE and US9-negative

2009 ◽  
Vol 29 (11) ◽  
pp. 913-918 ◽  
Author(s):  
Alessandra D'Avila Silva ◽  
Ana Cláudia Franco ◽  
Paulo Augusto Esteves ◽  
Fernando Rosado Spilki ◽  
Paulo Michel Roehe
Keyword(s):  
Neurological Disease ◽  
Age Groups ◽  
Clinical Signs ◽  
Bovine Herpesvirus ◽  
Wild Type ◽  
Herpesvirus Type ◽  
Viral Genes ◽  
Bovine Herpesvirus Type 5 ◽  
Two Age Groups

Bovine herpesvirus type 5 (BoHV-5) is a major cause of viral meningoencephalitis in cattle. The expression of different viral proteins has been associated with BoHV-5 neuropathogenesis. Among these, gI, gE and US9 have been considered essential for the production of neurological disease in infected animals. To evaluate the role of gI, gE and US9 in neurovirulence, a recombinant from which the respective genes were deleted (BoHV-5 gI-/gE-/US9-) was constructed and inoculated in rabbits of two age groups (four and eight weeks-old). When the recombinant virus was inoculated through the paranasal sinuses of four weeks-old rabbits, neurological disease was observed and death was the outcome in 4 out of 13 (30.7 %) animals, whereas clinical signs and death were observed in 11/13 (84.6%) of rabbits infected with the parental virus. In eight weeks-old rabbits, the BoHV-5 gI-/gE-/US9- did not induce clinically apparent disease and could not be reactivated after dexamethasone administration, whereas wild type BoHV-5 caused disease in 55.5% of the animals and was reactivated. These findings reveal that the simultaneous deletion of gI, gE and US9 genes did reduce but did not completely abolish the neurovirulence of BoHV-5 in rabbits, indicating that other viral genes may also play a role in the induction of neurological disease.

scholarly journals Evaluation of developmental changes in bovine in vitro produced embryos following exposure to bovine Herpesvirus type 5

2012 ◽  
Vol 10 (1) ◽  
pp. 53 ◽  
Author(s):  
Mariana PC Brenner ◽  
Camila Silva-Frade ◽  
Marina C Ferrarezi ◽  
Andrea F Garcia ◽  
Eduardo F Flores ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Developmental Changes ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 5

Susceptibility of mice to bovine herpesvirus type 5 infection in the central nervous system

2017 ◽  
Vol 41 (4) ◽  
pp. 279-288 ◽  
Author(s):  
L. P. Mesquita ◽  
R. C. Costa ◽  
M. M. Fusuma ◽  
F. R. P. Bruhn ◽  
E. Mori ◽  
...  
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
Bovine Herpesvirus ◽  
Herpesvirus Type ◽  
The Central Nervous System ◽  
Bovine Herpesvirus Type 5

In vitroantiviral activity of Brazilian plants (Maytenus ilicifoliaandAniba rosaeodora) against bovine herpesvirus type 5 and avian metapneumovirus

2012 ◽  
Vol 50 (10) ◽  
pp. 1269-1275 ◽  
Author(s):  
L. K. Kohn ◽  
C. L. Queiroga ◽  
M. C. Martini ◽  
L. E. Barata ◽  
P. S. S. Porto ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Avian Metapneumovirus ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 5

scholarly journals Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine

2018 ◽  
Vol 70 (2) ◽  
pp. 375-381 ◽  
Author(s):  
T.B. Roos ◽  
L.F.C. Avila ◽  
R.T. Sturbelle ◽  
F.L.L. Leite ◽  
G. Fischer ◽  
...  
Keyword(s):  
Immune Response ◽  
Animal Health ◽  
Bovine Herpesvirus ◽  
Saccharomyces Boulardii ◽  
Igg Antibody ◽  
Th1 Response ◽  
Herpesvirus Type ◽  
Cell Mediated Immune Response ◽  
A Cell ◽  
Bovine Herpesvirus Type 5

ABSTRACT There have been significant efforts towards the development of more efficient vaccines for animal health. A strategy that may be used to improve vaccine efficacy is the use of probiotics to enhance the immune response of the host, leading to increased immunogenicity of antigen preparations. Bovine herpesvirus 5 (BoHV-5) is an example of an important animal pathogen for which vaccines have provided only limited protection. In this study, we examined the use of the probiotic Saccharomyces boulardii (Sb) as a potential adjuvant to improve vaccine efficiency. We found that the supplemented animals exhibited an enhanced systemic IgG antibody response toward a Th1 response in favor of IgG2a and increased mRNA expression levels of the cytokines IFN-y, IL-12, IL-17 and IL-10 in the spleen. These results suggest that Sb supplementation may provide a promising means for improving the efficiency of vaccines, particularly those that rely on a cell-mediated immune response.

Latency of bovine herpesvirus type 5 (BoHV-5) in tonsils and peripheral blood leukocytes

2014 ◽  
Vol 202 (1) ◽  
pp. 134-140 ◽  
Author(s):  
P.A. Favier ◽  
M.S. Marin ◽  
P.E. Morán ◽  
A.C. Odeón ◽  
A.E. Verna ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Bovine Herpesvirus ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Herpesvirus Type ◽  
Bovine Herpesvirus Type 5

scholarly journals Immunogenicity of an inactivated bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E

2010 ◽  
Vol 30 (1) ◽  
pp. 57-62 ◽  
Author(s):  
Mário Celso S. Brum ◽  
Luizinho Caron ◽  
Shafiqul I. Chowdhury ◽  
Rudi Weiblen ◽  
Eduardo Furtado Flores
Keyword(s):  
Thymidine Kinase ◽  
Recombinant Virus ◽  
Vaccine Dose ◽  
Bovine Herpesvirus ◽  
Serological Response ◽  
Serum Samples ◽  
Glycoprotein E ◽  
Herpesvirus Type ◽  
Negative Results ◽  
Bovine Herpesvirus Type 5

The immunogenicity of an inactivated, experimental vaccine based on a bovine herpesvirus type 5 strain defective in thymidine kinase and glycoprotein E (BoHV-5 gE/TKΔ) was evaluated in cattle and the results were compared with a vaccine containing the parental BoHV-5 strain (SV507/99). To formulate the vaccines, each virus (wildtype SV507/99 and BoHV-5 gE/TK∆) was multiplied in cell culture and inactivated with binary ethyleneimine (BEI). Each vaccine dose contained approximately of 10(7.5) TCID50 of inactivated virus mixed with an oil-based adjuvant (46:54). Forty calves, 6 to 9-months-old, were allocated into two groups of 20 animals each and vaccinated twice (days 0 and 22pv) by the subcutaneous route with either vaccine. Serum samples collected at day 0 and at different intervals after vaccination were tested for virus neutralizing (VN) antibodies against the parental virus and against heterologous BoHV-5 and BoHV-1 isolates. The VN assays demonstrated seroconversion to the respective homologous viruses in all vaccinated animals after the second vaccine dose (mean titers of 17.5 for the wildtype vaccine; 24.1 for the recombinant virus). All animals remained reagents up to day 116 pv, yet showing a gradual reduction in VN titers. Animals from both vaccine groups reacted in similar VN titers to different BoHV-1 and BoHV-5 isolates, yet the magnitude of serological response of both groups was higher against BoHV-5 field isolates. Calves vaccinated with the recombinant virus did not develop antibodies to gE as verified by negative results in a gE-specific ELISA, what would allow serological differentiation from naturally infected animals. Taken together, these results indicate that inactivated antigens of BoHV-5 gE/TK recombinant virus induced an adequate serological response against BoHV-5 and BoHV-1 and thus can be used as an alternative, differential vaccine candidate.

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