New geochemical and 40Ar/39Ar hornblende and biotite data from the Grenvillian Trenton Prong inlier provide the first constraints for the identification of lithotectonic units, their tectonic setting, and their metamorphic to post-metamorphic history. Gneissic tonalite, diorite, and gabbro compose the Colonial Lake Suite magmatic arc that developed along eastern Laurentia prior to 1.2 Ga. Spatially associated low- and high-TiO2 amphibolites were formed from island-arc basalt proximal to the arc front and mid-ocean ridge basalt-like basalt in a back-arc setting, respectively. Supracrustal paragneisses include meta-arkose derived from a continental sediment source of Laurentian affinity and metagraywacke and metapelite from an arc-like sediment source deposited in a back-arc basin, inboard of the Colonial Lake arc. The Assunpink Creek Granite was emplaced post-tectonically as small bodies of peraluminous syenogranite produced through partial melting of a subduction-modified felsic crustal source. Prograde mineral assemblages reached granulite- to amphibolite-facies metamorphic conditions during the Ottawan phase of the Grenvillian Orogeny. Hornblende 40Ar/39Ar ages of 935–923 Ma and a biotite age of 868 Ma record slow cooling in the northern part of the inlier following the metamorphic peak. Elsewhere in the inlier, biotite 40Ar/39Ar ages of 440 Ma and 377–341 Ma record partial to complete thermal resetting or new growth during the Taconian and Acadian orogens. The results of this study are consistent with the Trenton Prong being the down-dropped continuation of the Grenvillian New Jersey Highlands on the hanging wall of a major detachment fault. The Trenton Prong therefore correlates to other central and northern Appalachian Grenvillian inliers and to parts of the Grenville Province proper.