Osmotic stress induced changes in lipid composition and peroxidation in leaf discs of Brassica napus L.

The effect of increasing NaCl concentrations up to 170 mM on the lipid composition of leaf membranes was studied on rape (Brassica napus L. var. oleifera Metzg) in hydroponic culture for one month. At NaCl concentrations lower than 102 mM, the polar glycerolipid biosynthesis was stimulated. NaCl concentrations higher than 102 mM reduced the contents in total lipids, monogalactosyldiacylglycerol, digalactosyldiacylglycerol, and phospholipids. In contrast, the neutral lipid contents increased with increasing NaCl concentration. An increase in NaCl induced a decrease in linolenic and hexadecatrienoic acid contents and an increase in linoleic and palmitic acid contents in all lipid groups. Key words: rape, lipids, sodium chloride.

Download Full-text

The effect of simulated microgravity on the Brassica napus seedling proteome

Functional Plant Biology ◽

10.1071/fp16378 ◽

2018 ◽

Vol 45 (4) ◽

pp. 440 ◽

Cited By ~ 4

Author(s):

Andrej Frolov ◽

Anna Didio ◽

Christian Ihling ◽

Veronika Chantzeva ◽

Tatyana Grishina ◽

...

Keyword(s):

Brassica Napus ◽

Simulated Microgravity ◽

Seedling Development ◽

Database Search ◽

Complete Understanding ◽

Brassica Napus L ◽

Proteomic Data ◽

Microgravity Conditions ◽

Induced Changes ◽

Transcriptome Level

The magnitude and the direction of the gravitational field represent an important environmental factor affecting plant development. In this context, the absence or frequent alterations of the gravity field (i.e. microgravity conditions) might compromise extraterrestrial agriculture and hence space inhabitation by humans. To overcome the deleterious effects of microgravity, a complete understanding of the underlying changes on the macromolecular level is necessary. However, although microgravity-related changes in gene expression are well characterised on the transcriptome level, proteomic data are limited. Moreover, information about the microgravity-induced changes in the seedling proteome during seed germination and the first steps of seedling development is completely missing. One of the valuable tools to assess gravity-related issues is 3D clinorotation (i.e. rotation in two axes). Therefore, here we address the effects of microgravity, simulated by a two-axial clinostat, on the proteome of 24- and 48-h-old seedlings of oilseed rape (Brassica napus L.). The liquid chromatography-MS-based proteomic analysis and database search revealed 95 up- and 38 downregulated proteins in the tryptic digests obtained from the seedlings subjected to simulated microgravity, with 42 and 52 annotations detected as being unique for 24- and 48-h treatment times, respectively. The polypeptides involved in protein metabolism, transport and signalling were annotated as the functional groups most strongly affected by 3-D clinorotation.

Download Full-text

Effects of an abscisic acid pretreatment on membrane leakage and lipid composition of Vigna unguiculata leaf discs subjected to osmotic stress

Plant Science ◽

10.1016/s0168-9452(97)00199-4 ◽

1997 ◽

Vol 130 (1) ◽

pp. 11-18 ◽

Cited By ~ 24

Author(s):

Paula Scotti Campos ◽

Anh Thu Pham Thi

Keyword(s):

Abscisic Acid ◽

Osmotic Stress ◽

Vigna Unguiculata ◽

Lipid Composition ◽

Acid Pretreatment ◽

Leaf Discs ◽

Membrane Leakage

Download Full-text

Response to abscisic acid application and hormone profiles in spring Brassica napus seed in relation to secondary dormancy

Canadian Journal of Botany ◽

10.1139/b04-119 ◽

2004 ◽

Vol 82 (11) ◽

pp. 1618-1624 ◽

Cited By ~ 15

Author(s):

Robert H Gulden ◽

Sheila Chiwocha ◽

Suzanne Abrams ◽

Ian McGregor ◽

Allison Kermode ◽

...

Keyword(s):

Abscisic Acid ◽

Brassica Napus ◽

Osmotic Stress ◽

Stress Sensitivity ◽

Brassica Napus L ◽

Secondary Dormancy ◽

Aba Sensitivity ◽

Treated Seed ◽

High Concentrations ◽

Osmotic Treatment

The plant hormone abscisic acid (ABA) has been implicated in the inception and maintenance of seed dormancy, while gibberellins promote dormancy breakage and germination in some species. We investigated whether osmotic stress induced secondary dormancy in Brassica napus L. is associated with changes in ABA sensitivity and metabolism, as well as changes in gibberellin levels. Seeds of two genotypes, one with low dormancy potential (LDP) and one with high dormancy potential (HDP) for secondary dormancy, were exposed to a dormancy-inducing osmotic treatment for up to 4 weeks and then germinated in the presence of increasing ABA concentrations. Even at relatively high concentrations of supplied ABA, germination of LDP seed was not inhibited, while relatively low ABA concentrations inhibited the germination of HDP seed after osmotic treatment. Fluridone was highly effective in suppressing secondary dormancy development in HDP seed, but had no effect on germinability in LDP seed. Despite the lack of differences in nonosmotically treated seed, ABA and ABA-glucose ester accumulated to higher levels, and gibberellin A1 accumulated to lower levels, in HDP relative to LDP seed by the end of the osmotic treatment. Our findings indicate an association among ABA sensitivity, biosynthesis and accumulation, and secondary dormancy potential in B. napus seed.Key words: abscisic acid (ABA), Brassica napus, fluridone, induced dormancy, osmotic stress, sensitivity.

Download Full-text