PH-0550 Importance of high thermal dose in post-operative re-irradiation and hyperthermia in breast cancer

Objective: In this study, the hyperthermia effect on the viability of human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells was evaluated by MTT assay. Methods: Cells were exposed to heat at 38ºC, 39ºC, 40ºC, 41ºC, 42ºC, 43ºC, and 44ºC for five different durations of heat exposure (0.5, 1, 2, 3, and 4 h). Breakpoint temperatures of MCF-10A, MDA-MB 231, and MCF-7 were determined using cumulative equivalent 43°C (CEM43) model. This model was first time used to calculate thermal isoeffect dose (TID) for MCF-10A, MDA-MB 231, and MCF-7. Results: MCF-10A started to die at 42°C for 3 h while MDA-MB 231 and MCF-7 need a temperature of 38°C for 0.5 h; thus, they were identified as the threshold temperatures in CEM43 model. Furthermore, the effect of “43°C incubator 2 h” had similar total thermal dose as “44°C incubator 0.5 h” for MDA-MB 231 and MCF-7. In addition, “43°C incubator 3 h” effect had also almost the same thermal dose as “44°C incubator 1 h” for MDA-MB 231 and MCF-7. Conclusion: A better understanding of the significant correlations between CEM43 and response parameters in clinical trials could be useful to treat breast cancer patients.

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Temperature and thermal dose during radiotherapy and hyperthermia for recurrent breast cancer are related to clinical outcome and thermal toxicity: a systematic review

International Journal of Hyperthermia ◽

10.1080/02656736.2019.1665718 ◽

2019 ◽

Vol 36 (1) ◽

pp. 1023-1038 ◽

Cited By ~ 17

Author(s):

Akke Bakker ◽

Jacoba van der Zee ◽

Geertjan van Tienhoven ◽

H. Petra Kok ◽

Coen R. N. Rasch ◽

...

Keyword(s):

Breast Cancer ◽

Systematic Review ◽

Clinical Outcome ◽

Recurrent Breast Cancer ◽

Thermal Dose ◽

Recurrent Breast

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Relationship between thermal dose and outcome in thermoradiotherapy treatments for superficial recurrences of breast cancer: Data from a phase III trial

International Journal of Radiation Oncology*Biology*Physics ◽

10.1016/s0360-3016(97)00333-7 ◽

1997 ◽

Vol 39 (2) ◽

pp. 371-380 ◽

Cited By ~ 115

Author(s):

Michael Sherar ◽

Fei-Fei Liu ◽

Melania Pintilie ◽

Wilfred Levin ◽

John Hunt ◽

...

Keyword(s):

Breast Cancer ◽

Phase Iii ◽

Thermal Dose ◽

Breast Cancer Data ◽

Phase Iii Trial ◽

Cancer Data

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Focused microwave phased array thermotherapy for ablation of early-stage breast cancer: results of thermal dose escalation

The Women s Oncology Review ◽

10.1080/1473340410001732352 ◽

2004 ◽

Vol 4 (2) ◽

pp. 145-146

Keyword(s):

Breast Cancer ◽

Dose Escalation ◽

Phased Array ◽

Early Stage ◽

Early Stage Breast Cancer ◽

Thermal Dose

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Virus-Like Particles in a Human Breast Cancer: Structural Similarity to Previously Reported Particles

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100072332 ◽

1973 ◽

Vol 31 ◽

pp. 378-379

Author(s):

G. Kasnic ◽

S. E. Stewart ◽

C. Urbanski

Keyword(s):

Breast Cancer ◽

Biological Activity ◽

Human Breast Cancer ◽

Osteogenic Sarcoma ◽

Human Tumor ◽

Structural Similarity ◽

Cell Tumor ◽

Human Breast ◽

Human Tumor Cell ◽

Type Particle

We have reported the maturation of an intracisternal A-type particle in murine plasma cell tumor cultures and three human tumor cell cultures (rhabdomyosarcoma, lung adenocarcinoma, and osteogenic sarcoma) after IUDR-DMSO activation. In all of these studies the A-type particle seems to develop into a form with an electron dense nucleoid, presumably mature, which is also intracisternal. A similar intracisternal A-type particle has been described in leukemic guinea pigs. Although no biological activity has yet been demonstrated for these particles, on morphologic grounds, and by the manner in which they develop within the cell, they may represent members of the same family of viruses.

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Further Ultrastructural Observations on Metastatic Nodules of Human Breast Cancer

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010010086x ◽

1968 ◽

Vol 26 ◽

pp. 224-225

Author(s):

John L. Swedo ◽

R. W. Talley ◽

John H. L. Watson

Keyword(s):

Breast Cancer ◽

Human Breast Cancer ◽

Estrogen Therapy ◽

Specimen Preparation ◽

Human Breast ◽

Autophagic Vacuoles ◽

Previous Communication ◽

Linear Profiles ◽

Metastatic Nodule

Since the report, which described the ultrastructure of a metastatic nodule of human breast cancer after estrogen therapy, additional ultrastructural observations, including some which are correlative with pertinent findings in the literature concerning mycoplasmas, have been recorded concerning the same subject. Specimen preparation was identical to that in.The mitochondria possessed few cristae, and were deteriorated and vacuolated. They often contained particulates and fibrous structures, sometimes arranged in spindle-shaped bundles, Fig. 1. Another apparent aberration was the occurrence, Fig. 2 (arrows) of linear profiles of what seems to be SER, which lie between layers of RER, and are often recognizably continuous with them.It was noted that the structure of the round bodies, interpreted as within autophagic vacuoles in the previous communication, and of vesicular bodies, described morphologically closely resembled those of some mycoplasmas. Specifically, they simulated or reflected the various stages of replication reported for mycoplasmas grown on solid nutrient. Based on this observation, they are referred to here as “mycoplasma-like” structures, in anticipation of confirmatory evidence from investigations now in progress.

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