Expression and genome-wide distribution of the gene family encoding a 90 kDa surface glycoprotein of metacyclic trypomastigotes of Trypanosoma cruzi

2002 ◽  
Vol 125 (1-2) ◽  
pp. 201-206 ◽  
Author(s):  
Mirian Silva do Carmo ◽  
Marcia Regina Machado dos Santos ◽  
Maria Isabel Cano ◽  
Jorge Enrique Araya ◽  
Nobuko Yoshida ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Gene Family ◽  
Wide Distribution ◽  
Surface Glycoprotein ◽  
Metacyclic Trypomastigotes ◽  
Genome Wide

scholarly journals Infection by Trypanosoma cruzi Metacyclic Forms Deficient in gp82 but Expressing a Related Surface Molecule, gp30

2003 ◽  
Vol 71 (11) ◽  
pp. 6184-6191 ◽  
Author(s):  
Mauro Cortez ◽  
Ivan Neira ◽  
Daniele Ferreira ◽  
Alejandro O. Luquetti ◽  
Anis Rassi ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Cell Invasion ◽  
Target Cell ◽  
Surface Profile ◽  
Cell Entry ◽  
Gastric Mucin ◽  
Surface Glycoprotein ◽  
Metacyclic Trypomastigotes ◽  
Mucosal Infection

ABSTRACT Trypanosoma cruzi metacyclic trypomastigotes invade and replicate in the gastric mucosal epithelium after oral infection. In this study we analyzed the process of infection by T. cruzi isolates deficient in the expression of gp82, the metacyclic stage-specific surface glycoprotein implicated in target cell entry in vitro and in promoting mucosal infection in mice after oral challenge. Mice infected by the oral route with metacyclic forms of gp82-deficient isolate 569 or 588 developed patent parasitemia but at greatly reduced levels compared to those infected with the gp82-expressing isolate CL. Metacyclic forms of both isolates expressed gp30, a surface glycoprotein detectable by monoclonal antibody (MAb) 3F6 directed to gp82. Otherwise, the gp82-deficient isolates displayed a surface profile similar to that of the CL isolate and also entered epithelial HeLa cells in a manner inhibitable by MAb 3F6 and dependent on the parasite signal transduction that involved the activation of protein tyrosine kinase and Ca2+ mobilization from thapsigargin-sensitive stores. Like gp82, gp30 triggered the host cell Ca2+ response required for parasite internalization. Purified gp30 and the recombinant gp82 inhibited HeLa cell invasion of metacyclic forms of isolates 569 and 588 by ∼90 and ∼70%, respectively. A cell invasion assay performed in the presence of gastric mucin, mimicking the in vivo infection, showed an inhibition of 70 to 75% in the internalization of gp82-deficient isolates but not of the CL isolate. The recombinant gp82 exhibited an adhesive capacity toward gastric mucin much higher than that of gp30. Taken together, our findings indicate that target cell entry of metacyclic trypomastigotes can be mediated either by gp82 or gp30 but that efficient mucosal infection depends on the expression of gp82.

scholarly journals Structure and transcription of genes encoding the surface glycoprotein antigens gp90 and gp82 of Trypanosoma cruzi metacyclic trypomastigotes

2000 ◽  
Vol 72 (1) ◽  
pp. 104-105
Author(s):  
Mirian S. Carmo ◽  
Jorge E Araya ◽  
Maria I. Cano ◽  
Marcel I. Ramirez ◽  
Renata P. Baida ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Surface Glycoprotein ◽  
Metacyclic Trypomastigotes ◽  
Genes Encoding

scholarly journals Conservation, Divergence, and Genome-Wide Distribution ofPALandPOX AGene Families in Plants

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
H. C. Rawal ◽  
N. K. Singh ◽  
T. R. Sharma
Keyword(s):  
Gene Family ◽  
Populus Trichocarpa ◽  
Brachypodium Distachyon ◽  
Functional Divergence ◽  
Functional Characterization ◽  
Gene Families ◽  
Wide Distribution ◽  
Genome Wide ◽  
High Level ◽  
Diverse Groups

Genome-wide identification and phylogenetic and syntenic comparison were performed for the genes responsible for phenylalanine ammonia lyase (PAL) and peroxidase A (POX A) enzymes in nine plant species representing very diverse groups like legumes (Glycine maxandMedicago truncatula), fruits (Vitis vinifera), cereals (Sorghum bicolor,Zea mays, andOryza sativa), trees (Populus trichocarpa), and model dicot (Arabidopsis thaliana) and monocot (Brachypodium distachyon) species. A total of 87 and 1045 genes in PAL and POX A gene families, respectively, have been identified in these species. The phylogenetic and syntenic comparison along with motif distributions shows a high degree of conservation of PAL genes, suggesting that these genes may predate monocot/eudicot divergence. The POX A family genes, present in clusters at the subtelomeric regions of chromosomes, might be evolving and expanding with higher rate than the PAL gene family. Our analysis showed that during the expansion of POX A gene family, many groups and subgroups have evolved, resulting in a high level of functional divergence among monocots and dicots. These results will act as a first step toward the understanding of monocot/eudicot evolution and functional characterization of these gene families in the future.

scholarly journals Genome-Wide Identification and Analysis of Chitinase-Like Gene Family in Bemisia tabaci (Hemiptera: Aleyrodidae)

Insects ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 254
Author(s):  
Zhengke Peng ◽  
Jun Ren ◽  
Qi Su ◽  
Yang Zeng ◽  
Lixia Tian ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Patterns ◽  
Wide Distribution ◽  
Developmental Expression ◽  
Glycoside Hydrolase Family ◽  
Genome Wide ◽  
And Function ◽  
Disk Growth ◽  
Hydrolase Family

Chitinases are of great importance in chitin degradation and remodeling in insects. However, the genome-wide distribution of chitinase-like gene family in Bemsia tabaci, a destructive pest worldwide, is still elusive. With the help of bioinformatics, we annotated 14 genes that encode putative chitinase-like proteins, including ten chitinases (Cht), three imaginal disk growth factors (IDGF), and one endo-β-N-acetylglucosaminidase (ENGase) in the genome of the whitefly, B. tabaci. These genes were phylogenetically grouped into eight clades, among which 13 genes were classified in the glycoside hydrolase family 18 groups and one in the ENGase group. Afterwards, developmental expression analysis suggested that BtCht10, BtCht5, and BtCht7 were highly expressed in nymphal stages and exhibit similar expression patterns, implying their underlying role in nymph ecdysis. Notably, nymphs exhibited a lower rate of survival when challenged by dsRNA targeting these three genes via a nanomaterial-promoted RNAi method. In addition, silencing of BtCht10 significantly resulted in a longer duration of development compared to control nymphs. These results indicate a key role of BtCht10, BtCht5, and BtCht7 in B. tabaci nymph molting. Our research depicts the differences of chitinase-like family genes in structure and function and identified potential targets for RNAi-based whitefly management.

Genome-wide identification and analysis of maize PAL gene family and its expression profile in response to high-temperature stress

2020 ◽  
Vol 52 (5) ◽  
Author(s):  
De-Gong Wu ◽  
Qiu-Wen Zhan ◽  
Hai-Bing Yu ◽  
Bao-Hong Huang ◽  
Xin-Xin Cheng ◽  
...  
Keyword(s):  
High Temperature ◽  
Gene Family ◽  
Temperature Stress ◽  
Expression Profile ◽  
High Temperature Stress ◽  
Genome Wide

Genome-wide Analysis and Expression Profiling of SPS Gene Family in Brassica nupus L.

2018 ◽  
Vol 44 (2) ◽  
pp. 197
Author(s):  
Li ZHANG ◽  
Hong-Ju JIAN ◽  
Bo YANG ◽  
Ao-Xiang ZHANG ◽  
Chao ZHANG ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Profiling ◽  
Genome Wide Analysis ◽  
Genome Wide

Genome-Wide Identification, Classification, and Expression of NF-YB Gene Family in Soybean

2013 ◽  
Vol 38 (9) ◽  
pp. 1570-1582
Author(s):  
Wei-Jun ZHENG ◽  
Zhao-Shi XU ◽  
Zhi-Juan FENG ◽  
Lian-Cheng LI ◽  
Ming CHEN ◽  
...  
Keyword(s):  
Gene Family ◽  
Genome Wide

Genome-wide Characterization of Major Intrinsic Protein (MIP) Gene Family in Brachypodium distachyon

2018 ◽  
Vol 13 (5) ◽  
pp. 536-552 ◽  
Author(s):  
Ankush Ashok Saddhe ◽  
Shweta ◽  
Kareem A. Mosa ◽  
Kundan Kumar ◽  
Manoj Prasad ◽  
...  
Keyword(s):  
Gene Family ◽  
Brachypodium Distachyon ◽  
Major Intrinsic Protein ◽  
Intrinsic Protein ◽  
Genome Wide

scholarly journals Genome-wide identification of cyclin-dependent kinase (CDK) genes affecting adipocyte differentiation in cattle

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Cuili Pan ◽  
Zhaoxiong Lei ◽  
Shuzhe Wang ◽  
Xingping Wang ◽  
Dawei Wei ◽  
...  
Keyword(s):  
Gene Family ◽  
Expression Analysis ◽  
Adipocyte Differentiation ◽  
Bos Taurus ◽  
Adipogenic Differentiation ◽  
Critical Role ◽  
Bos Indicus ◽  
Specific Expression ◽  
Genome Wide ◽  
A Genome

Abstract Background Cyclin-dependent kinases (CDKs) are protein kinases regulating important cellular processes such as cell cycle and transcription. Many CDK genes also play a critical role during adipogenic differentiation, but the role of CDK gene family in regulating bovine adipocyte differentiation has not been studied. Therefore, the present study aims to characterize the CDK gene family in bovine and study their expression pattern during adipocyte differentiation. Results We performed a genome-wide analysis and identified a number of CDK genes in several bovine species. The CDK genes were classified into 8 subfamilies through phylogenetic analysis. We found that 25 bovine CDK genes were distributed in 16 different chromosomes. Collinearity analysis revealed that the CDK gene family in Bos taurus is homologous with Bos indicus, Hybrid-Bos taurus, Hybrid Bos indicus, Bos grunniens and Bubalus bubalis. Several CDK genes had higher expression levels in preadipocytes than in differentiated adipocytes, as shown by RNA-seq analysis and qPCR, suggesting a role in the growth of emerging lipid droplets. Conclusion In this research, 185 CDK genes were identified and grouped into eight distinct clades in Bovidae, showing extensively homology. Global expression analysis of different bovine tissues and specific expression analysis during adipocytes differentiation revealed CDK4, CDK7, CDK8, CDK9 and CDK14 may be involved in bovine adipocyte differentiation. The results provide a basis for further study to determine the roles of CDK gene family in regulating adipocyte differentiation, which is beneficial for beef quality improvement.

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