430 Association of immune cell infiltration and hepatic interferon-stimulated gene expression in the absence of IRF-3 activation in chronic hepatitis C

Cancer immunotherapy has emerged as an effective therapy in a variety of cancers. However, a key challenge in the field is that only a subset of patients who receive immunotherapy exhibit durable response. It has been hypothesized that host genetics influences the inherent immune profiles of patients and may underlie their differential response to immunotherapy. Herein, we systematically determined the association of common germline genetic variants with gene expression and immune cell infiltration of the tumor. We identified 64,094 expression quantitative trait loci (eQTLs) that associated with 18,210 genes (eGenes) across 24 human cancers. Overall, eGenes were enriched for their being involved in immune processes, suggesting that expression of immune genes can be shaped by hereditary genetic variants. We identified the endoplasmic reticulum aminopeptidase 2 (ERAP2) gene as a pan-cancer type eGene whose expression levels stratified overall survival in a subset of patients with bladder cancer receiving anti–PD-L1 (atezolizumab) therapy. Finally, we identified 103 gene signature QTLs (gsQTLs) that were associated with predicted immune cell abundance within the tumor microenvironment. Our findings highlight the impact of germline SNPs on cancer-immune phenotypes and response to therapy; and these analyses provide a resource for integration of germline genetics as a component of personalized cancer immunotherapy.

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Gene expression profiles for predicting treatment response in patients with chronic hepatitis C: Preliminary analysis

Digestive and Liver Disease ◽

10.1016/j.dld.2006.12.078 ◽

2007 ◽

Vol 39 (3) ◽

pp. A22

Author(s):

A. Ciancio ◽

E. Medico ◽

A. Olivero ◽

M. Berrutti ◽

P. Caviglia ◽

...

Keyword(s):

Gene Expression ◽

Chronic Hepatitis ◽

Hepatitis C ◽

Treatment Response ◽

Chronic Hepatitis C ◽

Preliminary Analysis ◽

Expression Profiles ◽

Gene Expression Profiles

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Colon Cancer and SARS-CoV-2: Impact of ACE2 Expression in Susceptibility to COVID-19

10.1101/2020.06.11.146878 ◽

2020 ◽

Cited By ~ 1

Author(s):

Mohsen Ahmadi ◽

Negin Saffarzadeh ◽

Mohammad Amin Habibi ◽

Fatemeh Hajiesmaeili ◽

Nima Rezaei

Keyword(s):

Gene Expression ◽

Colon Cancer ◽

Immune Cell ◽

Expression Patterns ◽

Methylation Status ◽

Functional Enrichment ◽

Cell Infiltration ◽

Survival Outcomes ◽

Immune Cell Infiltration ◽

Ace2 Gene

AbstractNovel coronavirus disease (COVID-19) pandemic has become a global health emergency. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) interacts with angiotensin-converting enzyme 2 (ACE2) to enter the cells and infects diverse human tissues. It has been reported that a few conditions, including cancer, predispose individuals to SARS-CoV-2 infection and severe form of COVID-19. These findings led us to evaluate the susceptibility of colon adenocarcinoma (COAD) patients to SARS-CoV-2 infection by investigation of ACE2 expression in their tumor tissues. The expression analysis revealed that both mRNA and protein levels of ACE2 had increased in colon cancer samples than normal group. Next, the prognosis analysis has indicated that the upregulation of ACE2 was not correlated with patient survival outcomes. Further assessment displayed the hypomethylation of the ACE2 gene promoter in COAD patients. Surprisingly, this methylation status has a strong negative correlation with ACE2 gene expression. The functional enrichment analysis of the genes that had similar expression patterns with ACE2 in colon cancer tissues demonstrated that they mainly enriched in Vitamin digestion and absorption, Sulfur relay system, and Fat digestion and absorption pathways. Finally, we found that ACE2 gene expression had a significant association with the immune cell infiltration levels in COAD patients. In conclusion, it has plausible that COAD patients are more likely to be infected with SARS-CoV-2 and experience severe injuries. Moreover, COVID-19 would bring unfavorable survival outcomes of patients with colon cancer by the way of immune cell infiltration linked process. The present study highlights the importance of preventive actions for COAD patients during the COVID-19 pandemic.

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Interferon type I gene expression in chronic hepatitis C

Laboratory Investigation ◽

10.1038/labinvest.3700135 ◽

2004 ◽

Vol 84 (9) ◽

pp. 1148-1159 ◽

Cited By ~ 67

Author(s):

Sabine Mihm ◽

Michael Frese ◽

Volker Meier ◽

Perdita Wietzke-Braun ◽

Jens-Gerd Scharf ◽

...

Keyword(s):

Gene Expression ◽

Chronic Hepatitis ◽

Hepatitis C ◽

Chronic Hepatitis C ◽

Type I ◽

I Gene

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Cell surface expression of LDL receptor in chronic hepatitis C: correlation with viral load

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00091.2007 ◽

2007 ◽

Vol 293 (1) ◽

pp. E416-E420 ◽

Cited By ~ 22

Author(s):

Jean-Michel Petit ◽

Anne Minello ◽

Laurence Duvillard ◽

Valérie Jooste ◽

Serge Monier ◽

...

Keyword(s):

Gene Expression ◽

Chronic Hepatitis ◽

Viral Load ◽

Hepatitis C ◽

Cell Surface ◽

Chronic Hepatitis C ◽

Mononuclear Cells ◽

Ldl Receptor ◽

Surface Expression ◽

Cell Surface Expression

The LDL receptor (LDL-R) has been proposed as the viral receptor for Hepatitis C virus (HCV). This hypothesis has been based exclusively on in vitro studies. In human mononuclear cells, LDL-R gene expression has been demonstrated to be parallel and be coordinately regulated to gene expression in the human liver. The purpose of the current study was to determine the mononuclear cell surface expression of the LDL receptor in patients with HCV chronic infection according to viral load. Sixty-eight consecutive untreated chronic hepatitis C patients were studied to determine the mononuclear cell surface expression of the LDL-R. LDL-Rs were quantified at the surface of mononuclear cells in fresh blood samples taken after fasting using flow cytometry. LDL-R expression was significantly associated with LDL-cholesterol ( r = −0.25; P = 0.03) and HCV-viral load ( r = 0.37, P = 0.002). In multivariate analysis, the LDL-R expression was significantly associated with HCV viral load, whereas genotype, age, body mass index, and fibrosis were not. In conclusion, our data provided by a human study, suggest that the LDL-R may be one of the receptors implicated in HCV replication.

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