Celastrus paniculatus seed water soluble extracts protect cultured rat forebrain neuronal cells from hydrogen peroxide-induced oxidative injury

Recently, research and development in the field of drug delivery systems (DDS) facilitating site-specific therapy has reached significant progression. DDS based on polymer micelles, coated micro- and nanoparticles, and various prodrug systems including water-soluble polymer have been prepared and extensively studied as novel drugs designed for cancer chemotherapy and brain delivery. Since polymers are going to be used in human, this study has the interest of testing two types of polymer, polyimides (PI) and polyphenylenevinylene (PPV) on neuronal cells. The objective of this study was to determine the possible neurotoxicity and potential neuroprotective effects of PI and PPV towards SH-SY5Y neuronal cells challenged by hydrogen peroxide (H2O2) as an oxidant. Cells were pretreated with either PI or PPV for 1 hour followed by incubation for 24 hour with 100 µM of H2O2. MTS assay was used to assess cell viability. Results show that PI and PPV are not harmful within the concentration up to 10 µM and 100 µM, respectively. However, PI and PPV do not protect neuronal cells against toxicity induced by H2O2 or further up the cell death.

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Celastrus paniculatus seed water soluble extracts protect against glutamate toxicity in neuronal cultures from rat forebrain

Journal of Ethnopharmacology ◽

10.1016/j.jep.2004.03.051 ◽

2004 ◽

Vol 93 (2-3) ◽

pp. 213-219 ◽

Cited By ~ 16

Author(s):

Praful B Godkar ◽

Richard K Gordon ◽

Arippa Ravindran ◽

Bhupendra P Doctor

Keyword(s):

Water Soluble ◽

Glutamate Toxicity ◽

Neuronal Cultures ◽

Rat Forebrain ◽

Celastrus Paniculatus

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A novel Keap1 inhibitor iKeap1 activates Nrf2 signaling and ameliorates hydrogen peroxide-induced oxidative injury and apoptosis in osteoblasts

Cell Death and Disease ◽

10.1038/s41419-021-03962-8 ◽

2021 ◽

Vol 12 (7) ◽

Author(s):

Yue-huan Zheng ◽

Jian-jun Yang ◽

Pei-jun Tang ◽

Yuan Zhu ◽

Zhe Chen ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Nuclear Translocation ◽

Oxidative Injury ◽

Potential Effect ◽

Protein Stabilization ◽

Human Osteoblasts ◽

Nrf2 Knockout ◽

Anti Oxidant ◽

Species Production ◽

Nrf2 Protein

AbstractAn ultra-large structure-based virtual screening has discovered iKeap1 as a direct Keap1 inhibitor that can efficiently activate Nrf2 signaling. We here tested its potential effect against hydrogen peroxide (H2O2)-induced oxidative injury in osteoblasts. In primary murine and human osteoblasts, iKeap1 robustly activated Nrf2 signaling at micromole concentrations. iKeap1 disrupted Keap1-Nrf2 association, causing Nrf2 protein stabilization, cytosol accumulation and nuclear translocation in murine and human osteoblasts. The anti-oxidant response elements (ARE) activity and transcription of Nrf2-ARE-dependent genes (including HO1, NQO1 and GCLC) were increased as well. Significantly, iKeap1 pretreatment largely ameliorated H2O2-induced reactive oxygen species production, lipid peroxidation and DNA damage as well as cell apoptosis and programmed necrosis in osteoblasts. Moreover, dexamethasone- and nicotine-induced oxidative injury and apoptosis were alleviated by iKeap1. Importantly, Nrf2 shRNA or CRISPR/Cas9-induced Nrf2 knockout completely abolished iKeap1-induced osteoblast cytoprotection against H2O2. Conversely, CRISPR/Cas9-induced Keap1 knockout induced Nrf2 cascade activation and mimicked iKeap1-induced cytoprotective actions in murine osteoblasts. iKeap1 was ineffective against H2O2 in the Keap1-knockout murine osteoblasts. Collectively, iKeap1 activated Nrf2 signaling cascade to inhibit H2O2-induced oxidative injury and death of osteoblasts.

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Hydrogen Peroxide Toxicity Induces Ras Signaling in Human Neuroblastoma SH-SY5Y Cultured Cells

Journal of Biomedicine and Biotechnology ◽

10.1155/2010/803815 ◽

2010 ◽

Vol 2010 ◽

pp. 1-4 ◽

Cited By ~ 7

Author(s):

Jirapa Chetsawang ◽

Piyarat Govitrapong ◽

Banthit Chetsawang

Keyword(s):

Hydrogen Peroxide ◽

Cell Viability ◽

Cultured Cells ◽

Neuronal Cells ◽

Competitive Inhibitor ◽

Ras Signaling ◽

Ras Proteins ◽

Human Neuroblastoma ◽

Dependent Signaling Pathway

It has been reported that overproduction of reactive oxygen species occurs after brain injury and mediates neuronal cells degeneration. In the present study, we examined the role of Ras signaling on hydrogen peroxide-induced neuronal cells degeneration in dopaminergic neuroblastoma SH-SY5Y cells. Hydrogen peroxide significantly reduced cell viability in SH-SY5Y cultured cells. An inhibitor of the enzyme that catalyzes the farnesylation of Ras proteins, FTI-277, and a competitive inhibitor of GTP-binding proteins, GDP-beta-S significantly decreased hydrogen peroxide-induced reduction in cell viability in SH-SY5Y cultured cells. The results of this study might indicate that a Ras-dependent signaling pathway plays a role in hydrogen peroxide-induced toxicity in neuronal cells.

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Decoloration of Azo Dyes by Hydrogen Peroxide Catalyzed by Water-Soluble Manganese Porphyrins

Textile Research Journal ◽

10.1177/004051759906901212 ◽

1999 ◽

Vol 69 (12) ◽

pp. 956-960 ◽

Cited By ~ 18

Author(s):

J. Tokuda ◽

R. Ohura ◽

T. Iwasaki ◽

Y. Takeuchi ◽

A. Kashiwada ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Azo Dyes ◽

Water Soluble ◽

Manganese Porphyrins

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Characterizing Wool Keratin

Research Letters in Materials Science ◽

10.1155/2009/147175 ◽

2009 ◽

Vol 2009 ◽

pp. 1-5 ◽

Cited By ~ 20

Author(s):

Jeanette M. Cardamone ◽

Alberto Nuñez ◽

Rafael A. Garcia ◽

Mila Aldema-Ramos

Keyword(s):

Hydrogen Peroxide ◽

Gel Electrophoresis ◽

Water Soluble ◽

Alkaline Solutions ◽

Biodegradable Material ◽

Alkaline Hydrogen Peroxide ◽

Cortical Cells ◽

Sds Page ◽

Fibrous Matrices ◽

Wool Keratin

Keratin from wool is a reactive, biocompatible, and biodegradable material. As the biological structural component of skin (soft keratins) and of nails, claws, hair, horn, feathers, and scales (hard keratins) pure keratin comprises up to 90% by weight of wool. Wool was treated in alkaline solutions to extract from 68% to 82% keratin within 2 to 5 hours of exposure at . The keratin products were water-soluble and were confirmed to contain intermediate filament and microfibrillar component-proteins of fractured, residual cuticle, and cortical cells. Oxidation of wool by peroxycarboximidic acid in alkaline hydrogen peroxide produced keratin products with distinct microcrystalline structures: descaled fibers, fibrous matrices, and lyophilized powders. Morphology and confirmation of peptide functionality were documented by SEM, Amino Acid Analysis, SDS-PAGE gel electrophoresis, MALDI-TOF/TOF, and FTIR analyses. The reactivity of keratin from wool models the reactivity of keratin from low-value sources such as cattle hair.

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Unusual kinetic role of a water-soluble iron(III) porphyrin catalyst in the oxidation of 2,4,6-trichlorophenol by hydrogen peroxide

International Journal of Chemical Kinetics ◽

10.1002/kin.20018 ◽

2004 ◽

Vol 36 (8) ◽

pp. 449-455 ◽

Cited By ~ 13

Author(s):

Gábor Lente ◽

James H. Espenson

Keyword(s):

Hydrogen Peroxide ◽

Water Soluble ◽

Soluble Iron

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Formation of Water-Soluble Fullerenes [C60,C70] under Ultrasonication and Antioxidant Effect

Eurasian Chemico-Technological Journal ◽

10.18321/ectj591 ◽

2017 ◽

Vol 5 (1) ◽

pp. 61

Author(s):

Weon-Bae Ko ◽

Hong-Seok Jeong ◽

Sung-Ho Hwang

Keyword(s):

Hydrogen Peroxide ◽

Room Temperature ◽

Antioxidant Effect ◽

Water Soluble ◽

Maldi Tof Ms ◽

Pc 12 Cells ◽

Effect Of Water ◽

Maldi Tof ◽

Line Following ◽

Tof Ms

The water-soluble fullerenes [C60, C70] are prepared with fullerenes [C60, C70] and a mixture of oxidants (v/v) at the ratio of 3:1 under ultrasonic condition at room temperature. The MALDI-TOF MS confirmed that the water-soluble compounds were C60 and C70. The antioxidant effect of water-soluble fullerenes [C60, C70] in the PC 12 cells (Rat pheochromocytoma) line following exposure to hydrogen peroxide (H2O2) was investigated.

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