Presence of hepatitis B surface antigen mutant G145R DNA in the peripheral blood leukocytes of the family members of an asymptomatic carrier and evidence of its horizontal transmission

2002 ◽  
Vol 90 (1-2) ◽  
pp. 133-141 ◽  
Author(s):  
Runu Chakravarty ◽  
Madhubanti Neogi ◽  
Susanta Roychowdhury ◽  
Chinmoy Kumar Panda
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Peripheral Blood ◽  
Asymptomatic Carrier ◽  
Hepatitis B Surface Antigen ◽  
Horizontal Transmission ◽  
Family Members ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
The Family

HEPATITIS B SURFACE ANTIGEN AND CHRONIC HEPATITIS IN INFANTS BORN TO ASYMPTOMATIC CARRIER MOTHERS

1978 ◽  
Vol 33 (3) ◽  
pp. 169
Author(s):  
Kazuo Shiraki ◽  
Namiko Yoshihara ◽  
Takashi Kawana ◽  
Hiroshi Yasui ◽  
Michiro Sakurai
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Asymptomatic Carrier ◽  
Hepatitis B Surface Antigen

scholarly journals Genetic Characterization of Hepatitis B Virus in Peripheral Blood Leukocytes: Evidence for Selection and Compartmentalization of Viral Variants with the Immune Escape G145R Mutation

2009 ◽  
Vol 83 (19) ◽  
pp. 9983-9992 ◽  
Author(s):  
Sibnarayan Datta ◽  
Rajesh Panigrahi ◽  
Avik Biswas ◽  
Partha K. Chandra ◽  
Arup Banerjee ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Peripheral Blood ◽  
Immune Escape ◽  
Escape Mutant ◽  
Large Set ◽  
Peripheral Blood Leukocytes ◽  
Blood Leukocytes ◽  
Frequent Event ◽  
B Virus

ABSTRACT The compartmentalization of viral variants in distinct host tissues is a frequent event in many viral infections. Although hepatitis B virus (HBV) classically is considered hepatotropic, it has strong lymphotropic properties as well. However, unlike other viruses, molecular evolutionary studies to characterize HBV variants in compartments other than hepatocytes or sera have not been performed. The present work attempted to characterize HBV sequences from the peripheral blood leukocytes (PBL) of a large set of subjects, using advanced molecular biology and computational methods. The results of this study revealed the exclusive compartmentalization of HBV subgenotype Ae/A2-specific sequences with a potent immune escape G145R mutation in the PBL of the majority of the subjects. Interestingly, entirely different HBV genotypes/subgenotypes (C, D, or Aa/A1) were found to predominate in the sera of the same study populations. These results suggest that subgenotype Ae/A2 is selectively archived in the PBL, and the high prevalence of G145R indicates high immune pressure and high evolutionary rates of HBV DNA in the PBL. The results are analogous to available literature on the compartmentalization of other viruses. The present work thus provides evidence in favor of the compartment-specific abundance, evolution, and emergence of the potent immune escape mutant. These findings have important implications in the field of HBV molecular epidemiology, transmission, transfusion medicine, organ transplantation, and vaccination strategies.

Activation of human peripheral blood B cells following immunization with hepatitis B surface antigen vaccine

1984 ◽  
Vol 86 (1) ◽  
pp. 145-154 ◽  
Author(s):  
Thomas R. Cupps ◽  
Paul K. Goldsmith ◽  
David J. Volkman ◽  
John L. Gerin ◽  
Robert H. Purcell ◽  
...  
Keyword(s):  
B Cells ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Hepatitis B Surface Antigen ◽  
Antigen Vaccine

Hepatitis B surface antigen in the serum of infants after delivery from asymptomatic carrier mothers

1975 ◽  
Vol 87 (3) ◽  
pp. 360-363 ◽  
Author(s):  
Kiyoshi Okada ◽  
Tetsuo Yamada ◽  
Yuzo Miyakawa ◽  
Makoto Mayumi
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Asymptomatic Carrier ◽  
Hepatitis B Surface Antigen

Possible transmission of hepatitis B virus between spouses and their children in Babol, Northern Iran

2007 ◽  
Vol 37 (4) ◽  
pp. 245-247 ◽  
Author(s):  
Mohammad Reza Hasanjani Roushan ◽  
Minoo Mohraz ◽  
Ali Akbar Velayati
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Hepatitis B Surface Antigen ◽  
Family Members ◽  
Hbv Infection ◽  
Hepatitis B E Antigen ◽  
Northern Iran ◽  
Hbv Markers ◽  
B Virus

To determine the possible routes of transmission of hepatitis B virus (HBV) infection between spouses and their children in Babol, Northern Iran, the spouses of 54 infected husbands and 49 wives without any evidence of HBV infection in their family members were evaluated from March 1998 to April 2005. All of these cases were hepatitis B surface antigen and anti-hepatitis B 'e' antigen positive. Mean duration of marriage for husbands was 14.4±6.49 and for wives12.46±6.24 years. Past HBV infection was found in 46.3% wives of infected husbands and in 65.3% husbands of infected wives ( P = 0.074). HBV markers were seen in 32.5% children of infected fathers and 64.5% children of infected mothers ( P = 0.0001). HBV carrier rates in boys and girls of infected mothers were significantly higher than in those of infected fathers ( P = 0.002 and P = 0.0001, respectively). The results show that transmission of HBV between spouses and their children were the main routes of transmission of HBV in our region.

scholarly journals The Study of Correlation between Serum Vitamin D3 Concentrations and HBV DNA Levels and Immune Response in Chronic Hepatitis Patients

Nutrients ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 1114
Author(s):  
Wang-Sheng Ko ◽  
Yen-Ping Yang ◽  
Fang-Ping Shen ◽  
Mu-Chen Wu ◽  
Chia-Ju Shih ◽  
...  
Keyword(s):  
Chronic Hepatitis ◽  
Viral Load ◽  
Hepatitis B ◽  
Surface Antigen ◽  
Vitamin D3 ◽  
Peripheral Blood ◽  
Hepatitis B Surface Antigen ◽  
Serum Vitamin ◽  
Hbv Dna ◽  
Hbv Infection

Chronic hepatitis B (CHB) is a common chronic disease. Previous studies have shown a link between 25-hydroxyvitamin D3 (vitamin D3) concentration and liver disease. Hepatitis B virus (HBV) infection has been attributed to the inappropriate functioning of cell-mediated immunity. However, the effects of vitamin D3, immune cell, and HBeAg status on HBV viral load in CHB patients are still unclear. We investigated the relationship between the serum concentration of vitamin D3, percentage of immune cells in peripheral blood, and the HBV viral load of CHB patients. Sixty CHB patients were recruited, and their blood samples were collected and analyzed. Vitamin D level was measured using a chemiluminescence assay. A level of 30 ng/mL or above was defined as a vitamin D3 sufficiency. We assigned vitamin D3 status as either normal (≥30 ng/mL), insufficient (20–30 ng/mL), or deficient (<20 ng/mL). T-lymphocyte and B-lymphocyte surface markers in peripheral blood were detected using flow cytometry. The factors associated with HBV viral load were analyzed using univariate and multivariate-adjusted models. The mean serum vitamin D3 concentration in the subjects was 20.9 ± 5.6 ng/mL. Up to 88.3% of the patients were either deficient in or had insufficient vitamin D3. The gender, BMI, hepatitis B surface antigen levels, and ALT levels were significantly related to serum vitamin D3 levels. Serum vitamin D3 concentration, HBe status, HBs levels, ALT, and AST levels showed a statistically significant correlation with the HBV DNA levels. Serum vitamin D3 concentrations and hepatitis B surface antigen levels were strongly correlated with HBV DNA levels. Vitamin D3 levels were significantly associated with CD19 numbers (β:−6.2, 95% CI: −10.5). In multivariate analysis, vitamin D3 levels in the deficient and insufficient groups, and the CD8, HBeAg, and WBC counts were significantly associated with HBV DNA levels. In the immune tolerance phase of HBeAg-negative chronic HBV infection, vitamin D3 may be a modulator of immune function via CD8, CD19, and HBV DNA.

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