Activation of PKC, superoxide anion production and LDL lipid peroxidation are not dependent on phosphoinositide-specific phospholipase C activity in U937 cells

1997 ◽  
Vol 17 (3) ◽  
pp. 175-189 ◽  
Author(s):  
Qing Li ◽  
Martha K Cathcart
Keyword(s):  
Lipid Peroxidation ◽  
Phospholipase C ◽  
Superoxide Anion ◽  
U937 Cells ◽  
Superoxide Anion Production ◽  
Anion Production

scholarly journals Pardaxin Promoted Differentiation and Maturation of Leukemic Cells via Regulating TLR2/MyD88 Signal against Cell Proliferation

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Wu-Ching Uen ◽  
Chen-Yen Choong ◽  
Chen-Jei Tai ◽  
Cheng-Jeng Tai
Keyword(s):  
Blood Cells ◽  
Superoxide Anion ◽  
Therapeutic Potential ◽  
Leukemic Cells ◽  
U937 Cells ◽  
Toll Like Receptor ◽  
Marine Fish Species ◽  
Superoxide Anion Production ◽  
Anion Production ◽  
Myd88 Protein

Objective. Leukemia is a cancer of the blood cells. Leukemic THP-1 and U937 cells were used in this study as monocytic effectors cells for proliferation responses and macrophage-like cells induction in leukemia. Pardaxin is an antimicrobial peptide isolated from the marine fish species.Methods. After treatment for 5 days, pardaxin significantly suppressed cell viability and arrested cell cycle at G0/G1 phase in leukemic cells which were evaluated.Results. Pardaxin also induced cell differentiation and maturation of THP-1 and U937 cells into macrophage-like cells with phagocytotic ability. Moreover, pardaxin elevated the expression of MyD88 but not toll-like receptor (TLR)-2 in both leukemic cells. TLR-2 blocking peptide was used to confirm that pardaxin attenuated phagocytotic ability and superoxide anion production in leukemic cells via activating MyD88 protein.Conclusions. These findings suggested that pardaxin has a therapeutic potential for leukemia.

Lactate and P o 2 Modulate Superoxide Anion Production in Bovine Cardiac Myocytes

Circulation ◽  
1997 ◽  
Vol 96 (2) ◽  
pp. 614-620 ◽  
Author(s):  
Kamal M. Mohazzab-H. ◽  
Pawel M. Kaminski ◽  
Michael S. Wolin
Keyword(s):  
Cardiac Myocytes ◽  
Superoxide Anion ◽  
Superoxide Anion Production ◽  
Anion Production

scholarly journals Opsonization of Actinobacillus actinomycetemcomitans by Immunoglobulin G Antibody Reactive with Phosphorylcholine

2002 ◽  
Vol 70 (11) ◽  
pp. 6485-6488 ◽  
Author(s):  
Donald Purkall ◽  
John G. Tew ◽  
Harvey A. Schenkein
Keyword(s):  
Streptococcus Pneumoniae ◽  
Immunoglobulin G ◽  
Oxidative Burst ◽  
Superoxide Anion ◽  
Polymorphonuclear Neutrophils ◽  
Actinobacillus Actinomycetemcomitans ◽  
Superoxide Anion Production ◽  
Protective Antibody ◽  
Anion Production ◽  
Human Sera

ABSTRACT We used two strains of Actinobacillus actinomycetemcomitans, one bearing phosphorylcholine (PC) (strain D045D-40) and one devoid of PC antigens (strain DB03A-42), as well as a nonencapsulated strain of Streptococcus pneumoniae (strain 39937), to examine the opsonic properties of physiological concentrations (⩽30 μg/ml) of purified human anti-PC immunoglobulin G (IgG). Anti-PC bound to both A. actinomycetemcomitans DO45D-40 and S. pneumoniae 39937 and induced superoxide anion production by polymorphonuclear neutrophils; induction of the oxidative burst was inhibited by antibodies to either CD16 or CD32. Thus, anti-PC IgG at concentrations present in most human sera promotes the opsonization of PC-expressing strains of A. actinomycetemcomitans in the absence of complement, implying that anti-PC may be a protective antibody against such strains of bacteria.

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