Isolation and functional characterization of a temperature-sensitive mutant of the yeast Saccharomyces cerevisiae in translation initiation factor eIF5: an eIF5-dependent cell-free translation system

Gene ◽  
2000 ◽  
Vol 244 (1-2) ◽  
pp. 109-118 ◽  
Author(s):  
Tapan Maiti ◽  
Supratik Das ◽  
Umadas Maitra
Keyword(s):  
Functional Characterization ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Translation System ◽  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Yeast Saccharomyces Cerevisiae ◽  
Free Translation ◽  
Dependent Cell

Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases

1982 ◽  
Vol 152 (1) ◽  
pp. 357-362
Author(s):  
D Elseviers ◽  
P Gallagher ◽  
A Hoffman ◽  
B Weinberg ◽  
I Schwartz
Keyword(s):  
Trna Synthetase ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Translation System ◽  
Temperature Sensitive ◽  
Regulation Of Expression ◽  
Trna Synthetases ◽  
E Coli ◽  
Translation Initiation Factor 3

A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1-transformed NP37 overproduce initiation factor 3 and phenylalanyl-tRNA synthetase. Gene expression from pID1 was studied in vitro in a coupled transcription-translation system and in minicells. The results suggest that the genes for initiation factor 3 and phenylalanyl- and threonyl-tRNA synthetase are regulated by different mechanisms.

Functional characterization of the translation initiation factor eIF4E of Echinococcus granulosus

2019 ◽  
Vol 118 (10) ◽  
pp. 2843-2855
Author(s):  
Filipe Santos Pereira-Dutra ◽  
Martin Cancela ◽  
Bruna Valandro Meneghetti ◽  
Henrique Bunselmeyer Ferreira ◽  
Karina Mariante Monteiro ◽  
...  
Keyword(s):  
Echinococcus Granulosus ◽  
Translation Initiation ◽  
Functional Characterization ◽  
Translation Initiation Factor ◽  
Initiation Factor

Translation in Saccharomyces cerevisiae: initiation factor 4E-dependent cell-free system

1989 ◽  
Vol 9 (10) ◽  
pp. 4467-4472
Author(s):  
M Altmann ◽  
N Sonenberg ◽  
H Trachsel
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Point Mutations ◽  
Translation Initiation Factor ◽  
Apparent Temperature ◽  
Initiation Factor ◽  
Temperature Sensitive ◽  
Wild Type ◽  
Free System ◽  
Gal1 Promoter

The gene encoding translation initiation factor 4E (eIF-4E) from Saccharomyces cerevisiae was randomly mutagenized in vitro. The mutagenized gene was reintroduced on a plasmid into S. cerevisiae cells having their only wild-type eIF-4E gene on a plasmid under the control of the regulatable GAL1 promoter. Transcription from the GAL1 promoter (and consequently the production of wild-type eIF-4E) was then shut off by plating these cells on glucose-containing medium. Under these conditions, the phenotype conferred upon the cells by the mutated eIF-4E gene became apparent. Temperature-sensitive S. cerevisiae strains were identified by replica plating. The properties of one strain, 4-2, were further analyzed. Strain 4-2 has two point mutations in the eIF-4E gene. Upon incubation at 37 degrees C, incorporation of [35S]methionine was reduced to 15% of the wild-type level. Cell-free translation systems derived from strain 4-2 were dependent on exogenous eIF-4E for efficient translation of certain mRNAs, and this dependence was enhanced by preincubation of the extract at 37 degrees C. Not all mRNAs tested required exogenous eIF-4E for translation.

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