Double parasitisme a strongyloides stercoralis et giardia intestinalis chez une patiente infectée par HTLV-1

Introduction Intestinal parasitic diseases are a global health problem. Due to its equatorial climate, vast territory with isolated areas and the precariousness of its population, intestinal parasitosis is considered to be a major issue in French Guiana but only few data are available and these mainly focus on specific population. We aimed at determining the parasitic index and at describing the characteristics of these infections in order to develop preventive strategies. Material and methods We retrospectively analysed all the parasitological samples recorded in the register of the two main laboratories of French Guiana between 2011 and 2016. The parasitic index was the percentage of parasitised patients in comparison with the total number of subjects studied. A patient who underwent several positive parasitological examinations was considered only once in the analysis at the time of the first sampling. Results A total of 15,220 parasitological samples of 9,555 patients were analysed and 2,916 were positive in 1,521 patients. The average infestation rate and parasitic index were 19.2% and 16.0%, respectively. The parasitic index remained stable between 2011 (18.2%) and 2016 (18.3%). The patients were mainly men (66.4%), with a median age of 33.0 years (26.3% of patients were under 18 years of age) and lived mainly in the Central Agglomeration (48.2%) and in West Guiana (37.4%). Hookworms were the most common parasite (25.2%) followed by Entamoeba coli (13.3%), Strongyloides stercoralis (10.9%) and Giardia intestinalis (10.8%). Among the infected patients, 31.0% presented mixed infections and 67.5% of them had at least one pathogenic parasite. The patients aged from 0 to 18 years presented significantly more polyparasitism (30.9%) than monoparasitism (24.3%, p<0.001). Ancylostoma sp and Strongyloides stercoralis were mainly diagnosed during the rainy season (59.5% and 64.7% respectively), in men (78.6% and 81.1% respectively) and in patients aged from 18 to 65 years (86.6% and 76.6% respectively) whereas, Giardia intestinalis infected mostly children under 5 years (59.5%) of age. Conclusion Although it may not be representative of the entire Guyanese population, the parasitic index remained high and stable from 2011 and 2016 and it justifies the need for an active prevention program as it was already done in the other French overseas departments such as Martinique and Guadeloupe.

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Urban versus rural prevalence of intestinal parasites using multi-parallel qPCR in Colombia

10.1101/2020.09.20.20197616 ◽

2020 ◽

Author(s):

Patricia E Bryan ◽

Marcela Romero ◽

Miryan Sanchez ◽

Giovanny Torres ◽

Wilber Gomez ◽

...

Keyword(s):

Ascaris Lumbricoides ◽

Rural Areas ◽

Intestinal Parasites ◽

Parasite Burden ◽

Strongyloides Stercoralis ◽

Urban Slum ◽

Trichuris Trichiura ◽

Giardia Intestinalis ◽

Epidemiologic Surveillance ◽

Blastocystis Spp

Stool samples from 122 children from urban slum (n = 72) and rural (n = 50) areas were analyzed using multi-parallel real-time qPCR to determine the prevalence of intestinal parasites from two communities in Colombia. Findings indicated a prevalence of 86.1% Blastocystis spp., 62.5% Giardia intestinalis, 19.4% Cryptosporidium spp., 19.4% Ascaris lumbricoides, and 5.6% Trichuris trichiura in an urban slum; and 76% Blastocystis spp., 68% Giardia intestinalis, 20% Entamoeba histolytica, 50% Ascaris lumbricoides, 46% Trichuris trichiura and 2% Strongyloides stercoralis in rural areas. Polyparasitism was higher in rural (58%) compared to urban (25%) areas (p = 0.001). Trichuris trichiura burden was higher in the rural area (p = 0.002). Over 40% of helminth infections in rural areas had a heavy parasite burden by WHO classification. Over half of urban and rural children were infected with Giardia intestinalis and Blastocystis spp. Our data provides accurate epidemiologic surveillance for public health interventions.

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Estandarización de una reacción en cadena de la polimerasa en tiempo real (qPCR) para la detección de Strongyloides stercoralis en muestras de materia fecal

Medicina y Laboratorio ◽

10.36384/01232576.94 ◽

2016 ◽

Vol 22 (9-10) ◽

pp. 459-478

Author(s):

Laura F. Campo-Polanco ◽

José M. Hernández-Sarmiento ◽

Luz E. Botero-Palacio ◽

Lina A. Gutiérrez-Builes

Keyword(s):

Entamoeba Histolytica ◽

Ascaris Lumbricoides ◽

Strongyloides Stercoralis ◽

Trichuris Trichiura ◽

Giardia Intestinalis ◽

Hymenolepis Nana ◽

Entamoeba Dispar

Introducción: el diagnóstico de estrongiloidiasis se realiza de rutina en los laboratorios clínicos; sin embargo, su detección se dificulta debido a la baja excreción parasitaria y la baja sensibilidad de las pruebas parasitológicas empleadas. Objetivo: diseñar y estandarizar una PCR en tiempo real (qPCR) para la detección de ADN de Strongyloides stercoralis en muestras de materia fecal. Materiales y métodos: se establecieron las condiciones de qPCR y se evaluaron: a) la especificidad analítica mediante análisis BLASTn de secuencias obtenidas de muestras positivas para Strongyloides stercoralis, b) sensibilidad analítica mediante diluciones seriadas de muestras que contenían larvas de Strongyloides stercoralis y c) la ocurrencia de reacciones cruzadas con otros parásitos e inhibidores de la amplificación. Resultados: se amplificó un fragmento de 101 pb del gen 18S del ARN ribosomal. El valor de Ct osciló entre 23 y 29, tomando un Ct ≤35 como el punto de corte para muestras positivas. El análisis BLASTn de las secuencias obtenidas mostró un porcentaje de identidad del 98% con secuencias 18S del ARN ribosomal de Strongyloides stercoralis reportadas en la NCBI. El límite inferior de detección de la qPCR fue 0,9 ng/µL. No se evidenció reacción cruzada con Ascaris lumbricoides, Trichuris trichiura, Uncinarias, Hymenolepis nana, Entamoeba histolytica/Entamoeba dispar, Entamoeba hartmanni, Giardia intestinalis e Iodamoeba bütschlii. No se detectaron inhibidores en las muestras de materia fecal. Conclusiones: la sensibilidad y la especificidad analítica de la qPCR comparado con el examen directo de heces son del 100%; sin embargo, aún no es posible interpretar su utilidad clínica.

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Prevalência de enteroparasitoses na população do município de Maria Helena, Paraná

Ciência & Saúde Coletiva ◽

10.1590/s1413-81232010000300033 ◽

2010 ◽

Vol 15 (3) ◽

pp. 899-905 ◽

Cited By ~ 15

Author(s):

Simone Aparecida dos Santos ◽

Luiz Sérgio Merlini

Keyword(s):

Ascaris Lumbricoides ◽

Strongyloides Stercoralis ◽

Giardia Intestinalis

O estudo epidemiológico dos parasitas intestinais tem por objetivo determinar as principais doenças e seus respectivos agentes etiológicos que se encontram distribuídos por todo o mundo, de forma endêmica ou epidêmica. O objetivo desta pesquisa foi avaliar a prevalência de infecção por enteroparasitoses, em qual idade há sua maior prevalência, relacionando a infecção com a idade, o sexo e a região em que a pessoa vive, no município de Maria Helen (PR). A coleta de dados foi realizada de junho de 2004 a maio de 2006. Foram analisadas 431 amostras de fezes pelo método de sedimentação espontânea e centrífugo-flutuação. A prevalência de enteroparasitoses foi de 16%, sendo os enteroparasitas mais frequentes: Endolimax nana (6,5%), Entamoeba coli (3,5%), Giardia intestinalis (6,3%), Ascaris lumbricoides (1,4%), Strongyloides stercoralis (0,7%), Entrobius vermicularis (0,7%), Ancilostomídeo (0,2%), Entamoeba hystolitica e Taenia sp (0,2%). O poliparasitismo foi detectado em 3,2% das amostras. A faixa etária que apresentou maior prevalência foi de zero a nove anos, porém não houve diferença estatística entre os fatores analisados, uma vez que p>0,05. Diante desses resultados, conclui-se que o município apresenta características similares em relação à zona rural e urbana.

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Ascaris lumbricoides infection in pre-school children from Chiriqui Province, Panama

Parasitology ◽

10.1017/s0031182000058030 ◽

1987 ◽

Vol 95 (3) ◽

pp. 615-622 ◽

Cited By ~ 11

Author(s):

Celia V. Holland ◽

D. W. T. Crompton ◽

D. L. Taren ◽

M. C. Nesheim ◽

Diva Sanjur ◽

...

Keyword(s):

Data Analysis ◽

Rural Community ◽

Ascaris Lumbricoides ◽

Worm Burden ◽

Egg Production ◽

Strongyloides Stercoralis ◽

Epidemiological Survey ◽

Giardia Intestinalis ◽

Parasitic Infections ◽

Intestinal Parasitic Infections

SUMMARYAn epidemiological survey of intestinal parasitic infections was conducted in a sample of 203 children aged 3–5 years from a semi-urban and a rural community in Chiriqui, Panama, in 1983–4. On the basis of stool examinations, the prevalences of Ascaris lumbricoides, Trichuris trichiura, hookworm, Giardia intestinalis, Entamoeba spp. and Strongyloides stercoralis were found to be 27, 34, 14, 15, 5 and 4% respectively. The results from children from the two communities were compared. Polyparasitism occurred significantly more often in rural than semi-urban children. Following anthelmintic treatment with levamisole, the numbers of A. lumbricoides passed/child were recorded and the frequency distribution of the parasite was observed to be highly aggregated with a variance to mean ratio of 10·2. For A. lumbricoides, relationships between worm burden, worm biomass and egg production were investigated. In the data analysis, an attempt was made to explore the influence of numbers of male worms on egg production. The results are compared with those obtained during other recent studies on the epidemiology of A. lumbricoides infection in other countries.

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PREVALENCIA DEL PARASITISMO INTESTINAL EN MANIPULADORES DE ALIMENTOS ATENDIDOS EN LA MUNICIPALIDAD DE LIMA METROPOLITANA, PERÚ

Neotropical Helminthology ◽

10.24039/rnh2012621015 ◽

2021 ◽

Vol 6 (2) ◽

Author(s):

Wignard Villegas ◽

José Iannacone ◽

Elsa Oré ◽

Luz Bazán

Keyword(s):

Strongyloides Stercoralis ◽

Trichuris Trichiura ◽

Giardia Intestinalis ◽

Enterobius Vermicularis ◽

Hymenolepis Nana ◽

Blastocystis Hominis ◽

Necator Americanus ◽

Ancylostoma Duodenale

El objetivo del presente trabajo fue determinar la prevalencia del parasitismo intestinal en manipuladores de alimentos atendidos en la Municipalidad de Lima Metropolitana, Perú. Entre julio y agosto del 2011 se examinaron 217 muestras de heces. Se emplearon los métodos Directo con Lugol, Técnica de Ritchie o centrifugación con formol-acetato de etilo como método de concentración y la coloración de Ziehl-Neelsen modificada para la detección de coccidios. Del total de muestras, 107 (49,3%) eran del sexo masculino y 110 (50,7%) del sexo femenino. La edad promedio de la población estudiada fue de 26,78 años. La prevalencia total del parasitismo intestinal fue de 71,89%, no encontrándose diferencias estadísticamente significativas en la prevalencia del parasitismo en cuanto al género y edad de la población estudiada. La mayor prevalencia del parasitismo intestinal se encontró en el grupo de edad de 18 a 27 años (46,1%). Las especies de protozoos más prevalentes fueron: Blastocystis hominis (55,8%), Entamoeba coli (30,9%), Endolimax nana (20,7%), Chilomastix mesnili (5,5%) y Giardia intestinalis (5,1%). Los helmintos encontrados fueron: Hymenolepis nana (3,7%), Necator americanus/Ancylostoma duodenale (2,3%), Strongyloides stercoralis (1,8%), Trichuris trichiura (1,4%), Diphyllobothrium sp. (0,5%), Enterobius vermicularis (0,5%) y Trichostrongylus sp. (0,5%). Predominó el monoparasitismo (33,64%) y en las asociaciones parasitarias fue más frecuente el biparasitismo (21,2%). El índice de diversidad beta de similaridad de Kulczynski-1 mostró que los protozoos están más asociados entre sí y de igual manera los helmintos entre sí. La técnica de Ritchie mostró mayor eficiencia que el método directo en el diagnóstico de quistes de protozoos.

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