Determination of the total charge in the cell walls of Gram-positive bacteria

1997 ◽  
Vol 9 (1-2) ◽  
pp. 81-100 ◽  
Author(s):  
Albert van der Wal ◽  
Willem Norde ◽  
Alexander J.B. Zehnder ◽  
Johannes Lyklema
Keyword(s):  
Cell Walls ◽  
Total Charge ◽  
Gram Positive ◽  
Gram Positive Bacteria

Chemical analysis of isolated cell walls of Gram-positive bacteria and determination of the cell wall to cell mass ratio

1997 ◽  
Vol 28 (2) ◽  
pp. 147-157 ◽  
Author(s):  
Albert van der Wal ◽  
Willem Norde ◽  
Bernd Bendinger ◽  
Alexander J.B Zehnder ◽  
Johannes Lyklema
Keyword(s):  
Cell Wall ◽  
Chemical Analysis ◽  
Cell Walls ◽  
Cell Mass ◽  
Mass Ratio ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Isolated Cell

Antimicrobial Activity of Isopteropodine

2005 ◽  
Vol 60 (5-6) ◽  
pp. 385-388 ◽  
Author(s):  
Rubén García ◽  
Cesia Cayunao ◽  
Ronny Bocic ◽  
Nadine Backhouse ◽  
Carla Delporte ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Gram Positive ◽  
Uncaria Tomentosa ◽  
Gram Positive Bacteria

Bioassay-directed fractionation for the determination of antimicrobial activity of Uncaria tomentosa, has led to the isolation of isopteropodine (0.3%), a known Uncaria pentacyclic oxindol alkaloid that exhibited antibacterial activity against Gram positive bacteria.

Antimicrobial Activity of Fractions and Compounds from Calophyllum brasiliense (Clusiaceae/Guttiferae)

2004 ◽  
Vol 59 (9-10) ◽  
pp. 657-662 ◽  
Author(s):  
Juliana B. Pretto ◽  
Valdir Cechinel-Filho ◽  
Vânia F. Noldin ◽  
Mara R. K. Sartori ◽  
Daniela E. B Isaias ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Pathogenic Bacteria ◽  
Protocatechuic Acid ◽  
Inhibitory Concentration ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Methanolic Extracts ◽  
Calophyllum Brasiliense

Abstract Calophyllum brasiliense (Clusiaceae/Guttiferae) is a native Brazilian medicinal plant traditionally used against several diseases, including infectious pathologies. Crude methanolic extracts (CME) and two fractions, denoted non-polar (soluble in chloroform) and polar (nonsoluble in chloroform), were prepared from different parts of the plant (roots, stems, leaves, flowers and fruits) and studied. The following compounds were isolated and tested against pathogenic bacteria and yeasts by determination of the minimal inhibitory concentration (MIC): brasiliensic acid (1), gallic acid (2), epicatechin (3), protocatechuic acid (4), friedelin (5) and 1,5-dihydroxyxanthone (6). The results indicated that all the parts of the plant exhibited antimicrobial activity against Gram-positive bacteria, which are selectively inhibited by components of C. brasiliense. No activity was observed against Gram-negative bacteria and yeasts tested. Regarding the isolated compounds, substance 4 showed antimicrobial activity against all the tested microorganisms, whereas compound 6 exhibited antimicrobial activity only against Gram-positive bacteria. The results from the current study confirm and justify the popular use of this plant to treat infectious processes.

scholarly journals Selective extraction of polymers from cell walls of Gram-positive bacteria (Short Communication)

1973 ◽  
Vol 131 (3) ◽  
pp. 619-621 ◽  
Author(s):  
Jiri G. Pavlik ◽  
Howard J. Rogers
Keyword(s):  
Bacillus Licheniformis ◽  
Cell Walls ◽  
Short Communication ◽  
Selective Extraction ◽  
Gram Positive ◽  
Teichoic Acids ◽  
Gram Positive Bacteria ◽  
Teichuronic Acid

Brief heating of Bacillus Licheniformis cell walls at 100°C in aqueous buffers of pH3.0–4.0 removes some polymers but not others from the mucopeptides. For example, relatively undegraded teichuronic acid can be extracted at 100°C in 20min at pH3.0 whereas the teichoic acids are not removed. Similar specificity can be shown with walls from three other species of micro-organism.

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