Influence of oestrogens on formation of reactive oxygen species in liver microsomes of differently aged male Wistar rats

1999 ◽  
Vol 51 (4-5) ◽  
pp. 282-288 ◽  
Author(s):  
A. Barth ◽  
G. Landmann ◽  
K. Liepold ◽  
H. Zapf ◽  
D. Müller ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wistar Rats ◽  
Liver Microsomes ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Male Wistar Rats

scholarly journals Pengaruh madu terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) yang diberi paparan asap rokok

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Pretty P. Makasenda ◽  
Janette M. Rumbajan ◽  
Grace L.A. Turalaki
Keyword(s):  
Reactive Oxygen Species ◽  
Treatment Group ◽  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Significant Difference ◽  
Male Wistar Rats

Abstract: Cigarettes are composed of hazardous chemicals such as nicotine, tar and carbon monoxide (CO) to name a few. In just a single puff of a cigarette, there are 1014 free radical molecules also known as Reactive Oxygen Species (ROS) that can cause sperm damage. Various natural ingredients native to Indonesia were found to contain various antioxidants, one of them is honey. The effects of honey as antioxidant may protect body cells in neutralizing free radicals caused by smoking and reducing the damage to spermatozoa cell that is caused by ROS and thereby avoiding the declining quality of spermatozoa. This study aimed to determine the effect of honey on the quality of spermatozoa of Wistar rats (Rattus norvegicus) that had been exposed to cigarette smoke. Subjects of this study were nine male Wistar rats (Rattus norvegicus) randomly divided into three groups weighing 150-200 g with the age range of 12-14 weeks. Each group of mice was given exposure to the smoke of 2 cigarettes a day in which the treatment group (P1) is also given 0.5 ml of honey per day, and the treatment group (P2) honey 1 ml / day. The results showed that honey treatment can improve concentration, motility, and morphology of spermatozoa Wistar rats (Rattus norvegicus) by exposure to cigarrets smoke. Occurred a significant difference of concentration, motility, and morphology of spermatozoa group given only the exposure to cigarette smoke and the group given exposure to cigarette smoke and honey. This results showed that 1 ml of honey per day could improve the quality of spermatozoa.Keywords: honey, cigarrets smoke, spermatozoa Abstrak: Rokok mengandung bahan kimia yang berbahaya, yaitu nikotin, tar dan gas karbon monoksida (CO). Dalam satu kali hisapan rokok terdapat 1014 molekul radikal bebas atau Reactive Oxygen Species (ROS) yang dapat merusak spermatozoa. Berbagai bahan alam asli Indonesia banyak mengandung antioksidan, salah satunya pada madu. Efek madu sebagai antioksidan dapat melindungi sel-sel tubuh termasuk menetralisir radikal bebas yang disebabkan oleh rokok dan mengurangi kerusakan sel spermatozoa yang disebabkan oleh ROS sehingga menghindari menurunnya kualitas spermatozoa. Penelitian ini bertujuan untuk mengetahui pengaruh madu terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus) yang diberi paparan asap rokok. Subjek penelitian ini menggunakan 9 ekor tikus wistar (Rattus norvegicus) jantan yang dibagi menjadi tiga kelompok secara acak dengan berat badan 150-200 gram dan berumur 12-14 minggu. Masing-masing kelompok tikus diberi paparan asap rokok 2 batang / hari dimana kelompok perlakuan (P1) juga diberi madu 0.5 ml / hari, dan kelompok perlakuan (P2) diberi madu 1 ml / hari. Hasil penelitian memperlihatkan bahwa pemberian madu dapat meningkatkan konsentrasi, motilitas, dan morfologi spermatozoa tikus wistar (Rattus norvegicus) yang diberi paparan asap rokok. Terjadi perbedaan yang bermakna dari konsentrasi, motilitas, dan morfologi spermatozoa kelompok yang hanya diberi paparan asap rokok dan kelompok yang diberi paparan asap rokok dan madu. Hasil tersebut menunjukan bahwa madu 1 ml / hari dapat meningkatkan kualitas spermatozoa. Kata kunci: madu, asap rokok, spermatozoa

The antioxidant effect of mulberry and jamun fruit wines by ameliorating oxidative stress in streptozotocin-induced diabetic Wistar rats

2016 ◽  
Vol 7 (10) ◽  
pp. 4422-4431 ◽  
Author(s):  
Akshatha Hosahalli Srikanta ◽  
Anbarasu Kumar ◽  
Shinde Vijay Sukhdeo ◽  
Muthukumar Serva Peddha ◽  
Vijayalakshmi Govindaswamy
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Wistar Rats ◽  
Metabolic Disorders ◽  
Reactive Oxygen ◽  
Antioxidant Effect ◽  
Oxygen Species

Polyphenols act by scavenging reactive oxygen species during oxidative stress and hence are useful in the treatment of metabolic disorders including diabetes.

scholarly journals Control of Insulin Secretion by Production of Reactive Oxygen Species: Study Performed in Pancreatic Islets from Fed and 48-Hour Fasted Wistar Rats

PLoS ONE ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. e0158166 ◽  
Author(s):  
Ana Cláudia Munhoz ◽  
Patrícia Riva ◽  
Daniel Simões ◽  
Rui Curi ◽  
Angelo Rafael Carpinelli
Keyword(s):  
Reactive Oxygen Species ◽  
Insulin Secretion ◽  
Pancreatic Islets ◽  
Wistar Rats ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Species Study

scholarly journals Virus Mimetic Poly (I:C)-Primed Airway Exosome-like Particles Enter Brain and Induce Inflammatory Cytokines and Mitochondrial Reactive Oxygen Species in Microglia

Biology ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1359
Author(s):  
Deimantė Kulakauskienė ◽  
Deimantė Narauskaitė ◽  
Dovydas Gečys ◽  
Otilija Juknaitė ◽  
Lina Jankauskaitė ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wistar Rats ◽  
Reactive Oxygen ◽  
Microglial Cells ◽  
Poly I:C ◽  
Inflammatory Factors ◽  
Oxygen Species ◽  
The Brain ◽  
Airway Cells

Viral infections induce extracellular vesicles (EVs) containing viral material and inflammatory factors. Exosomes can easily cross the blood-brain barrier during respiratory tract infection and transmit the inflammatory signal to the brain; however, such a hypothesis has no experimental evidence. The study investigated whether exosome-like vesicles (ELVs) from virus mimetic poly (I:C)-primed airway cells enter the brain and interact with brain immune cells microglia. Airway cells were isolated from Wistar rats and BALB/c mice; microglial cell cultures—from Wistar rats. ELVs from poly (I:C)-stimulated airway cell culture medium were isolated by precipitation, visualised by transmission electron microscopy, and evaluated by nanoparticle analyser; exosomal markers CD81 and CD9 were determined by ELISA. For in vitro and in vivo tracking, particles were loaded with Alexa Fluor 555-labelled RNA. Intracellular reactive oxygen species (ROS) were evaluated by DCFDA fluorescence and mitochondrial superoxide—by MitoSOX. ELVs from poly (I:C)-primed airway cells entered the brain within an hour after intranasal introduction, were internalised by microglia and induced intracellular and intramitochondrial ROS production. There was no ROS increase in microglial cells was after treatment with ELVs from airway cells untreated with poly (I:C). In addition, poly (I:C)-primed airway cells induced inflammatory cytokine expression in the brain. The data indicate that ELVs secreted by virus-primed airway cells might enter the brain, cause the activation of microglial cells and neuroinflammation.

scholarly journals Virus Mimetic Poly (I:C)-Primed Airway Exosomes Enter Brain and Induce Mitochondrial Reactive Oxygen Species in Microglia

2021 ◽  
Author(s):  
Deimantė Kulakauskienė ◽  
Deimantė Narauskaitė ◽  
Dovydas Gečys ◽  
Otilija Juknaitė ◽  
Lina Jankauskaitė ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Wistar Rats ◽  
Reactive Oxygen ◽  
Microglial Cells ◽  
Poly I:C ◽  
Inflammatory Factors ◽  
Oxygen Species ◽  
The Brain ◽  
Airway Cells

Viral infections induce exosomes containing viral material and inflammatory factors. During respiratory tract infection, exosomes can easily cross the blood-brain barrier and transmit the inflammatory signal to the brain; however, such a hypothesis has no experimental evidence. The study investigated whether exosomes from virus mimetic poly (I:C)-primed airway cells enter the brain and interact with brain immune cells microglia. Airway cells were isolated from Wistar rats and BALB/c mice; microglial cell cultures - from Wistar rats. Exosomes from poly (I:C)-stimulated airway cell culture medium were isolated by precipitation, visualised by transmission electron microscopy, and evaluated by nanoparticle analyser; exosomal markers CD81 and CD9 were determined by ELISA. For in vitro and in vivo tracking, exosomes were loaded with Alexa Fluor 555-labelled RNA. Intracellular reactive oxygen species (ROS) were evaluated by DCFDA fluo-rescence and mitochondrial superoxide - by MitoSOX. The exosomes from poly (I:C)-primed airway cells entered the brain within an hour after intranasal introduction, were internalised by microglia, and induced intracellular and intramitochondrial ROS production. There was no ROS increase in microglial cells was after treatment with exosomes from airway cells untreated with poly (I:C). The data indicate that virus-primed airway cell exosomes might enter the brain and induce the activation of microglial cells.

Ovariectomy and its Antioxidative Effect on Bone

2010 ◽  
Author(s):  
Durg V. Rai ◽  
Harcharan Singh Ranu
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Free Radicals ◽  
Bone Loss ◽  
Wistar Rats ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Female Wistar Rats ◽  
The Impact

Ovarian hormone deficiency increases the generation of reactive oxygen species. Oxidative stress due to reactive oxygen species (ROS) can cause oxidative damage to cells. Cells have a number of defense mechanisms to protect themselves from the toxicity of ROS. There is increasing evidence of the role of free radicals in bone resorption and bone loss. Ovariectomised female wistar rats had been used as the animal model for the study of osteoporosis. Even though, there are studies portraying the role of free radicals in bone loss, the defense mechanism adapted by bone in ovariectomised animals remains obscure. So, the impact of ovariectomy on the bone antioxidant system in rats was investigated. Twenty female wistar rats were taken and divided into two groups: ovariectomised and control. It had been found that a significant (p<0.001) decrease in the activity of various enzymes like CAT (catalase), SOD (superoxide dismutase) (p<0.001), GST (glutathione-s-transferase). However, an increase in the malondialdehyde levels was found to be 30% in the ovariectomised rats as compared to the controls. Thus the study elucidates the oxidative stress in bone under ovariectomy.

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