Characterization of Wine Yeast Strains of the Saccharomyces Genus on the Basis of Molecular Markers: Relationships Between Genetic Distance and Geographic or Ecological Origin

1996 ◽  
Vol 19 (1) ◽  
pp. 122-132 ◽  
Author(s):  
José Manuel Guillamón ◽  
Eladio Barrio ◽  
Amparo Querol
Keyword(s):  
Molecular Markers ◽  
Genetic Distance ◽  
Wine Yeast ◽  
Yeast Strains

scholarly journals Caracterización molecular de una población de ganado Caqueteño y su relación filogenética con razas bovinas criollas colombianas

2006 ◽  
Vol 7 (1) ◽  
pp. 33 ◽  
Author(s):  
Gloria Patricia Barrera ◽  
Rodrigo Martínez ◽  
Rafael Torrijos ◽  
Francisco Ramón
Keyword(s):  
Molecular Markers ◽  
Genetic Distance ◽  
Bos Indicus ◽  
Racial Group ◽  
The Other ◽  
Fixation Index ◽  
Blood Samples ◽  
Creole Cattle ◽  
Zebu Breed

<p>El objetivo fue determinar si una población de la raza bovina criolla Caqueteño (CAQ) representa una agrupación racial diferente de las demás poblaciones criollas bovinas colombianas. A tal fin se realizó extracción de ADN a partir de muestras de sangre tomadas a 80 animales Caqueteño; además, se usaron 126 muestras de ADN pertenecientes a seis razas criollas colombianas, 19 muestras de ADN tomadas de animales Cebú (<em>Bos indicus</em>) y 14 de una raza española autóctona. Para caracterizar la población se utilizaron 14 marcadores moleculares tipo microsatélite para los cuales se identificaron los genotipos mediante PCR y electroforesis. En la población CAQ se encontró un número promedio de alelos (NPA= 9,21) superior a las demás razas evaluadas, que variaron entre NPA= 4,57 y NPA= 8,57 para las razas Sanmartinero y BON, respectivamente. La endogamia, definida mediante el índice de fijación (Fis), fue similar a otras razas criollas (0,14) y se encuentra dentro de los parámetros reportados en otros trabajos. Los menores valores de distancias genéticas fueron encontrados entre la población CAQ y las razas BON (0,15) y Romosinuano (0,17) y se constató una distancia considerable con la raza Cebú (0,27). En general, los análisis genotípicos muestran que el biotipo CAQ presenta uniformidad racial con introgresión moderada de la raza cebú, por lo que puede considerarse como un grupo racial definido que puede ser utilizado para un programa de conservación.</p><p> </p><p><strong>The molecular characterization of a population of Caqueteño cattle and their phylogenetic relationship with Colombian creole cattle breeds</strong></p><p>The object of this work was to determine whether a population of Caqueteño creole cattle (CAC) represented a racial grouping different to other Colombian creole populations. DNA was thus extracted from blood-samples taken from 80 CAC animals; 126 samples of DNA from six Colombian creole breeds, 19 DNA samples from zebu animals (Bos indicus) and 14 from an autochthonous Spanish breed were also used. 14 micro-satellite molecular markers were used for characterising the population, their genotypes being identified by PCR and electrophoresis. A greater average number of alleles (ana=9.21) was found in the CAC population than in the other breeds being evaluated, varying from ana=4.57 to ana=8.57 for Sanmartinero and BON breeds, respectively. Endogamy, defined by fixation index (Fis), was similar to other creole breeds (0.14), coming within parameters reported in other paper. The smallest genetic distance values were found between the CAC population and BON (0.15) and Romosinuano (0.17) breeds, though a considerable distance was found from the zebu breed (0.27). Genotyping analysis generally revealed that the CAC biotype presented racial uniformity, the zebu breed having moderate introgression, meaning that it could be considered to be a well defined racial group which could be used for a conservation program.</p>

Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple

2018 ◽  
Vol 39 (4) ◽  
pp. 474-482
Author(s):  
Hoang Thi Le Thuong ◽  
Nguyen Quang Hao ◽  
Tran Thi Thuy
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Low Ph ◽  
Yeast Strains ◽  
Biological Studies ◽  
Taxonomic Characterization

Eight yeast strains (denoted as D1 to D8) were isolated from samples of natural fermented pineapple. Strain D8 showed highest alcoholic production at low pH and special aroma of pineapple has been chosen for further study. Taxonomic characterization of strain D8 using morphological, biochemical and molecular biological studies confirmed that strain D8  belong to Saccharomycetaceae family, Saccharomycetales order and Saccharomyces cerevisiae species. Therefore, we named this strain as Saccharomyces cerevisiae D8 for further study on Brandy production from pineapple. Citation: Hoang Thi Le Thuong, Nguyen Quang Hao, Tran Thi Thuy, 2017. Taxonomic characterization and identification of Saccharomyces cerevisiae D8 for brandy production from pineapple. Tap chi Sinh hoc, 39(4): 474- 482. DOI: 10.15625/0866-7160/v39n4.10864.*Corresponding author: [email protected] Received 5 December 2016, accepted 12 August 2017

Evaluation of Molecular Markers Specific for 1BL∙1RS Translocation and Characterization of 1RS Chromosome in Wheat Varieties from Different Origins

2009 ◽  
Vol 35 (11) ◽  
pp. 2107-2115 ◽  
Author(s):  
Huai-Jun TANG ◽  
Gui-Hong YIN ◽  
Xian-Chun XIA ◽  
Jian-Jun FENG ◽  
Yan-Ying QU ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Wheat Varieties ◽  
Different Origins

scholarly journals Characterization of genome-reduced fission yeast strains

2013 ◽  
Vol 41 (10) ◽  
pp. 5382-5399 ◽  
Author(s):  
Mayumi Sasaki ◽  
Hiromichi Kumagai ◽  
Kaoru Takegawa ◽  
Hideki Tohda
Keyword(s):  
Fission Yeast ◽  
Yeast Strains

Characterization of High-Copy-Number Retrotransposons From the Large Genomes of the Louisiana Iris Species and Their Use as Molecular Markers

Genetics ◽  
2003 ◽  
Vol 164 (2) ◽  
pp. 685-697 ◽  
Author(s):  
Edward K Kentner ◽  
Michael L Arnold ◽  
Susan R Wessler
Keyword(s):  
Molecular Markers ◽  
Copy Number ◽  
High Copy Number ◽  
Marker System ◽  
Transposon Display ◽  
Plant Genomes ◽  
Large Plant ◽  
Backcross Hybrids ◽  
Abundance And Distribution

Abstract The Louisiana iris species Iris brevicaulis and I. fulva are morphologically and karyotypically distinct yet frequently hybridize in nature. A group of high-copy-number TY3/gypsy-like retrotransposons was characterized from these species and used to develop molecular markers that take advantage of the abundance and distribution of these elements in the large iris genome. The copy number of these IRRE elements (for iris retroelement), is ∼1 × 105, accounting for ∼6–10% of the ∼10,000-Mb haploid Louisiana iris genome. IRRE elements are transcriptionally active in I. brevicaulis and I. fulva and their F1 and backcross hybrids. The LTRs of the elements are more variable than the coding domains and can be used to define several distinct IRRE subfamilies. Transposon display or S-SAP markers specific to two of these subfamilies have been developed and are highly polymorphic among wild-collected individuals of each species. As IRRE elements are present in each of 11 iris species tested, the marker system has the potential to provide valuable comparative data on the dynamics of retrotransposition in large plant genomes.

scholarly journals The Fitness Advantage of Commercial Wine Yeasts in Relation to the Nitrogen Concentration, Temperature, and Ethanol Content under Microvinification Conditions

2013 ◽  
Vol 80 (2) ◽  
pp. 704-713 ◽  
Author(s):  
Estéfani García-Ríos ◽  
Alicia Gutiérrez ◽  
Zoel Salvadó ◽  
Francisco Noé Arroyo-López ◽  
José Manuel Guillamon
Keyword(s):  
Abiotic Factors ◽  
Nitrogen Concentration ◽  
Wine Yeast ◽  
Wine Industry ◽  
Wine Yeasts ◽  
Simple Method ◽  
Fitness Advantage ◽  
Yeast Strains ◽  
Industrial Strains ◽  
Nitrogen Concentrations

ABSTRACTThe effect of the main environmental factors governing wine fermentation on the fitness of industrial yeast strains has barely received attention. In this study, we used the concept of fitness advantage to measure how increasing nitrogen concentrations (0 to 200 mg N/liter), ethanol (0 to 20%), and temperature (4 to 45°C) affects competition among four commercial wine yeast strains (PDM, ARM, RVA, and TTA). We used a mathematical approach to model the hypothetical time needed for the control strain (PDM) to out-compete the other three strains in a theoretical mixed population. The theoretical values obtained were subsequently verified by competitive mixed fermentations in both synthetic and natural musts, which showed a good fit between the theoretical and experimental data. Specifically, the data show that the increase in nitrogen concentration and temperature values improved the fitness advantage of the PDM strain, whereas the presence of ethanol significantly reduced its competitiveness. However, the RVA strain proved to be the most competitive yeast for the three enological parameters assayed. The study of the fitness of these industrial strains is of paramount interest for the wine industry, which uses them as starters of their fermentations. Here, we propose a very simple method to model the fitness advantage, which allows the prediction of the competitiveness of one strain with respect to different abiotic factors.

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