An in vitro model of allogeneic stimulation of cord blood

1999 ◽  
Vol 60 (7) ◽  
pp. 598-607 ◽  
Author(s):  
A El Ghalbzouri ◽  
B Drénou ◽  
V Blancheteau ◽  
C Choqueux ◽  
R Fauchet ◽  
...  
Keyword(s):  
Cord Blood ◽  
In Vitro Model ◽  
Vitro Model ◽  
Allogeneic Stimulation ◽  
Stimulation Of

scholarly journals Electrical Pulse Stimulation of Cultured Human Skeletal Muscle Cells as an In Vitro Model of Exercise

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33203 ◽  
Author(s):  
Nataša Nikolić ◽  
Siril Skaret Bakke ◽  
Eili Tranheim Kase ◽  
Ida Rudberg ◽  
Ingeborg Flo Halle ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Human Skeletal Muscle ◽  
In Vitro Model ◽  
Muscle Cells ◽  
Electrical Pulse Stimulation ◽  
Human Skeletal Muscle Cells ◽  
Vitro Model ◽  
Pulse Stimulation ◽  
Stimulation Of

THE EFFECT OF COD LIVER OIL INTAKE ON THE STIMULATION OF BLOOD CELLS

1987 ◽  
Author(s):  
J B Hansen ◽  
J O Olsen ◽  
L Wilagård ◽  
B Østerud
Keyword(s):  
Platelet Aggregation ◽  
Young Women ◽  
Whole Blood ◽  
Blood Cells ◽  
In Vitro Model ◽  
Cod Liver Oil ◽  
Blood Samples ◽  
Vitro Model ◽  
Stimulation Of

In an in vitro model, stimulation of blood cells with a low concentration of lipopolysaccharides (LPS) revealed differences between women and men that possibly could be an explanation to why young women have less coronary heart disease than men (see abstract Hansen et al. “A model to--”).This model was also used to study the effect of intake of cod liver oil (CLO). 40 students (20 men and 20 women) were tested followed by an intake of 25 ml CLO daily for 2 months by 20 of the students.Heparinized blood samples were incubated with 2 ng LPS/ ml for 2 hours followed by isolation of plasma for thromboxane B2 and 6-keto-PG 1α quantitation.After the first 2 months period of CLO drinking we have the following results:The two months of CLO intake had no significant effect pn the thromboplastin induced synthesis in monocytes. In addition platelet aggregation was tested in a whole blood aggregometer using ADP addition to heparinized blood or collagen induced platelet aggregation in citrated whole blood. ADP aggregation was reduced from 75.9 ± 16.8% to 55.4 ± 19% in the CLO group of women, whereas the reduction in the CLO group of men was 70.1 ± 17.1% to 60.9±18.6%. Similar result were found with collagen aggregation (57% to 33% for women and 48% to 30% for men).It is concluded that CLO intake reduces TxA2 production and plateletaggregation without having reduced effect on PGI2 production in whole blood.

scholarly journals Biological activity of interferon gamma and lipopolysaccharide on the nitric oxide production in C6 astroglioma cells and some unexpected effects of potentization

2021 ◽  
Vol 14 (2) ◽  
pp. 23-27
Author(s):  
Clara Bonafini ◽  
Marta Marzotto ◽  
Debora Olioso ◽  
Paolo Bellavite
Keyword(s):  
Nitric Oxide ◽  
Interferon Gamma ◽  
In Vitro Model ◽  
Oxygen Free Radicals ◽  
Cell Model ◽  
Vitro Model ◽  
Oxide Synthase ◽  
Stimulation Of ◽  
Astroglioma Cells

Background: A proinflammatory environment is a hallmark of several neurodegenerative diseases where astrocyte involvement is also well established. Astrocytes and microglia in central nervous system are mainly involved in the release of cytokines, oxygen free radicals and nitric oxide (NO). Several studies on C6 astroglioma cells, a widely used in vitro model for these events, demonstrated that co-stimulation of this cell line with bacterial lipopolysaccharide (LPS) and interferon gamma (IFN-) induces a synergistic nitric oxide synthase (iNOS) expression.1 In our laboratory we are using this versatile cell model in order to carefully investigate dose-response effects of various putative agonists or inhibitors and to assess the possible changes provoked in those agents by different procedures of dilution and succussion (agitation) (potentization according to the homeopathic terminology).

Stimulation of osteoblast-like cells with rh-TGF-ß, rh-FGF, rh-BMP 2 and rx-BMP 4 in an in-vitro model

2000 ◽  
Vol 29 (2) ◽  
pp. 0107-0111
Author(s):  
S. Kessler ◽  
S. Kastler ◽  
U. Mayr-Wohlfart ◽  
W. Puhl ◽  
K-P. Günther
Keyword(s):  
In Vitro Model ◽  
Vitro Model ◽  
Stimulation Of

Antigen stimulation of human pulmonary smooth muscle: An in vitro model of inflammation

1996 ◽  
Vol 12 (4-6) ◽  
pp. 239-244 ◽  
Author(s):  
I. Gorenne ◽  
C. Labat ◽  
J. P. Gascard ◽  
C. Brink
Keyword(s):  
Smooth Muscle ◽  
In Vitro Model ◽  
Antigen Stimulation ◽  
Vitro Model ◽  
Stimulation Of

An in vitro model to investigate the graft versus leukaemia effect of cord blood

1997 ◽  
Vol 56 ◽  
pp. 366
Author(s):  
J.A. Ademokun ◽  
J.A.E. Irving ◽  
J. Dunn ◽  
S.J. Proctor ◽  
A.M. Dickinson
Keyword(s):  
Cord Blood ◽  
In Vitro Model ◽  
Vitro Model
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